Freezing of Nonwoody Plant Tissues

Finkle, Bernard J.; Pereira, E. Sa B.; Brown, Milford S.

doi:10.1104/pp.53.5.705

Cited by 13 publications

(4 citation statements)

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“…Rottenburg Interpretation of FR-1 and FR-2 as representing the freezing of extracellular and cellular liquids, respectively, may provide an explanation of the cell-protective effect of ultraslow cooling, as reported in the preceding paper (4). The gross transfer of water out of the cell shown by Figure 3 of that paper (4) presumably concentrated the cellular contents and lowered its freezing temperature (FR-2) so that the cells no longer froze at the -4 C terminal temperature of those experiments. It would also follow from this interpretation that at just a few degrees below -4 C, a damaging degree of freezing would be expected, even under conditions of ultraslow cooling.…”

Section: Discussionmentioning

confidence: 99%

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Freezing of Nonwoody Plant Tissue

Brown

Pereira²,

Finkle³

1974

Plant Physiol.

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MATERIALS AND METHODSCylinders measuring 5 mm in diameter were punched from fresh fruits or vegetables with a cork borer, trimmed to a length of 20 mm, and used without washing. Temperature at the center was measured with a thermistor mounted in a 24-gauge hypodermic needle and recorded as described previously (4). Tissues studied included primarily the parenchyma of cucumber (Cucumis sativus), peach (Prunus persica) and cantaloupe (Cucumis melo cantalupensis). Other tissues studied are listed in Table I. Specimens were frozen by immersion in octafluorocyclobutane (Freon C-318,' b.p. -5.8 C).In order to observe the effect of degree of sample hydration upon the shape of the freezing curve, weighed cylinders cut longitudinally from cucumber fruits were held for 3 hr at constant relative humidity over saturated salt solutions (NaCl, 76% RH5; Mg(NO4),, 54% RH; KCO,CH,, 25% RH) or over distilled water at room temperature. In some experiments, this treatment was followed by rehydration in water for 2 hr prior to freezing.Tests for determining degree of cell rupture by loss of pigments or electrolytes, and degrees of softening by increased compressibility were described previously (3). RESULTSTypical freezing curves for cylindrical sections of cucumber are shown in Figure 1. Initiation of freezing was followed by the first freezing region (FR-1) or plateau. After a temperature drop, there was usually a second freezing region (FR-2) of reduced slope, although not necessarily a plateau, with a great number of temperature spikes and a shape characteristic of the tissue. The variability in intensity of these spikes in freezing curves of a number of other edible tissues is presented in Table I. Figure 2 shows some of the variations possible in these freezing curves (Fig. 2A)

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Section: Discussionmentioning

confidence: 99%

“…Temperature at the center was measured with a thermistor mounted in a 24-gauge hypodermic needle and recorded as described previously (4). Tissues studied included primarily the parenchyma of cucumber (Cucumis sativus), peach (Prunus persica) and cantaloupe (Cucumis melo cantalupensis).…”

Section: Methodsmentioning

confidence: 99%

Freezing of Nonwoody Plant Tissue

Brown

Pereira²,

Finkle³

1974

Plant Physiol.

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“…There are two classes of cryoprotectants viz. penetrating and non-penetrating (Finkle et al, 1985;Benson, 2008a). Penetrating cryoprotectants can move across cell membranes and in so doing, prevent ice crystal formation and membrane rupture during freezing (Wolfe et al, 2002;de Lara Janz et al, 2012).…”

Section: Cryoprotectionmentioning

confidence: 99%

“…Despite their ability to enhance vitrification, scavenge ROS and enhance tissue survival after cooling, cryoprotectants can also be cytotoxic to plant tissues (Kistnasamy et al, 2011;Best, 2015). Cell death may occur as a result of intracellular solute accumulation and/or plasma membrane collapse due to changes in osmotic pressure associated with the dehydrating properties of cryoprotectants (Finkle et al, 1985). In an attempt to cryopreserve explants from E. capensis, Hajari et al (2011) reported improved survival after the explants were cryoprotected with a combination of penetrating cryoprotectants (DMSO and glycerol).…”

Section: Cryoprotectionmentioning

confidence: 99%

Responses to chilling and cryopreservation of recalcitrant seeds of Ekebergia capensis from different provenances

Bharuth

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I, Vishal Bharuth declare that 1. The research reported in this thesis, except where otherwise indicated, is my original research.2. This thesis has not been submitted for any degree or examination at any other university.3. This thesis does not contain other persons' data, pictures, graphs or other information, unless specifically acknowledged as being sourced from other persons.4. This thesis does not contain other persons' writing, unless specifically acknowledged as being sourced from other researchers. Where other written sources have been quoted, then: a. Their words have been re-written but the general information attributed to them has been referenced b. Where their exact words have been used, then their writing has been placed in italics and inside quotation marks, and referenced. 5. This thesis does not contain text, graphics or tables copied and pasted from the Internet, unless specifically acknowledged, and the source being detailed in the thesis and the References sections.

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Low temperature storage of plant tissue cultures

Withers

1980

Advances in Biomedical Engineering, Volume 18

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Freezing of Nonwoody Plant Tissues

Cited by 13 publications

References 4 publications

Freezing of Nonwoody Plant Tissue

Freezing of Nonwoody Plant Tissue

Responses to chilling and cryopreservation of recalcitrant seeds of Ekebergia capensis from different provenances

Low temperature storage of plant tissue cultures

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