Free radicals in antigen formation: reduction of contact allergic response to hydroperoxides by epidermal treatment with antioxidants

Gäfvert, Elisabeth; Nilsson, Jonas; Hagelthorn, Gunnel; Karlberg, Ann‐Therése

doi:10.1046/j.1365-2133.2002.04664.x

Cited by 22 publications

(9 citation statements)

References 14 publications

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“…Irritants have been shown to induce the release of ROS event at non-cytotoxic concentrations [6]. It has also been mentioned that generation of ROS may be relevant in case allergens bind protein through a radical-mediated pathway like using limonene hydroperoxide [27]. Contact dermatitis also causes a potent increase of iron level in acute and chronic lesional area, as well in healthy skin [28].…”

Section: Resultsmentioning

confidence: 99%

“…The rational basis for the application of antioxidants and metal chelators in dermatology is well documented [6,23,27]. The inflammatory response following acute UV irradiation and the degenerative processes related to chronic UV radiation skin exposure are largely mediated by the overproduction of ROS, such as superoxide anion and hydrogen peroxide, and by impairment of the antioxidant endogenous system.…”

Section: Resultsmentioning

confidence: 99%

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In vivo Skin Irritation Potential of a Castanea sativa (Chestnut) Leaf Extract, a Putative Natural Antioxidant for Topical Application

Almeida¹,

Valentão

Andrade

et al. 2008

Basic Clin Pharma Tox

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Topical application of natural antioxidants has proven to be effective in protecting the skin against ultravioletmediated oxidative damage and provides a straightforward way to strengthen the endogenous protection system. However, natural products can provoke skin adverse effects, such as allergic and irritant contact dermatitis. Skin irritation potential of Castanea sativa leaf ethanol:water (7:3) extract was investigated by performing an in vivo patch test in 20 volunteers. Before performing the irritation test, the selection of the solvent and extraction method was guided by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and polyphenols extraction (measured by the Folin Ciocalteu assay). Iron-chelating activity and the phenolic composition (high performance liquid chromatography/diode array detection) were evaluated for the extract obtained under optimized conditions. The extraction method adopted consisted in 5 short extractions (10 min.) with ethanol:water (7:3), performed at 40 ° . The IC 50 found for the iron chelation and DPPH scavenging assays were 132.94 ± 9.72 and 12.58 ± 0.54 μ g/ml (mean ± S.E.M.), respectively. The total phenolic content was found to be 283.8 ± 8.74 mg GAE/g extract (mean ± S.E.M.). Five phenolic compounds were identified in the extract, namely, chlorogenic acid, ellagic acid, rutin, isoquercitrin and hyperoside. The patch test carried out showed that, with respect to irritant effects, this extract can be regarded as safe for topical application.Many plant extracts are used in cosmetic formulations as additives (fragrances, colorants, etc.) or are used due to their functional properties (emollient, anti-irritant, moisturizing, slimming, anti-ageing, etc.). Botanical products are also used in topical pharmaceutical preparations in a wide variety of skin disorders. However, allergic and irritant contact dermatitis and phytophotodermatitis are included among the topical adverse effects of natural products [1]. Members of the Ranunculaceae, Euphorbiaceae and Asteraceae (Compositae) plant families are specially involved in irritant contact dermatitis. Phytophotodermatitis is caused by plants containing furocoumarins, which notably include the members of the Umbelifereae, Rutaceae and Moraceae plant families. Allergic contact dermatitis is induced primarily by plants from the Asteraceae family, which contain sesquiterpene lactones, but other families containing this class of compounds, specially the Lauraceae, Magnoliaceae and Jubulaceae families, can also produce allergic reactions. Besides studying the activity of plant extracts, suitable tolerance studies are therefore required before any possible practical application.The skin is exposed to a broad variety of biological, chemical and physical attacks. Among them is solar ultraviolet (UV) radiation that is responsible for skin damage, which includes erythema, photoageing and cancer. Following UV exposure, reactive oxygen species (ROS) are produced and are believed to be largely responsible for some o...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

