Freezing tolerant tobacco, transformed to accumulate osmoprotectants

Konstantinova, T.; Parvanova, D.; Atanassov, A.; Djilianov, Dimitar

doi:10.1016/s0168-9452(02)00090-0

Cited by 136 publications

(85 citation statements)

References 37 publications

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“…Transgenic tobacco (Nicotiana tabacum) overexpressing the p5cs gene that encodes P5CS produced 10-to 18-fold more proline and exhibited better performance under salt stress (Kishor et al 1995). Freezing tolerance was achieved by transforming tobacco with the same gene (p5cs; Konstantinova et al 2002). Because P5CS is the rate-limiting enzyme in proline biosynthesis in plants, and is subject to feedback inhibition by proline, removal of the feedback inhibition can result in high level of proline accumulation .…”

Section: Applications Of Compatible-solute Engineeringmentioning

confidence: 99%

“…Therefore, up-regulation of the de novo synthesis of choline to increase glycine betaine synthesis is also imported in non-accumulators, which express foreign choline-oxidizing enzymes (Nuccio et al 1998;Huang et al 2000). In addition, correct subcellular targeting of the inserted gene also has a significant impact on its expression and expected functions (Sakamoto et al 1998;Konstantinova et al 2002).…”

Section: Applications Of Compatible-solute Engineeringmentioning

confidence: 99%

See 1 more Smart Citation

Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance

Wang

Vinocur

Altman

2003

Planta

3,080

1,709

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Abiotic stresses, such as drought, salinity, extreme temperatures, chemical toxicity and oxidative stress are serious threats to agriculture and the natural status of the environment. Increased salinization of arable land is expected to have devastating global effects, resulting in 30% land loss within the next 25 years, and up to 50% by the year 2050. Therefore, breeding for drought and salinity stress tolerance in crop plants (for food supply) and in forest trees (a central component of the global ecosystem) should be given high research priority in plant biotechnology programs. Molecular control mechanisms for abiotic stress tolerance are based on the activation and regulation of specific stress-related genes. These genes are involved in the whole sequence of stress responses, such as signaling, transcriptional control, protection of membranes and proteins, and free-radical and toxic-compound scavenging. Recently, research into the molecular mechanisms of stress responses has started to bear fruit and, in parallel, genetic modification of stress tolerance has also shown promising results that may ultimately apply to agriculturally and ecologically important plants. The present review summarizes the recent advances in elucidating stress-response mechanisms and their biotechnological applications. Emphasis is placed on transgenic plants that have been engineered based on different stress-response mechanisms. The review examines the following aspects: regulatory controls, metabolite engineering, ion transport, antioxidants and detoxification, late embryogenesis abundant (LEA) and heat-shock proteins.

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Section: Applications Of Compatible-solute Engineeringmentioning

confidence: 99%

Section: Applications Of Compatible-solute Engineeringmentioning

confidence: 99%

Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance

Wang

Vinocur

Altman

2003

Planta

3,080

1,709

View full text Add to dashboard Cite

show abstract

“…In overwintering plants, the accumulated fructans are the main carbon source for survival under persistent snow cover and for spring growth until sufficient photosynthetic activities are resumed (Yukawa and Watanabe 1991). In addition to being a carbohydrate reserve, fructans are thought to play roles in protection against environmental stresses such as low temperature and drought (Hendry 1993;Pilon-Smits et al 1995;Konstantinova et al 2002). Accumulation of water-soluble carbohydrates including fructans in winter cereals during cold hardening has been shown to be associated with winter survival (Olien 1984;Pontis 1989;Tognetti et al 1990; Mohammad et al 1997;Yoshida et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat

Kawakami

Yoshida

2005

Planta

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Fructans play important roles not only as a carbon source for survival under persistent snow cover but also as agents that protect against various stresses in overwintering plants. Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L.) during cold hardening. We detected fructan: fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100) activity for catalyzing the formation and extension of beta-(2,1)-linked fructans in hardened wheat tissues, cloned cDNAs (wft3 and wft4) of 1-FFT, and analyzed the enzymatic properties of a wft3 recombinant protein (Wft3m) produced by yeast. Wft3m transferred beta-(2,1)-linked fructosyl units to phlein, an extension of sucrose through beta-(2,6)-linked fructosyl units, as well as to inulin, an extension of sucrose through beta-(2,1)-linked fructosyl units, but could not efficiently synthesize long inulin oligomers. Incubation of a mixture of Wft3m and another recombinant protein of wheat, sucrose:fructan 6-fructosyltransferase (6-SFT), with sucrose and 1-kestotriose produced fructans similar to those that accumulated in hardened wheat tissues. The results demonstrate that 1-FFT produces branches of beta-(2,1)-linked fructosyl units to phlein and graminan oligomers synthesized by 6-SFT and contributes to accumulation of fructans containing beta-(2,1)- and beta-(2,6)-linked fructosyl units. In combination with sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and 6-SFT, 1-FFT is necessary for fructan synthesis in hardened wheat.

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“…19 a modification of a single enzyme in a biochemical pathway is usually contrasted by a tendency of plant cells to restore homeostasis. 13 Targeting multiple steps in a pathway through the manipulation of a transcription factor has the potential of modifing metabolite fluxes in a more predictable manner. In particular, the manipulation of the activity of transcription factors that control guard cell movements is expected to be useful to develop plants able to consume less water and to maintain high yield in conditions of water scarcity.…”

mentioning

confidence: 99%