Freestanding biopellet electrodes based on carbon nanotubes and protein compression for direct and mediated bioelectrocatalysis

Stretchable Photonic Crystal-Assisted Glycoprotein Identification for Ovarian Cancer Diagnosis

“…EY and glycoprotein ion association by electrostatic attraction formed between objects showed the strongest fluorescence quenching ability. On the contrary, glycoproteins GOD (pI 4.2), OVA (pI 4.6–4.9), TRF (pI 5.2–5.9), , HAase (pI 5.0–5.9), and AchE (pI 7.5) bind to the dye mainly through hydrophobic interaction because there are not enough cations to bind to EY anion. Ion associations are formed under physiological conditions, resulting in fluorescence quenching of the system.…”

“…Different glycoproteins form complexes with EY dyes with varying degrees of reduced fluorescence intensity, indicating that EY has good selectivity for glycoproteins. In the pH 7.4 PBS buffer solution and biological matrix, EY has two negative charges, and glycoproteins such as IgG (pI 9.95) 20 and HRP (pI 9.1) 21 had "strong" positive charges before their isoelectric points. EY and glycoprotein ion association by electrostatic attraction formed between objects showed the strongest fluorescence quenching ability.…”

Encapsulation of multiple enzymes within a microgel via water-in-water emulsions for enzymatic cascade reactions

“…The molecular weights of these enzymes were 40, 150, and 540 kDa, and their respective IEP values were 7.2, 4.2, and 4.6. 38,39 To study their encapsulation, each enzyme was labelled with the fluorescent dyes FITC-HRP, RhoB-GOD, and DNS-b-Gal, and the completion of purification was confirmed by size-exclusion chromatography with a fluorescence detector (Fig. S7, ESI †).…”

Chlorhexidine digluconate exerts bactericidal activity vs Gram positive Staphylococci with bioelectrocatalytic compatibility: High level disinfection for implantable biofuel cells

“…Following our previously reported procedure, MWCNT redox biopellet electrodes (⌀ = 6 mm, ca. 1.8 ± 0.2 mm thick) were obtained by soft grinding of the following mixtures [43] : (i) GOx-1,4-NQ-cat bioanode: 50 µL distilled H2O, 7.5 mg of GOx; 5 mg of cat, 2.5 mg of 1,4-NQ, and 17.5 mg of CNTs; (ii) BOx-FePP biocathode: 50 µL distilled H2O, 7.5 mg of BOx; 2.5 mg of FePP, and 17.5 mg of CNTs. The resulting homogeneous pastes were then compressed into disks using a manual hydraulic press (Specac, Eurolabo) with a pressure of ca.…”