2017
DOI: 10.1111/nyas.13460
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Freezing does not alter multiscale tendon mechanics and damage mechanisms in tension

Abstract: It is common in biomechanics to use previously frozen tissues, where it is assumed that the freeze-thaw process does not cause consequential mechanical or structural changes. We have recently quantified multiscale tendon mechanics and damage mechanisms using previously frozen tissue, where damage was defined as an irreversible change in the microstructure that alters the macroscopic mechanical parameters. Because freezing has been shown to alter tendon microstructures, the objective of this study was to determ… Show more

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Cited by 39 publications
(37 citation statements)
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“…Rat tail tendon fascicle was dissected following the same protocol from published studies (Lee & Elliott, ; Lee et al. ).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Rat tail tendon fascicle was dissected following the same protocol from published studies (Lee & Elliott, ; Lee et al. ).…”
Section: Methodsmentioning
confidence: 99%
“…Rat tail tendon fascicle was dissected following the same protocol from published studies (Lee & Elliott, 2017;. Plantaris and Achilles tendons were dissected using bone-cutting shears, scalpels, and forceps to dissect the Achilles and plantaris complex, about 4 cm in length with the calcaneus attached ( Fig.…”
Section: Gross Morphologymentioning
confidence: 99%
“…Rat tail tendon was dissected following the same protocol from a published study. 32,33 Plantaris and Achilles tendons were dissected using bone cutting shears, scalpels, and forceps to dissect the Achilles and plantaris complex about 4 cm in length with the calcaneus attached. We separated out both plantaris and Achilles from each other by removing excess surrounding soft tissue.…”
Section: Methodsmentioning
confidence: 99%
“…4 All tendons had been frozen at −20C, and the maximum number of the freeze-thaw cycle was limited to three to minimize the effect of freezing. 32 A total summary of sample number and dimensions, including cross-sectional-area and gauge length, are included as a supplemental material (Fig S-1).…”
Section: Methodsmentioning
confidence: 99%
“…10 The rat tail tendon was harvested as previously described. 32 All tendons were placed in PBS at room temperature to keep hydrated. We stained rat plantaris and tail tendons with 10 μ g/ml 5-DTAF (5-(4,6-Dichlorotriazinyl) aminofluorescein, Life Technologies) and Achilles tendon with 20 μ g/ml 5-DTAF for 20 minutes.…”
Section: Methodsmentioning
confidence: 99%