Freezability, enzyme leakage and fertility of buffalo spermatozoa in relation to the quality of semen ejaculates and extenders

Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa

“…Its extracellular leakage has negatively been correlated with fertilizing ability of sperm and could be used as a marker of membrane damage during freezing. Similarly, the release of glutamic oxalacetic transaminase (GOT) is associated with cryodamages in buffalo sperm [45]. In the present study, extracellular leakages of LDH and GOT were recorded lowest in buffalo sperm cryopreserved in the extender with 10% and 15 % pigeon egg yolk compared to 20 % chicken egg yolk (controls).…”

Seminal Plasma: An Essential Attribute to Spermatozoa

“…Seminal ALP activity is observed on sperm head, midpiece, and tail fragments, and it is known to regulate phosphorylation of proteins by the cAMP‐dependent protein kinase necessary for spermatozoal motility (Tang and Hoskins, 1975). Dhami and Kodagali (1990) stated that ALP and GOT could be used as markers for freezability and fertility in buffalo because they observed a negative correlation between ALP leakage and motility of cryopreserved buffalo spermatozoa. Upreti et al (1996) proposed ALP leakage in SP as a marker for optimization of cooling and freezethaw steps of cryopreservation for ram semen.…”

Factors Affecting the Quality of Cryopreserved Buffalo (Bubalus bubalis) Bull Spermatozoa

“…(1986) found that Tris–citric acid based extender is suitable for the cryopreservation of buffalo spermatozoa in terms of post‐thaw motility and survivability. Later on, Dhami and Kodagali (1990) studied the effects of semen extenders based on Tris or citrate buffer. It was reported that Tris‐based extender improved the freezability of buffalo spermatozoa as judged by the extracellular release of spermatozoal enzymes and in vivo fertility.…”