1987
DOI: 10.1177/35.12.3680932
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Freeze-fracture cytochemistry of rat glomerular capillary tuft. Determination of wheat germ agglutinin binding sites and localization of anionic charges.

Abstract: We propose here the use of freeze-fracture to gain access and to label in vitro glomerular components and locate WGA receptors and anionic sites. Tissues are frozen, fractured under liquid nitrogen, and thawed. Freeze-fracture rendered all glomerular structures directly accessible to the reagents. This made possible study of the nature and topology of cationized ferritin and WGA binding sites. WGA-gold complexes were observed over plasma membranes of podocytes and of endothelial and mesangial cells. Labeling o… Show more

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Cited by 4 publications
(1 citation statement)
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“…However, this lectin labels both Nacetylglucosamine and N-acetylneuraminic acid (Chevalier et al 1987). Limax flavus agglutinin (LFA), which is used in our present study to determine specific sugar elements on the mesothelium, has higher specificity for sialic acid (Miller et al 1982;Roth et al 1984).…”
Section: Introductionmentioning
confidence: 99%
“…However, this lectin labels both Nacetylglucosamine and N-acetylneuraminic acid (Chevalier et al 1987). Limax flavus agglutinin (LFA), which is used in our present study to determine specific sugar elements on the mesothelium, has higher specificity for sialic acid (Miller et al 1982;Roth et al 1984).…”
Section: Introductionmentioning
confidence: 99%