Free zone electrophoresis

Hjertén, Stellan

doi:10.1016/0009-5907(67)80003-6

Cited by 676 publications

(233 citation statements)

References 53 publications

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“…The wafers were then rinsed with DI water and dried in nitrogen. Prior to membrane fabrication, the glass channels were coated with an acrylate-terminated self-assembling monolayer to enable covalent attachment of the polyacrylamide membrane to the channel walls (Kirby et al 2003;Hjerten 1967Hjerten , 1985. For this, the channels were prepared by exposing to 1 M HCl for 30 min, rinsing in DI water, and then exposing to 1 M NaOH for 30 min.…”

Section: Membrane Fabrication and Surface Treatmentmentioning

confidence: 99%

Integrated microfluidic preconcentrator and immunobiosensor

Kondapalli

Connelly

Baeumner

et al. 2011

Microfluid Nanofluid

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We present a microfluidic biosensor that integrates membrane-based preconcentration with fluorescence detection. The concentration membrane was fabricated in polyacrylamide by an in situ photopolymerization technique at the junction of glass microchannels. Liposomes entrapping sulforhodamine B dye molecules were used for signal amplification. The biotin-streptavidin binding system was a model system for evaluating device performance. Biotinylated liposomes were preconcentrated at the membrane by applying an electric field across the membrane. The electric field causes the liposomes to migrate toward the membrane where they are concentrated by a sieving effect. Two orders of magnitude concentration was achieved after applying the electric field for only 2 min. The concentrated bolus was then eluted toward the detection unit, where the biotinylated liposomes were captured by immobilized streptavidin. The integrated system with the preconcentration module shows a 14-fold improvement in signal as opposed to a system that does not include preconcentration.

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Section: Membrane Fabrication and Surface Treatmentmentioning

confidence: 99%

Integrated microfluidic preconcentrator and immunobiosensor

Kondapalli

Connelly

Baeumner

et al. 2011

Microfluid Nanofluid

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“…In normal free-zone electrophoresis (Hjerten, 1967) electro-endosmosis can be reduced or even suppressed by coating the inner walls of the electrophoretic chamber. The best results and practical elimination of the electro-endosmosis effect have been obtained by a double-coating procedure described by Smith & Ware (1978).…”

Section: Electro-endosmosismentioning

confidence: 99%

Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry

Hoffmann¹,

Kaufmann²,

Steiner³

et al. 1981

Br J Cancer

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Determination of the electrophoretic mobility of test cells has been widely used in an attempt to detect so-called lymphokines in a laboratory test for cancer, but operational difficulties are inherent in conventional cytopherometers. This study therefore investigates the technical and operational aspects of cell electrophoresis, using the Zeiss cytopherometer; e.g. influence of electro-osmosis, focus uncertainty, movement due to convection and other sources of error. Implications and possible improvements in the test are discussed.

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“…The advantages of ion analysis using CE have included resolution, speed, simplicity, and reduced sample preparation, compared to ion chromatography. In cation separations by CE, Hjertén first reported for the separation of Bi 3+ and Cu 2+ with lactic acid as complexing agent [3]. From this work, many papers have been published on the analysis of alkali and alkaline earth metals, and ammonium ion [4][5][6] in the early 1990s.…”

Section: Introductionmentioning

confidence: 99%

Effect of perfluorinated surfactant as additive of background electrolyte for capillary electrophoresis of inorganic cations

Mori¹,

Ikeda²,

Itabashi³

2012

Acta Chromatographica

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Summary. This study reports the effect of a nonionic perfluorinated surfactant, N-polyoxyethylene-N-propyl perfluorooctane sulfonamide (PFOSA), as additive of background electrolyte on capillary electrophoresis (CE) of common inorganic cations. The association constants (K ass ) for PFOSA estimated from the electrophoretic mobility of analyte cations were the order of Mg 2+ > Ca 2+ > Sr 2+ >> K + ≈ NH 4 + > Na + ≈ Li + . The K ass values were larger than those for zwitterionic and nonionic surfactants with hydrocarbon moiety. Use of PFOSA made another essential contribution to the determination of inorganic cations in a protein-containing sample. This was considered because high solubility of PFOSA for proteins functioned as suppressor for protein adsorption to the capillary wall. Four inorganic cations, Na + , K + , Mg 2+ , and Ca 2+ , in human saliva sample were successfully determined by sample injection without any pretreatments except for filtration and dilution.

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Free zone electrophoresis

Cited by 676 publications

References 53 publications

Integrated microfluidic preconcentrator and immunobiosensor

Integrated microfluidic preconcentrator and immunobiosensor

Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry

Effect of perfluorinated surfactant as additive of background electrolyte for capillary electrophoresis of inorganic cations

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