Freezing of stored, chilled dog spermatozoa

Hermansson, Ulrika; Forsberg, Catharina Linde

doi:10.1016/j.theriogenology.2005.06.004

Cited by 39 publications

(44 citation statements)

References 25 publications

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“…These results are different to those previously obtained by Hermansson and Linde Forsberg (2006) who found no differences in the membrane status of spermatozoa immediately frozen after collection or after 24 and 48 hours of chill-storage. It has been shown that sperm cryopreservation is accompanied by an increase in the amount of reactive oxygen species, probably due to modifications in the antioxidant balance of the spermatozoa (Strzezek and others 2012).…”

Section: Discussioncontrasting

confidence: 99%

“…Unfortunately, few studies have been performed to evaluate the sperm viability of frozen-thawed canine sperm chilled for several days before freezing. The first experience was performed by Hermansson and Linde Forsberg (2006) who demonstrated that semen samples could be frozen within 48 hours of cold storage in a refrigerator at 4°C. This study was replicated by Ponglowhapan and others (2006) using epididymal sperm and by Santana and others (2013) comparing several times of cold storage in a refrigerator prior to freezing.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of canine semen after cold storage in a Neopor box: effect of extender, centrifugation and storage time

et al. 2014

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The aim of this work was to assess the combined effect of sperm centrifugation, semen extender and storage time before freezing on post-thaw sperm quality and freezability on chilled stored canine semen in a Neopor box. Sperm parameters evaluated were total and progressive sperm motility by Computer-Assisted Sperm Analysis (CASA) and sperm viability and acrosome integrity using a triple fluorescent stain. Sperm quality and freezability indexes were also studied. First, the effect of centrifugation and two commercial extenders from Minitübe (Biladyl A and CaniPRO Freeze A) was evaluated in chilled semen after 24 and 45 hours of cold storage. No significant differences were observed between treatments in almost all the sperm parameters assessed. Secondly, chilled semen was frozen after 24 and 45 hours of cold storage in a Neopor box. The best results were obtained when semen was centrifuged, chilled with CaniPRO Freeze A and then frozen after 24 hours of cold storage, showing no differences in both post-thaw sperm quality and freezability in comparison with semen immediately frozen after collection. In conclusion, dog semen centrifuged after collection and extended with CaniPRO Freeze can be frozen after 24 hours of cold storage in a Neopor box, obtaining similar results to semen immediately frozen after collection.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryopreservation of canine semen after cold storage in a Neopor box: effect of extender, centrifugation and storage time

et al. 2014

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“…According to our results, no differences were found in total sperm motility during chill storage; only progressive sperm motility was lower after 96 hours of chill storage. This is in agreement with previous studies where long-term chill storage of dog semen did not change the motility of chilled dog semen up to 96 hours [29,38]. However, in the present study, we observed an increment of sperm DNA fragmentation just after 48 hours of chill storage.…”

Section: Discussionsupporting

confidence: 94%

DNA integrity of canine spermatozoa during chill storage assessed by the sperm chromatin dispersion test using bright-field or fluorescence microscopy

et al. 2015

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“…The four ejaculates were each time pooled, in order to increase semen volume and eliminate variability between the evaluated samples (Verstegen et al, 2005;Hermansson & Linde Forsberg, 2006), the pool was then analysed.…”

Section: Collection and Evaluation Of Ejaculatesmentioning

confidence: 99%

Canine sperm vitrification with sucrose: effect on sperm function

et al. 2011

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The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra-rapid cryopreservation in canine sperm was investigated. Swim-up selected spermatozoa of second-fraction semen were vitrified with different concentrations of sucrose (0.1, 0.25 and 0.4 m) in proportion 1 : 1 v/v with HTF-BSA 1%. From each group, 30-μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by submerging the spheres in HTF with 1% BSA at 37 °C. The number of progressively motile spermatozoa was significantly higher in the sucrose 0.25 m + HTF-BSA 1% (42.5 ± 2.3%, P < 0.01) than in HTF only (1.66 ± 0.3%). The same combination of sucrose 0.25 m + HTF-BSA 1% (42.7 ± 1.5%) had a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P < 0.05) and decreased the DNA fragmentation (2.8 ± 0.5%) as compared with HTF only (1.93 ± 0.6% and 5.6 ± 0.6% respectively). With respect to acrosome-reacted spermatozoa, no significant difference was found between the groups investigated (P > 0.05). It is concluded that sucrose, a nonpermeable cryoprotectant, can effectively preserve important physiological parameters such as mitochondrial membrane potential and DNA integrity during ultra-rapid cryopreservation.

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Freezing of stored, chilled dog spermatozoa

Cited by 39 publications

References 25 publications

Cryopreservation of canine semen after cold storage in a Neopor box: effect of extender, centrifugation and storage time

Cryopreservation of canine semen after cold storage in a Neopor box: effect of extender, centrifugation and storage time

DNA integrity of canine spermatozoa during chill storage assessed by the sperm chromatin dispersion test using bright-field or fluorescence microscopy

Canine sperm vitrification with sucrose: effect on sperm function

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