Freeze‐dried platelet‐rich plasma shows beneficial healing properties in chronic wounds

Abstract

Fresh platelet concentrates are used in many centers to treat recalcitrant wounds. To extend the therapeutic shelf-life of platelets, we analyzed the wound-healing effects of fresh-frozen and freeze-dried (FD) platelet-rich plasma (PRP) using a diabetic mouse model. Db/db mice with 1.0 cm2 dorsal excisional wounds (n = 15/group) were treated with a single application of FD PRP (1.2 x 10(6) platelets/microL) with or without a stabilization solution, and compared with wounds treated with fresh-frozen, sonicated PRP, and untreated wounds. Granulation tissue area, thickness, and wound size were analyzed 9 days posttreatment. Immunostained sections were quantified for vascularity and proliferation using antiplatelet endothelial cell adhesion molecule I and antiproliferating cell nuclear antigen antibodies. The results showed that all PRP preparations increased granulation tissue formation as assessed by surface coverage, thickness, and angiogenic response, when compared with untreated wounds. In addition, wounds treated with FD PRP, and biochemically stabilized FD PRP, exhibited higher proliferative levels. The possibility to deliver growth factors using platelets, and the potential to extend the shelf-life of platelet concentrates makes freeze-drying methods particularly suitable for enhanced wound care.

Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

“…PRP has been demonstrated to be effective in bone regeneration, wound healing, and improvement of musculoskeletal sports injuries. [9][10][11][12][13][14][15] Recently, clinicians extended the scope of PRP therapy to soft tissue augmentation by combining PRP with fat grafting. Although some successful clinical results were reported, 16,17 evidence supporting the application of PRP combined with fat grafts in soft tissue augmentation remains limited, as only a few basic research and preclinical studies in small animals have been conducted.…”

Platelet-Rich Plasma Application for Acute Tympanic Membrane Perforations

“…There have been human studies to report that PRP has a positive effect on wound healing [12][13][14][15] . In animal studies, histological examinations revealed enhanced fibroblastic and endothelial cell formation during wound healing, increased neovascularization [12,[19][20][21][22] , and enhanced granulation tissue formation [12,19,21,22] .…”

Bio Technological Intervention with Platelet Rich Plasma for Assisted Regeneration of Sole

“…There are many cases reported in different studies showing encouraging trends for PRP to be used as local application in the form of gel for promoting the healing of chronic wounds. But all these studies have used PRP as local application, adjunct to standard ulcer management [29,30]. However, these gels are expensive.…”

“…These growth factors aid healing by attracting un-differentiated cells in the newly formed matrix and triggering cell division. PRP may suppress cytokine release and limit inflammation, interacting with macrophages to improve tissue healing and regeneration, promote new capillary growth, and accelerate epithelialization in chronic wounds [29,30].…”