Free radical scavenging activity of two medicinal plants used by diabetic patients in Botswana

Motlhanka, D.M.T.; Motlhanka, P.; Matlho, K.

doi:10.1055/s-0028-1083964

Cited by 1 publication

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“…In southern Zimbabwe and Botswana, locals boil ground leaves and twigs to prepare tea that is used to treat respiratory infections [ 23 , 24 ]. The aerial parts of the plant have also been used for the treatment of asthma, inflammation, epilepsy, the heart, wounds, backaches, diabetes, kidney ailments, hypertension, haemorrhoids, gingivitis, shingles, stroke, and skin conditions [ 21 , 25 , 26 ]. M. flabellifolius leaves have flavonoids, alkaloids, terpenoids, proanthocyanidines, condensed tannins, flavan-3-ols, saponins, and phytosterols [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Antioxidant, Cytotoxicity, Antibacterial, Anti-Motility, and Anti-Biofilm Effects of Myrothamnus flabellifolius Welw. Leaves and Stem Defatted Subfractions

Matotoka,

Masoko

2024

Plants

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The formation of biofilms underscores the challenge of treating bacterial infections. The study aimed to assess the antioxidant, cytotoxicity, antibacterial, anti-motility, and anti-biofilm effects of defatted fractions from Myrothamnus flabellifolius (resurrection plant). Antioxidant activity was assessed using DPPH radical scavenging and hydrogen peroxide assays. Cytotoxicity was screened using a brine shrimp lethality assay. Antibacterial activity was determined using the micro-dilution and growth curve assays. Antibiofilm potential was screened using the crystal violet and tetrazolium reduction assay. Liquid–liquid extraction of crude extracts concentrated polyphenols in the ethyl acetate and n-butanol fractions. Subsequently, these fractions had notable antioxidant activity and demonstrated broad-spectrum antibacterial activity against selected Gram-negative and Gram-positive bacteria and Mycobacterium smegmatis (MIC values < 630 μg/mL). Growth curves showed that the bacteriostatic inhibition by the ethyl acetate fractions was through the extension of the lag phase and/or suppression of the growth rate. The sub-inhibitory concentrations of the ethyl acetate fractions inhibited the swarming motility of Pseudomonas aeruginosa and Klebsiella pneumoniae by 100% and eradicated more than 50% of P. aeruginosa biofilm biomass. The polyphenolic content of M. flabellifolius plays an important role in its antibacterial, anti-motility, and antibiofilm activity, thus offering an additional strategy to treat biofilm-associated infections.

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