Free Radical Scavenging, Reducing Power and Lipid Peroxidation Inhibition Activities of M. communis Berries Methanol Extract and its Fractions

Abstract

Free radicals or highly reactive oxygen species are capable of inducing oxidative damage to the human body Plants containing phenolic compounds have potent antioxidant capacity. The present study was undertaken to investigate the in vitro antioxidant activities of Myrtus communis L. (Myrtaceae), a plant widely used as natural remedy for digestive disorders in folk medicine. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 Method. EAE extract showed the highest polyphenolic and flavonoids contents (358.37±2.28 GAE/g of dry extract and 105.44±3.48 QE/g of dry extract) respectively. The EAE had the highest antioxidant activity as measured by DPPH radical and hydroxyl radical scavenging activity. EAE and ME extracts exhibited the highest reducing power. EAE possess an IC50 close to BHT (0.074 mg/ml) as reference drug All extracts exhibited antioxidant activity in the linoleic acid emulsion system (76.81% -86.93 %). EAE showed an inhibition ratio of (86.93 %) close to that of BHT (94.9±1.52 %). These findings provide evidence that Myrtus communis L. berries are a potential source of antioxidant which have many benefits towards human health.

Chemical Composition and Antibacterial and Antioxidant Activities of Stem Bark Essential Oil and Extracts of Solanecio gigas

“…Furthermore, the DPPH radical is a free radical that has been widely used as a tool to estimate the free radical scavenging activity of antioxidants. When antioxidants interact with DPPH, they either transfer electrons or hydrogen atoms to it, neutralizing its free-radical nature [ 19 , 46 ]. In the present study, EO and methanolic extract showed higher or lower antioxidant activities as compared to the standard ascorbic acid (Figures 3 and 4 ).…”

“…Using the method described earlier, the antioxidant activity of the EO and methanol extract was determined by their ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) stable radicals [ 19 ]. 3 mL of methanolic extract and 1 mL of freshly prepared 0.1 mM DPPH methanolic solution were combined thoroughly and kept in the dark for 60 minutes.…”

Fabrication of a Biocompatible Nanoantimicrobial Suture for Rapid Wound Healing after Surgery

“…In the β-carotene-linoleic acid assay system, the method is based on the ability of the test solutions to decrease the oxidative losses of β-carotene in a β-carotene/linoleic acid emulsion . Similar to the DPPH test, the extract had the highest inhibition value for the co-oxidation reactions of linoleic acid and β-carotene at 10 mg/mL concentration, with 97.94 ± 3.00%.…”

Free Radical Scavenging and Antinociceptive Activities of the Aqueous Extract from Matricaria chamomilla L. Flowers.

“…Many extraction factors play important roles in the extraction efficiency, such as type and concentration of extraction solvent, extraction temperature, extraction time, and extraction pH. Among them, the solvent is one of the most influential factors (Xu et al, 2017;Benchikh et al, 2022). It has also been proven that the quantities and nature of phenolic compounds in plants are related to climatic conditions, altitude and the characteristics of soil (Benabdallah et al, 2020).…”