The inheritance of antioxidant activity (AOA) and its association with seedcoat color was investigated in cowpea [Vigna unguiculata (L.) Walp.]. Four advanced cowpea lines, ARK95-356 (black seedcoat) and ARK98-348 (red seedcoat), which were high (H) in AOA, and ARK96-918 (cream seedcoat) and LA92-180 (cream seedcoat), which were low (L) in AOA, were selected from the 2002 Regional Southernpea Cooperative Trials. They were crossed in a complete diallel mating design, generating F 1 , F 1´ (1st generation and 1st generation reciprocal cross, respectively), F 2 , F 2´ (2nd generations from F 1 , F 1´) , BC 1 , and BC 2 (backcrosses to parents 1 and 2, respectively) populations. Individual seeds were ground and samples were extracted in methanol and analyzed for AOA using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Combining ability tests using Griffi ngʼs Method I Model I indicated presence of highly signifi cant general combining ability (GCA), specifi c combining ability (SCA), and reciprocal (RE) and maternal (MAT) effects, with pigmented lines exhibiting positive GCA and MAT, while nonpigmented lines exhibited negative GCA and MAT. AOA in the F 1 was not signifi cantly different from the maternal parent, with seedcoat color also resembling the maternal parent. Segregation for seedcoat color was observed in the F 2 and F 2´. Additive, dominance, and epistatic effects were signifi cant. The broad sense heritability estimate was 0.87. Minimum number of genes responsible for AOA was estimated at fi ve. Factors governing high AOA appeared to be the same as those responsible for seedcoat color, with apparent pleiotropic effects. In conclusion, breeding for high AOA in cowpea is possible using highly pigmented parental lines.