In vivo Skin Irritation Potential of a Castanea sativa (Chestnut) Leaf Extract, a Putative Natural Antioxidant for Topical Application

Almeida¹,

Valentão

Andrade

et al. 2008

Basic Clin Pharma Tox

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“…This protective effect could be of interest in humans, but only as a possible treatment for patients exposed to haptens that yield antigens via a radical mechanism. For haptens that form antigens by means of nucleophilic-electrophilic reactions, this effect has no benefit [191].…”

Section: Other Compoundsmentioning

confidence: 99%

Effects of Natural Products on Contact Dermatitis

Rı́os¹,

Bas

Recio

2005

CMCAIAA

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Some medicinal plants, which are known to produce allergic reactions, are also specifically used as antiinflammatory agents. Among the more relevant plants, we report species with cinnamaldehyde, cinnamic alcohol, geraniol, hydroxycitronellal, eugenol and isoeugenol are all potential allergens. In addition, fragrances, which are mixtures of small-molecular-weight compounds, may induce allergic contact dermatitis due to fragrance-specific CD4 + and CD8 + T lymphocytes. Plants from the Asteraceae family used in folk medicine as anti-inflammatories can cause allergic contact dermatitis because of its content in sesquiterpene lactones, which have been reported as the antiinflammatory principles in this species. Species with flavonoids, iridoids, terpenoids and alkaloids have been described as inhibitors of contact dermatitis. Scrophularia auriculata, Poria cocos, Santolina chamaecyparissus, Ranunculus sceleratus and Helichrysum italicum all showed activity in different experimental protocols of contact dermatitis, thus justifying the potential use of these medicinal plants as anti-allergens and inhibitors of contact dermatitis reactions produced by allergens and chemicals. Hydroquinone derivatives such as 1-O-β-glucopyranosyl-2-(3'-hydroxymethyl-3'-methylallyl) hydroquinone and arbutin, flavonoids such as kaempferol, apigenin and genistein, sesquiterpene lactones such as helenalin, diterpenes such as triptonide, triterpenes such as tripterine and bryonolic acid, iridoids such as scrovalentinoside, alkaloids such as indirubin, dehydrocorydaline, magnoflorine hydroxide and phellodendrine acetate, and polysaccharides such as fucoidin have been reported as inhibitors of contact dermatitis reactions.

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“…Neutrophil derived reactive oxygen intermediates, such as hydrogen peroxide and superoxide radicals, are responsible for the pathogenesis of various inflammatory conditions (Halliwell, 1994;Gafvert et al, 2002;Mazzetti et al, 2001;Hanson and Clegg, 2002;Loukides et al, 2002). The antiinflammatory properties of various medicinal plant extracts have been explained, at least in part, by their antioxidant activities (Gerritsen et al, 1995;Gali-Muhtasib et al, 1999;Schinella et al, 2002;Lindsey et al, 2002).…”

Section: Introductionmentioning

confidence: 97%

The antioxidant potential of Sutherlandia frutescens

Fernandes¹,

Cromarty²,

Albrecht³

et al. 2004

Journal of Ethnopharmacology

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Free radicals in antigen formation: reduction of contact allergic response to hydroperoxides by epidermal treatment with antioxidants

Abstract: The protective effect of antioxidants in elicitation could be of practical therapeutic value, as it indicates a possibility for the treatment of patients who have become sensitized to haptens that form full antigens via a radical mechanism.

Cited by 22 publications

References 14 publications

In vivo Skin Irritation Potential of a Castanea sativa (Chestnut) Leaf Extract, a Putative Natural Antioxidant for Topical Application

In vivo Skin Irritation Potential of a Castanea sativa (Chestnut) Leaf Extract, a Putative Natural Antioxidant for Topical Application

Effects of Natural Products on Contact Dermatitis

The antioxidant potential of Sutherlandia frutescens

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