Freeform Vertical and Horizontal Fabrication of Alginate-Based Vascular-Like Tubular Constructs Using Inkjetting

Xu, Changxue; Zhang, Zhengyi; Christensen, Kyle; Huang, Yong; Fu, Jianzhong; Markwald, Roger R.

doi:10.1115/1.4028578

Cited by 50 publications

(37 citation statements)

References 22 publications

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“…If the nozzle moving direction is parallel to the longitudinal axis of tubular structures, the fabrication process is called horizontal printing; if the nozzle moves along the circumferential direction of tubular structures, the fabrication process is called vertical printing (Xu et al, 2014b). In light of previous inkjet bioprinting successes (Boland et al, ; Nishiyama et al, ; Xu et al, ; Xu et al, ; Xu et al, ), the inkjetting technology has been further explored to fabricate 3D vascular‐like structures with both horizontal and vertical bifurcations in this study.…”

Section: Introductionmentioning

confidence: 99%

Freeform inkjet printing of cellular structures with bifurcations

Christensen

Chai

et al. 2015

Biotech & Bioengineering

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309

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Organ printing offers a great potential for the freeform layer-by-layer fabrication of three-dimensional (3D) living organs using cellular spheroids or bioinks as building blocks. Vascularization is often identified as a main technological barrier for building 3D organs. As such, the fabrication of 3D biological vascular trees is of great importance for the overall feasibility of the envisioned organ printing approach. In this study, vascular-like cellular structures are fabricated using a liquid support-based inkjet printing approach, which utilizes a calcium chloride solution as both a cross-linking agent and support material. This solution enables the freeform printing of spanning and overhang features by providing a buoyant force. A heuristic approach is implemented to compensate for the axially-varying deformation of horizontal tubular structures to achieve a uniform diameter along their axial directions. Vascular-like structures with both horizontal and vertical bifurcations have been successfully printed from sodium alginate only as well as mouse fibroblast-based alginate bioinks. The post-printing fibroblast cell viability of printed cellular tubes was found to be above 90% even after a 24 h incubation, considering the control effect.

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Section: Introductionmentioning

confidence: 99%

Freeform inkjet printing of cellular structures with bifurcations

Christensen

Chai

et al. 2015

Biotech & Bioengineering

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309

228

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“…Drop-on-demand (DOD) technologies for micro-droplet deposition have been widely used in various fields owing to high precision and automation [1][2][3][4][5]; applications range from additive manufacturing [6][7][8][9][10] and electronics applications [11,12] to emerging fields such as pharmacology [13], pathology [14], tissue engineering [15][16][17][18], and biosensor manufacturing [19]. For instance, DOD mode micro-droplet deposition has been employed for high-throughput screening (HTS) applications to increase the efficiency of drug screening and delivery [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Zhao

Yan

Yao

et al. 2015

Journal of Manufacturing Science and Engineering

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AbstractsDrop-on-demand (DOD) micro-droplet formation and deposition play an important role in additive manufacturing, particularly in printing of 3D in vitro biological models for pharmacological and pathological studies, for tissue engineering and regenerative medicine applications, and for building of cell-integrated micro-fluidic devices. In development of a DOD based micro-droplet deposition process for 3D cell printing, the droplet formation, controlled on-demand deposition and at the single cell level, and most importantly, maintaining the viability and functionality of the cells during and after the printing, are all remaining to be challenged. This report presents our recent study on developing a novel DOD based micro-droplet deposition process for 3D Printing by utilization of an alternating viscous & inertial force jetting mechanism. The results include an analysis of droplet formation mechanism, the system configuration, and experimental study of the effects of process parameters on micro-droplet formation. Sodium Alginate solutions are used for micro-droplet formation and deposition. Key process parameters include actuation signal waveforms, nozzle dimensional features, and solution SUN A c c e p t e d M a n u s c r i p t N o t C o p y e d i t e dviscosity. Sizes of formed micro-droplets are examined by measuring the droplet diameter and velocity. Resultsshow that by utilizing a nozzle at a 45μm diameter, the size of the formed micro-droplets are in the range of 52~72μm in diameter and 0.4~2.0m/s in jetting speed, respectively. Reproducibility of the system is also examined and the results show that the deviation of the formed micro-droplet diameter and the droplet deposition accuracy are within 6% and 6.2μm range, respectively. Experimental results demonstrate a high controllability and precision for the developed DOD micro-droplet deposition system with a potential for precise cell printing.

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“…The ECM provides structural and biochemical support to the living cells. In this study, the sodium alginate hydrogel was selected as the ECM environment due to their inherent hydrophilic properties [3,4,20,[31][32][33][34]. The NIH/3T3 mouse fibroblast was selected as the living cell [19,[35][36][37].…”

Section: Methodsmentioning

confidence: 99%

“…Zhang et al [20] classified two types of ligament flow during inkjet-based printing of cell-laden bioink and further studied their effects on cell distribution inside the microspheres. Xu et al [3,4] and Christensen et al [21] investigated the manufacturing challenges during inkjet-based printing of 3D vascular constructs including falling down due to moment imbalance, and axially varying and cross-sectional deformations due to droplet impaction. Xu et al [22] investigated the effects of voltage on droplet velocity and volume for the electric field-assisted droplet formation process during inkjet-based 3D printing.…”

Section: Droplet Formation Inmentioning

confidence: 99%

“…According to a report by Grand View Research, the global 3D bioprinting market is expected to grow 26.5% annually by 2021 to reach $1.82 billion by 2022. Recent advances in bioprinting have enabled tissue engineers to print complex functional living tissues and organs using bioink materials consisting of extracellular matrix (ECM) and living cells [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

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Predictive Modeling of Droplet Formation Processes in Inkjet-Based Bioprinting

2018

Journal of Manufacturing Science and Engineering

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Additive manufacturing is driving major innovations in many areas such as biomedical engineering. Recent advances have enabled three-dimensional (3D) printing of biocompatible materials and cells into complex 3D functional living tissues and organs using bio-printable materials (i.e., bioink). Inkjet-based bioprinting fabricates the tissue and organ constructs by ejecting droplets onto a substrate. Compared with microextrusion-based and laser-assisted bioprinting, it is very difficult to predict and control the droplet formation process (e.g., droplet velocity and volume). To address this issue, this paper presents a new data-driven approach to predicting droplet velocity and volume in the inkjet-based bioprinting process. An imaging system was used to monitor the droplet formation process. To investigate the effects of polymer concentration, excitation voltage, dwell time, and rise time on droplet velocity and volume, a full factorial design of experiments (DOE) was conducted. Two predictive models were developed to predict droplet velocity and volume using ensemble learning. The accuracy of the two predictive models was measured using the root-mean-square error (RMSE), relative error (RE), and coefficient of determination (R2). Experimental results have shown that the predictive models are capable of predicting droplet velocity and volume with sufficient accuracy.

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Freeform Vertical and Horizontal Fabrication of Alginate-Based Vascular-Like Tubular Constructs Using Inkjetting

Abstract: F re e fo rm V e rtic a l and H o rizo n ta l F a b ric a tio n of A lg in a te -B a s e d V a s c u la r-L ik e T u b u la r C onstructs U sing In k je ttin g

Cited by 50 publications

References 22 publications

Freeform inkjet printing of cellular structures with bifurcations

Freeform inkjet printing of cellular structures with bifurcations

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Predictive Modeling of Droplet Formation Processes in Inkjet-Based Bioprinting

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