Freezability biomarkers in the epididymal spermatozoa of swamp buffalo

Salinas, Marvin Bryan Segundo; Lertwichaikul, Teepakorn; Khunkaew, Chakorn; Boonyayatra, Sukolrat; Sringarm, Korawan; Chuammitri, Phongsakorn; Sathanawongs, Anucha

doi:10.1016/j.cryobiol.2022.04.005

Cited by 7 publications

(8 citation statements)

References 45 publications

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“…As this and other previous studies indicate, LPO compromises the integrity of the plasma membrane, affects sperm function and may also lead to motility impairment and the induction of apoptotic-like changes [ 9 , 81 ]. Whereas the greater LPO observed in the sperm membrane of the PFEs compared to the GFEs observed in the present work was similar to that found in frozen–thawed bovine sperm [ 82 ] and frozen–thawed epididymal sperm from buffaloes [ 83 ], Gómez-Fernández et al [ 84 ] detected similar LPO and ROS levels in pig sperm when PFEs and GFEs were compared. Such disparities could be attributed to the post-thawing time of the samples used for clustering the GFEs and PFEs (immediately after thawing in horses, cattle and buffaloes; 150 min after thawing in pigs) or to interspecies differences.…”

Section: Discussionsupporting

confidence: 89%

“…While cryopreservation induces OS in the sperm of horses [ 86 , 87 , 88 , 89 ], cattle [ 90 , 91 , 92 , 93 ] and cats [ 94 ], there is still controversy as to whether it induces OS in the sperm of pigs [ 67 , 94 , 95 , 96 , 97 , 98 , 99 ] and dogs [ 100 , 101 , 102 ]. Interestingly, the association observed between increased membrane LPO and decreased sperm quality after thawing was also found in other studies on horses [ 62 , 75 , 88 , 103 ], buffaloes [ 83 , 104 , 105 , 106 ], cattle [ 82 ], humans [ 107 , 108 ], sheep [ 109 , 110 ] and even birds [ 111 ] and fish [ 112 ]. In some of them, it was also noted that the increase in LPO was concomitant with an increase in ROS [ 75 , 82 , 83 , 88 , 104 , 105 , 107 , 108 ].…”

Section: Discussionsupporting

confidence: 76%

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Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Catalán,

Yánez-Ortiz,

Torres-Garrido

et al. 2024

Antioxidants

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Cryopreservation is a stressful process for sperm, as it is associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create an imbalance with antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether the membrane LPO and DNA fragmentation of frozen–thawed horse sperm relies upon antioxidant activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and paraoxonase type 1 (PON1)); non-enzymatic antioxidant capacity (Trolox-equivalent antioxidant capacity (TEAC), plasma ferric reducing antioxidant capacity (FRAP) and cupric reducing antioxidant capacity (CUPRAC)); and the oxidative stress index (OSI) of their seminal plasma (SP). Based on total motility and plasma membrane integrity (SYBR14+/PI−) after thawing, ejaculates were hierarchically (p < 0.001) clustered into two groups of good- (GFEs) and poor-(PFEs) freezability ejaculates. LPO and DNA fragmentation (global DNA breaks) were higher (p < 0.05) in the PFE group than in the GFE group, with LPO and DNA fragmentation (global DNA breaks) after thawing showing a positive relationship (p < 0.05) with SP OSI levels and ROS production. In addition, sperm motility and membrane integrity after thawing were negatively (p < 0.05) correlated with the activity levels of SP antioxidants (PON1 and TEAC). The present results indicate that LPO and DNA fragmentation in frozen–thawed horse sperm vary between ejaculates. These differences could result from variations in the activity of antioxidants (PON1 and TEAC) and the balance between the oxidant and antioxidant components present in the SP.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 76%

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Catalán,

Yánez-Ortiz,

Torres-Garrido

et al. 2024

Antioxidants

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“…To understand whether the ejaculates within each bull are significantly different, samples at other time points must be taken from the same bulls. Salinas et al [ 22 ] found that the response of swamp buffalo sperm to freeze-thaw procedures was not always the same among bulls. In contrast, however, Cardoso et al [ 23 ] found that, in dogs, there was no significant within-male or among-male difference in post-thaw semen motility.…”

Section: Resultsmentioning

confidence: 99%

Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches

Mokhtassi‐Bidgoli

Sharafi²,

Benson

2023

Animals

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Cryo-injury reduces post-thaw semen quality. Extender components play a protective role, but existing experimental approaches do not elucidate interactions among extender components, semen samples, and post-thaw quality. To identify optimal concentrations for 12 extender ingredients, we ran 122 experiments with an I-optimal completely random design using a large dataset from our previous study. We obtained a maximum predicted total motility of 70.56% from an I-optimal design and 73.75% from a Monte Carlo simulation. Individual bull variations were significant and interacted with extenders independently. 67% of bulls reliably preferred extender formulations to reach maximum motility. Multifactor analysis suggests that some antioxidants may offer superior protection over others. Partial least squares path modeling (PLS-PM) found the highest positive loadings for glutathione in the antioxidant class, glycerol in the CPA class, and fructose in the basic compounds class. The optimal ranges for milk, water, and ethylene glycol were extremely narrow. Egg yolk, cholesterol-loaded cyclodextrin, and nerve growth factor had medium-loading impacts. PLS-PM showed that CPA, osmoregulators, and basic components were the most efficient contributors to motility, while the antioxidant and extracellular protectant classes had less efficiency. Thus, ingredients, concentrations, and interactions of extender compounds are critical to extender formulation, especially when using multiple compounds with the same function.

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“…A study published by Jakop et al [ 43 ] showed that detached acrosomes characteristic of spermatozoa from “poor freezer” bulls are a consequence of changes in the lipid composition of the sperm membranes and altered antioxidant capacity of the seminal fluid. Malondialdehyde (MDA) levels, as a consequence of lipid peroxidation (LPO), were shown to be significantly lower in good freezers when compared to poor freezers [ 44 ]. The abundance of polyunsaturated fatty acids (PUFAs) in sperm membranes as well as seminal fluid [ 44 , 45 ] were also significantly correlated with cryoresistance.…”

Section: Oxidative Markersmentioning

confidence: 99%

“…Malondialdehyde (MDA) levels, as a consequence of lipid peroxidation (LPO), were shown to be significantly lower in good freezers when compared to poor freezers [ 44 ]. The abundance of polyunsaturated fatty acids (PUFAs) in sperm membranes as well as seminal fluid [ 44 , 45 ] were also significantly correlated with cryoresistance. Likewise, an inverse association was found for monounsaturated fatty acid (MUFA) concentration.…”

Section: Oxidative Markersmentioning

confidence: 99%

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

Ďuračka

Benko

Tvrdá

2023

IJMS

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For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well as humans. Nevertheless, the success of cryopreservation varies across species, seasons, and latitudes and even within the same individual. With the dawn of progressive analytical techniques in the field of genomics, proteomics, and metabolomics, new options for a more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics of spermatozoa that could predict their cryotolerance before the freezing process. Understanding the changes in sperm biology as a result of their exposure to low temperatures may contribute to the development and implementation of appropriate measures to assure high post-thaw sperm quality. Furthermore, an early prediction of cryotolerance or cryosensitivity may lead to the establishment of customized protocols interconnecting adequate sperm processing procedures, freezing techniques, and cryosupplements that are most feasible for the individual needs of the ejaculate.

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Freezability biomarkers in the epididymal spermatozoa of swamp buffalo

Cited by 7 publications

References 45 publications

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

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