Free-range chickens from Santa Catarina state, southern Brazil, as asymptomatic intermediate hosts for Toxoplasma gondii clonal type I and typical Brazilian genotypes

Pena, Hilda Fátima Jesus; Soares, Herbert Sousa; Oliveira, Solange; Ferreira, Marina Neves; Bricarello, Patrízia Ana; Machado, Thiago Mombach Pinheiro; Castro, Bruno Bello Pede; Gennari, Solange Maria

doi:10.1016/j.vprsr.2018.04.001

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…In addition, the remaining 44 B1positive samples were amplified at only 3-5 loci, so have limited significance to reveal the level of genetic variation of T. gondii. To date, although different genotypes of T. gondii have been reported in domestic avian species worldwide (e.g., ToxoDB#2, 9, 10, 26,53,114,225,227,278,281,282) (19,(48)(49)(50)(51)(52), ToxoDB#9 is the prominent genotype in domestic poultry in China, and has been also frequently reported in other animals in China (53). A previous study (19) indicated that only one genotype (ToxoDB#9) was identified from domestic ducks, suggesting that the genetic variation of T. gondii may be relatively low in domestic ducks in China.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Toxoplasma gondii and Neospora caninum in Domestic Ducks in Hunan Province, China

Zheng

Yang

et al. 2021

Front. Vet. Sci.

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Toxoplasma gondii and Neospora caninum are protozoan parasites that infect warm-blooded animals, and cause major economic losses in livestock industries worldwide. However, little is known about the genotypes of T. gondii and N. caninum in domestic ducks in China. Herein, brain samples from 588 domestic ducks from Hunan province in China were examined for the presence of T. gondii and N. caninum. Polymerase chain reaction (PCR) was used to detect T. gondii B1 gene and N. caninum NC-5 gene. Forty-five DNA samples (7.7%; 95% CI: 5.5–9.9) were positive for B1 gene, and two (0.3%; 95% CI: 0–0.7) were positive for NC-5 gene. The risk factors significantly associated with T. gondii infection were age and sex. The 45 samples positive for T. gondii were genotyped using multi-locus PCR-RFLP analysis and only one sample was fully genotyped as ToxoDB#9 (Chinese I). These results provide new information about the epidemiology of T. gondii and N. caninum in ducks in Hunan province in China. The data also highlight the importance of a “One Health” approach to dealing with toxoplasmosis.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Toxoplasma gondii and Neospora caninum in Domestic Ducks in Hunan Province, China

Zheng

Yang

et al. 2021

Front. Vet. Sci.

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“…Large genetic variability of T. gondii in Brazil has been shown both in animal and human reservoirs, which could be implicated in the clinical disease outcome. Recent studies in Brazil predominantly reported diverse new non-archetypal genotypes; the most common single genotype found was BrIII and it was identified in chickens, horses and wild mammals (Fournier et al ., 2014; Silva et al ., 2018; Pena et al ., 2018 a ) in addition a type I genotype, although rare, was also described (Pena et al ., 2018 b ). Finding such diverse strains supports the need for further studies comparing specific and cross protection immune responses raised against them by the hosts.…”

Section: Discussionmentioning

confidence: 99%

Early immune responses and parasite tissue distribution in mice experimentally infected with oocysts of either archetypal or non-archetypal genotypes of Toxoplasma gondii

et al. 2020

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“…In our study, we had an isolation rate of 14.3%, with two isolates obtained. Previous studies with free-range chickens form Brazil have described an isolation ranging from 15.12 to 87.87% [20][21][22]. In the present study, isolates was obtained from seropositive animals; however, previous studies have described isolation of the parasite from seronegative animals, including chickens and doves [23,24].…”

Section: Resultsmentioning

confidence: 60%

Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil

Silva

Barros

et al. 2020

Acta Parasit.

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The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. Methods Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR-RFLP. Results Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database. Conclusions Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.

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Free-range chickens from Santa Catarina state, southern Brazil, as asymptomatic intermediate hosts for Toxoplasma gondii clonal type I and typical Brazilian genotypes

Cited by 12 publications

References 25 publications

Molecular Detection of Toxoplasma gondii and Neospora caninum in Domestic Ducks in Hunan Province, China

Molecular Detection of Toxoplasma gondii and Neospora caninum in Domestic Ducks in Hunan Province, China

Early immune responses and parasite tissue distribution in mice experimentally infected with oocysts of either archetypal or non-archetypal genotypes of Toxoplasma gondii

Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil

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