Free Radical Scavenging Capacities and Alleviating Actions of Polysaccharides Extract of Termitomyces le-testui on Methylprednisolone-Induced Immunodepression in Rats

Ngwenah, Foncham Evans; Antoine, Kada Sanda; Martin, Salah; Tume, Christopher; Mahamat, Oumar

doi:10.1155/2021/5893210

Cited by 2 publications

(2 citation statements)

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“…Spleen and thymus were collected and weighed using an electronic balance. The organ index was calculated using the following formula reported by Evans Ngwenah et al [ 13 ].

…”

Section: Methodsmentioning

confidence: 99%

“…After 2 hrs of incubation, 1N HCl (1 ml) was added and the optical density was read at 540 nm. The mean of the optical densities in treated animal was compared to that of control to evaluate the energy metabolism of the cells [ 13 ]. In addition, nitrite oxide production was measured using the Greiss reagent as reported by Mahamatet al [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

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Antimalarial and Immunomodulatory Activities of Tithonia diversifolia (Asteraceae) Leave Flafonoids‐Rich Extract Used in Cameroonian Traditional Medecine

Ngum,

Masakebenagha,

Mahamat

2024

Scientifica

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Background. Phytochemicals are considered the reliable source for the treatment of infection including malaria. Especially, phenols are known as potentially toxic to the growth and development of pathogens, among which flavonoids are the most extensively studied and play more intensive roles in ethnopharmacology. The immunological effect and role of T. diversifolia flavonoids‐rich extract in treatment of malaria have therefore been examined in this study. Methods. In vitro test against Plasmodium falciparum and 4‐day suppressive and Rane’s tests against Plasmodium berghei in mice were used to evaluate the antimalarial activities. TNF‐α and INF‐γ levels, phagocytic tests, and production of oxygen and nitrogen radical were assessed to appreciate the immunomodulatory activity. One‐way analysis of variance followed by post hoc Student′s t tests was used for data analysis. Results. T. diversifolia flavonoids‐rich extract at the concentrations ranging from 0.0004 mg/ml significantly (p < 0.05) inhibited in a concentration‐dependent manner the growth of trophozoite up to 100% inhibition with 0.025 mg/ml at 24 and 48 hrs. Moreover, T. diversifolia flavonoids‐rich extract reduced the level of parasitemia and improved in a dose‐dependent manner the survival time of infected mice significantly (p < 0.05) compared to their control in 4‐day suppressive test as well as in Rane’s test. Additionally, T. diversifolia flavonoids‐rich extract increased the TNF‐α and INF‐γ levels in rats infected by P. berghei. Furthermore, the flavonoid‐rich extract enhanced weight of spleen in the rats, the metabolic and phagocytic activities of the peritoneal cells, and the concentration of nitric oxide and oxygen radicals in methylprednisolone‐immunocompromised rats compared to the control (p < 0.05). Conclusion. The study has revealed that T. diversifolia flavonoids‐rich extract through its antiplasmodial and phagocytic activities is a promising treatment of malaria.

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“…Spleen and thymus were collected and weighed using an electronic balance. The organ index was calculated using the following formula reported by Evans Ngwenah et al [ 13 ].

…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antimalarial and Immunomodulatory Activities of Tithonia diversifolia (Asteraceae) Leave Flafonoids‐Rich Extract Used in Cameroonian Traditional Medecine

Ngum,

Masakebenagha,

Mahamat

2024

Scientifica

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Analysis of oxidative status, inflammatory cytokines, and Ascaris lumbricoides infection in women at a health district in Bamenda, Northwest, Cameroon

Mugob,

Ngum,

Boubga

et al. 2024

Egypt J Intern Med

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Background Clinical outcomes of ascariasis, one of the most common parasitic infections, are remarkably variable ranging from asymptomatic infection to death. Ascariasis can pair absorption of fats, vitamin A, iodine, and lactose digestion and destroys the villi, with significant consequences in pregnancy outcomes, leading to growth retardation, and cognitive impairment, decreased work capacity, and adverse pregnancy outcomes. One of the crucial factors driving the clinical outcomes of ascariasis is the immune response and associated oxidative stress. This study therefore examined the prevalence of ascariasis and associated immune response dysfunction by measuring four inflammatory cytokines alongside with the oxidant and antioxidant biomarkers in women of reproductive age in a health center in Cameroon. Results Three-hundred and fifty-five women (pregnant and non-pregnant) were examined for the parasite. Because some participants did not donate blood and due to low volume of blood available, only 127 individuals (62 non-pregnant women and 65 pregnant women) were included for analysis of oxidative biomarkers, and 90 samples were used for the evaluation of inflammatory biomarkers (40 non-pregnant and 50 pregnant). The prevalence of Ascaris lumbricoides was of 13.23%. Ascariasis was associated with high levels in inflammatory cytokines (IL-1β, IL-12, IL-10, and TNF-α) and oxidative markers (TOS, OSI, MDA, and CAT) in both pregnant and non-pregnant women, while high level of NO was only seen in pregnant women. A significant relation was observed between some cytokines and oxidant markers: IL-10 and OSI and IL-12 and NO and between IL-1β and MDA in pregnant women, while in non-pregnant, significant relation was found between Il-10 and NO as well as IL-1β and OSI and NO. Principal component analysis (PCA) underlined a pro-inflammatory cytokine signature (with strongest contributions from IL-1β, IL-10, TNF-α). PCA also highlighted an oxidative stress with strongest contributions from TOS, OSI, SOD, NO, and CAT in pregnant and from OSI, TOS, NO, CAT, and TAC in non-pregnant. Conclusion These findings demonstrate elevated cytokines (IL-1β, IL-12, IL-10, and TNF-α) and high oxidative stress imbalance, adding further evidence for the role of a pro-inflammatory cytokine signature of oxidative stress in women with A. lumbricoides.

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Free Radical Scavenging Capacities and Alleviating Actions of Polysaccharides Extract of Termitomyces le-testui on Methylprednisolone-Induced Immunodepression in Rats

Cited by 2 publications

References 33 publications

Antimalarial and Immunomodulatory Activities of Tithonia diversifolia (Asteraceae) Leave Flafonoids‐Rich Extract Used in Cameroonian Traditional Medecine

Antimalarial and Immunomodulatory Activities of Tithonia diversifolia (Asteraceae) Leave Flafonoids‐Rich Extract Used in Cameroonian Traditional Medecine

Analysis of oxidative status, inflammatory cytokines, and Ascaris lumbricoides infection in women at a health district in Bamenda, Northwest, Cameroon

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