Freezing under pressure: A new method for cryopreservation

Greer, Nickolas

doi:10.1016/j.cryobiol.2014.12.005

Cited by 24 publications

(8 citation statements)

References 17 publications

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“…[ 29 ] The elevated solute concentration due to ice formation also results in irreversible injury to cells after undergoing freeze–thaw cycles. [ 30,31 ] To date, cryopreservation strategies are mainly divided into the slow freezing and vitrification methods. [ 5 ] For the most common and traditional slow‐freezing techniques, despite allowing low‐CPA solution, ice injury both in intracellular and extracellular solutions is a crucial factor for cell survival.…”

Section: Introductionmentioning

confidence: 99%

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Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

2021

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Cryopreservation technology has developed into a fundamental and important supporting method for biomedical applications such as cell‐based therapeutics, tissue engineering, assisted reproduction, and vaccine storage. The formation, growth, and recrystallization of ice crystals are the major limitations in cell/tissue/organ cryopreservation, and cause fatal cryoinjury to cryopreserved biological samples. Flourishing anti‐icing materials and strategies can effectively regulate and suppress ice crystals, thus reducing ice damage and promoting cryopreservation efficiency. This review first describes the basic ice cryodamage mechanisms in the cryopreservation process. The recent development of chemical ice‐inhibition molecules, including cryoprotectant, antifreeze protein, synthetic polymer, nanomaterial, and hydrogel, and their applications in cryopreservation are summarized. The advanced engineering strategies, including trehalose delivery, cell encapsulation, and bioinspired structure design for ice inhibition, are further discussed. Furthermore, external physical field technologies used for inhibiting ice crystals in both the cooling and thawing processes are systematically reviewed. Finally, the current challenges and future perspectives in the field of ice inhibition for high‐efficiency cryopreservation are proposed.

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Section: Introductionmentioning

confidence: 99%

“…[29] The elevated solute concentration due to ice formation also results in irreversible injury to cells after undergoing freeze-thaw cycles. [30,31] To date, cryopreservation strategies are mainly divided into the slow freezing and vitrification methods. [5] For the Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

2021

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“…Consequently, the growth rate of ice crystals is reduced by high pressure. At atmospheric pressure, freezing causes ice crystals with rough surfaces, resulting in mechanical injury to the membrane of cells and tissues [58]. On the other hand, the surface of ice crystals at high pressure is much smoother and could possibly cause less mechanical damage to the cell membrane [59].…”

Section: High Hydrostatic Pressurementioning

confidence: 99%

Technologies for Vitrification Based Cryopreservation

Amini

Benson

2023

Bioengineering

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Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy, tissue engineering, transplantation, reproductive technologies, and bio-banking. Among diverse cryopreservation methods, significant focus has been placed on vitrification due to low cost and reduced protocol time. However, several factors, including the intracellular ice formation that is suppressed in the conventional cryopreservation method, restrict the achievement of this method. To enhance the viability and functionality of biological samples after storage, a large number of cryoprotocols and cryodevices have been developed and studied. Recently, new technologies have been investigated by considering the physical and thermodynamic aspects of cryopreservation in heat and mass transfer. In this review, we first present an overview of the physiochemical aspects of freezing in cryopreservation. Secondly, we present and catalog classical and novel approaches that seek to capitalize on these physicochemical effects. We conclude with the perspective that interdisciplinary studies provide pieces of the cryopreservation puzzle to achieve sustainability in the biospecimen supply chain.

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“…The isochoric cryopreservation is a two-phase equilibrium process, in which ice and liquid exist simultaneously at equilibrium under constant temperature and volume, while hyperbaric cryopreservation the solution is maintained in a single phase as liquid, the survival rate is however low in this method [34]. RBCs were successfully cryopreserved by this method [35]. Despite multiple attempts, scientists have not been able to cryopreserve and restore normal functions of complex bio-samples, such as mammalian tissues and organs.…”

Section: Isochoric and Hyperbaric Cryopreservationmentioning

confidence: 99%

Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models

Aljaser¹

2022

Veterinary Medicine and Science

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The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involving animal and human diseases. Cryopreservation has emerged as promising technique for fertility preservation and assisted reproduction techniques (ART) for production of animal breeds and genetically engineered animal species for research. Slow rate freezing and rapid freezing/vitrification are the two main methods of cryopreservation. Slow freezing is characterized by the phase transition (liquid turning into solid) when reducing the temperature below freezing point. Vitrification, on the other hand, is a phenomenon in which liquid solidifies without the formation of ice crystals, thus the process is referred to as a glass transition or ice-free cryopreservation. The vitrification protocol applies high concentrations of cryoprotective agents (CPA) used to avoid cryoinjury. This chapter provides a brief overview of fundamentals of cryopreservation and established methods adopted in cryopreservation. Strategies involved in cryopreserving germ cells (sperm and egg freezing) are included in this chapter. Last section describes the frontiers and advancement of cryopreservation in some of the important animal models like rodents (mouse and rats) and in few large animals (sheep, cow etc).

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Freezing under pressure: A new method for cryopreservation

Cited by 24 publications

References 17 publications

Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

Technologies for Vitrification Based Cryopreservation

Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models

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