Frenemies of the soil: Bacillus and Pseudomonas interspecies interactions

Lyng, Mark; Kovács, Ákos T.

doi:10.1016/j.tim.2023.02.003

Cited by 32 publications

(24 citation statements)

References 99 publications

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“…Competitive interactions on the one hand are vital for the maintenance of ecological diversity and evolution, and on the other hand, they act as an ecological force that play an important role in microbial metabolism and ecological and evolutionary diversity ( Day and Young, 2004 ). Any net negative interaction seen in a laboratory four-species co-cultivation model can possibly be equilibrated into a competitive balance in a natural setting where numerous other species coexist ( Kehe et al, 2021 ; Lyng and Kovács, 2023 ). Nevertheless, natural interactions are often far more complex than cultivations in the laboratory so sometimes it is difficult to predict bacterial behavior in a natural setting from laboratory trials.…”

Section: Discussionmentioning

confidence: 99%

Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Sadiq¹,

Reu²,

Burmølle

et al. 2023

Front. Microbiol.

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Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to the identification of a large number (327) of highly diverse bacterial species on food contact surfaces of the dairy, meat, and egg industries after routine cleaning and disinfection (C&D) regimes. In the current study, biofilm-forming ability of 92 bacterial strains belonging to 26 genera and 42 species was assessed and synergistic interactions in biofilm formation were investigated by coculturing species in all possible four-species combinations. Out of the total 455 four-species biofilm combinations, greater biofilm mass production, compared to the sum of biofilm masses of individual species in monoculture, was observed in 34 combinations. Around half of the combinations showed synergy in biofilm mass > 1.5-fold and most of the combinations belonged to dairy strains. The highest synergy (3.13-fold) was shown by a combination of dairy strains comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, and Calidifontibacter indicus. The observed synergy in mixed biofilms turned out to be strain-specific rather than species-dependent. All biofilm combinations showing remarkable synergy appeared to have certain common species in all combinations which shows there are keystone industry-specific bacterial species which stimulate synergy or antagonism and this may have implication for biofilm control in the concerned food industries.

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Section: Discussionmentioning

confidence: 99%

Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Sadiq¹,

Reu²,

Burmølle

et al. 2023

Front. Microbiol.

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“…In infections, pyoverdine and enterobactin are essential for P. aeruginosa and E. coli, respectively, not only due to iron requirements in a complex environment, but also in polymicrobial interactions as interspecies competitive factors [74]. Siderophores are also thought to play a role in biocontrol of plant pathogens by both Pseudomonas and Bacillus, but a thorough examination of the literature reveals that Bacillus and Pseudomonas siderophores have only rarely been directly implicated in interspecies interactions in planta [13]. Most evidence originated in the previous millennium where Pseudomonas siderophores proved to be determinants of microbial interactions through iron sequestration [75,76].…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus and Pseudomonas are bacterial genera with high environmental impacts. They are environmentally ubiquitous and often co-isolated, especially within the context of crops, where they function as biostimulants and biocontrol agents, but also, in the case of Pseudomonas, as phytopathogens [13].…”

Section: Introductionmentioning

confidence: 99%

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Lyng

Jørgensen

Schostag

et al. 2023

Preprint

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Siderophores have long been implicated in sociomicrobiology as determinants of bacterial interrelations. For plant-associated genera like Bacillus and Pseudomonas, siderophores are well known for their biocontrol functions. Here, we explored the functional role of the Bacillus subtilis siderophore bacillibactin in an antagonistic interaction with Pseudomonas marginalis. The presence of bacillibactin strongly influenced the outcome of the interaction in an iron-dependent manner. The bacillibactin producer B. subtilis restricts colony spreading of P. marginalis by repressing the transcription of histidine kinase-encoding gene gacS, thereby abolishing production of secondary metabolites such as pyoverdine and viscosin. By contrast, lack of bacillibactin restricted B. subtilis colony growth in a mechanism reminiscent of a siderophore tug-of-war for iron. Our analysis revealed that the Bacillus-Pseudomonas interaction is conserved across fluorescent Pseudomonas spp., expanding our understanding of the interplay between two genera of the most well-studied soil microbes.

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“…Bacillus and Pseudomonas are recognized as suitable for a biocontrol method because of their prevalence in diverse ecological, resilience, and persistence capabilities. Furthermore, they also deliver a variety of bioactive substances [21]. The majority of these bioactive substances consist of secondary metabolites that exert detrimental effects on pathogens, directly or indirectly influencing processes such as antibiosis, competition, plant growth stimulation, and defense [22].…”

Section: In Vitro Antagonism Of P Palmivora By Bacillus and Pseudomonasmentioning

confidence: 99%

Exploring the antagonist potential of indigenous Trichoderma spp., Bacillus, and Pseudomonas against Phytophthora palmivora of Soe mandarin in East Nusa Tenggara, Indonesia

Simamora,

Hahuly,

Kana

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Phytophthora palmivora-induced root and basal stem rot pose a significant threat to the survival of Soe mandarin plants in East Nusa Tenggara Province, Indonesia. We isolated four Trichoderma species, five Bacillus, and 13 Pseudomonas isolates from the rhizosphere of healthy Soe mandarin. This study pursued two main objectives: (a) assessing the inhibitory capabilities of indigenous Trichoderma spp., Bacillus, and Pseudomonas towards P. palmivora in vitro, and (b) investigating the combined efficacy of Trichoderma spp., Bacillus, and Pseudomonas in controlling P. palmivora in a pot trial. Results revealed that four Trichoderma species (T. asperellum, T. hamatum, T. harzianum, and T. viride) significantly inhibited the growth of P. palmivora. Notably, T. asperellum exhibited the highest inhibition, at 84.31%, followed closely by T. harzianum (84.11%), T. viride (83.67%), and T. hamatum (80.28%). Bacillus and Pseudomonas exhibited varying levels of inhibition to P. palmivora growth in vitro, with the most substantial inhibition observed in Bacillus 1, followed by Pseudomonas 6. In the subsequent pot trial, the application of Trichoderma, Bacillus, or Pseudomonas, either individually or in combination, significantly increased the height increment and reduced the disease incidence caused by P. palmivora in Soe mandarin seedlings.

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Frenemies of the soil: Bacillus and Pseudomonas interspecies interactions

Cited by 32 publications

References 99 publications

Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas

Exploring the antagonist potential of indigenous Trichoderma spp., Bacillus, and Pseudomonas against Phytophthora palmivora of Soe mandarin in East Nusa Tenggara, Indonesia

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