Free radical scavenging of grape pomace extracts from Cabernet sauvingnon (Vitis vinifera)

Campos, Luanda Maria Abreu Silva de; Leimann, Fernanda Vitória; Pedrosa, Rozangela Curi; Ferreira, Sandra R.S.

doi:10.1016/j.biortech.2008.02.058

Cited by 165 publications

(104 citation statements)

References 25 publications

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“…They protect plants against harmful environmental conditions and are divided in two main categories, namely, flavonoids and nonflavonoids [31]. The significant correlation between the total polyphenol content and flavonoids content, as observed in this study, was also reported in other studies, in grape pomace extracts [32] and buckwheat extracts [33]. The phytochemical composition of grapes, however, varied not only among the different cultivars, but also on skin or pulp of the same cultivar.…”

Section: Discussionsupporting

confidence: 87%

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Singha

Das

2014

Ind J Clin Biochem

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Grapes are the richest source of antioxidants due to the presence of potent bioactive phytochemicals. In this study, the phytochemical contents, scavenging activities and protective role against H 2 O 2 -induced oxidative stress in liver tissue ex vivo of four grape (Vitis vinifera) cultivars extracts, namely Flame seedless (black), Kishmish chorni (black with reddish brown), Red globe (red) and Thompson seedless mutant (green), were evaluated. The total phenolics and flavonoids content in pulp or skin fractions of different grape cultivars were in the range of 47.6-310 mg gallic acid equivalent/g fresh weight (fw), and 46.6-733.3 lg catechin equivalent/g fw respectively. The scavenging activities in skin of different grape varieties against 2,2-diphenyl-1-picrylhydrazyl (44-58 %), hydrogen peroxide (15.3-18.6 %), and hydroxyl radicals (50-85 %), were higher than pulp of the corresponding cultivars. These scavenging activities of grape extracts were found to be significantly (p \ 0.01) correlated with the levels of total phenols, flavonoids and ascorbic acid. Liver tissues from goat treated with H 2 O 2 (500 lM) showed significantly decreased GSH content by 42.9 % and activities of catalase by 50 % and glutathione reductase by 66.6 %; while increased thiobarbituric acid reactive substances and nitric oxide level by 2.53-and 0.86-fold, respectively, and activity of glutathione S-transferase by 0.96-fold. Grape skin extracts showed the stronger protective activity against H 2 O 2 -induced oxidative stress in liver tissue ex vivo, than its pulp of any cultivar; and the Flame seedless (black) cultivar showed the highest potential. In conclusion, our study suggested that the higher antioxidant potential, phytochemical contents and significant scavenging capacities in pulp and skin of grape extracts showed the protective action of grape extracts against H 2 O 2 -induced oxidative stress in liver tissue ex vivo.

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Section: Discussionsupporting

confidence: 87%

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Singha

Das

2014

Ind J Clin Biochem

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“…The method used for the DPPH radical scavenging was adapted from that of de Campos et al 24 The reaction occurred between 50 lL of sample and 950 lL of 100 lM DPPH. The sample concentrations were in the range of 5-500 lg/mL.…”

Section: Content Of Total Anthocyaninsmentioning

confidence: 99%

Purple Corn (Zea maysL.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

Ramos-Escudero

Muñoz

Alvarado-Ortíz

et al. 2012

Journal of Medicinal Food

138

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This study was designed to determine the contents of total polyphenols, flavonoids, flavonols, flavanols, and anthocyanins of purple corn (Zea mays L.) extracts obtained with different methanol:water concentrations, acidified with 1% HCl (1 N). Another objective was to determine the antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2 0 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and deoxyribose assay, individual phenolic compounds by high-performance liquid chromatography (HPLC), and endogenous antioxidant enzyme (superoxide dismutase [SOD], catalase [CAT], and total peroxidase [TPX]) activity and lipid peroxidation activity (thiobarbituric acidreactive substances [TBARS] assay) in isolated mouse organs. Overall, the highest total content of polyphenols, anthocyanins, flavonoids, flavonols, and flavanols was obtained with the 80:20 methanol:water extract, acidified with 1% HCl (1 N). The 50% inhibitory concentration values obtained by the DPPH and ABTS assays with this extract were 66.3 lg/mL and 250 lg/mL, respectively. The antioxidant activity by the FRAP assay was 26.1 lM Trolox equivalents/g, whereas the deoxyribose assay presented 93.6% inhibition. Because of these results, the 80:20 methanol:water extract, acidified with 1% HCl (1 N), was used for the remaining tests. Eight phenolic compounds were identified by HPLC: chlorogenic acid, caffeic acid, rutin, ferulic acid, morin, quercetin, naringenin, and kaempferol. Furthermore, it was observed that the purple corn extract was capable of significantly reducing lipid peroxidation (lower malondialdehyde [MDA] concentrations by the TBARS assay) and at the same time increasing endogenous antioxidant enzyme (CAT, TPX, and SOD) activities in isolated mouse kidney, liver, and brain. On the basis of the results, it was concluded that the purple corn extract contained various bioactive phenolic compounds that exhibited considerable in vitro antioxidant activity, which correlated well with the decreased MDA formation and increase in activity of endogenous antioxidant enzymes observed in the isolated mouse organs. This warrants further in vivo studies with purple corn extracts to assess its antioxidant activity and other bioactivities.KEY WORDS: antioxidant activity endogenous antioxidant enzymes isolated mouse organs oxidative stress phenolic compounds purple corn Zea mays L.

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“…These solvents may be particularly effective at extracting phenolic and polyphenolic compounds, but are not particularly selective as is the case with ethyl acetate (de Campos et al. 2008) or require long extraction times (Casazza et al. 2012).…”

Section: Introductionmentioning

confidence: 99%

Superheated liquid and supercritical denatured ethanol extraction of antioxidants from Crimson red grape stems

Wenzel

Samaniego

Wang

et al. 2015

Food Science & Nutrition

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Grapes are widely known for health benefits due to their antioxidant content. In wine production, grape stems are often discarded, though they has a higher content of antioxidants than the juice. The effectiveness of using an environmentally friendly solvent, ethanol, as a superheated liquid and supercritical fluid to extract antioxidant compounds from grape stems of organically grown Crimson Seedless grapes was evaluated. The Ferric Reducing Ability of Plasma (FRAP) assay and the Total Phenolic Content (TPC), or Folin‐Ciocalteu assay, were used to quantify the antioxidant power of grape stem extracts. The extractions were performed at temperatures between 160°C and 300°C at constant density. It was found that the optimal extraction temperature was 204°C, at superheated liquid conditions, with a FRAP value of 0.670 mmol Trolox Equivalent/g of dry grape stem. The FRAP values were higher than other studies that extracted antioxidants from grape stems using single‐pass batch extraction.

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Free radical scavenging of grape pomace extracts from Cabernet sauvingnon (Vitis vinifera)

Cited by 165 publications

References 25 publications

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Purple Corn (Zea maysL.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

Superheated liquid and supercritical denatured ethanol extraction of antioxidants from Crimson red grape stems

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