1994
DOI: 10.1099/13500872-140-5-1119
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Frequencies of lipopolysaccharide core types in Escherichia coli strains from bacteraemic patients

Abstract: We have investigated the distribution of the various core types (Rl, R2, R3, in 138 Escherichia coli isolates obtained from positive blood cultures. Rabbit antisera, raised against five rough strains expressing the respective core types, were made monospecific by extensive absorption. The reactivity of the antisera was tested in ELISA with bacterial cells that had been autoclaved for full exposure of core epitopes. One hundred and thirty strains could be typed directly, while eight strains required prior diges… Show more

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Cited by 64 publications
(78 citation statements)
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“…6 % of the isolates, respectively. However, the results presented by Appelmelk et al (1994) and those presented by Amor et al (2000), although representative of the whole E. coli species, may not accurately reflect the distribution of core types within commensal flora. Subtle relationships between LPS core type and the virulence determinants required to allow an organism to survive in a particular niche may affect the results.…”
Section: Results and Discussion Lps Core Type Detection By Pcr And Docontrasting
confidence: 45%
See 1 more Smart Citation
“…6 % of the isolates, respectively. However, the results presented by Appelmelk et al (1994) and those presented by Amor et al (2000), although representative of the whole E. coli species, may not accurately reflect the distribution of core types within commensal flora. Subtle relationships between LPS core type and the virulence determinants required to allow an organism to survive in a particular niche may affect the results.…”
Section: Results and Discussion Lps Core Type Detection By Pcr And Docontrasting
confidence: 45%
“…Four were either R4 or K12 but were assigned no core type due to the lack of any anti-K12 or -R4 monoclonal antibodies. Appelmelk et al (1994) investigated the distribution of LPS core types among 68 isolates from positive blood cultures. R1 core LPS was most frequently detected.…”
Section: Results and Discussion Lps Core Type Detection By Pcr And Domentioning
confidence: 99%
“…The core oligosaccharide that links the lipid A to the O-polysaccharide can be of ®ve types, denoted R1±R4 and K12. In several surveys, R1 has been shown to be the most common core type in the LPS of E. coli strains, with the R3 type being uncommon [8,9]. Although not based upon an exhaustive study, our experience indicates that the R3 core is more common in the LPS of diarrhoeagenic strains of E. coli, including many enterotoxigenic and enteropathogenic E. coli (ETEC and EPEC) strains and some shigellae, than in normal faecal isolates of E. coli.…”
Section: Introductionmentioning
confidence: 83%
“…The R1 core OS structure is the most prevalent among clinical isolates of E. coli (7,8) and, along with the R4 core OS structure, is also found in Shigella spp. The assignment of unambiguous glycosyltransferase activity to particular genes in E. coli K-12 and S. enterica sv.…”
Section: Lipopolysaccharide (Lps)mentioning
confidence: 99%
“…Whether strains containing R1-or R4-type LPS lack a second form of LPS is not known, and the biological impact of these differences is unclear. It is intriguing to note, however, that strains displaying an R1-type core predominate in clinical E. coli (7,8), and both R1 and R4 core types occur in Shigella spp. (33).…”
Section: The Waax Gene Of E Coli F2513 Encodes a Udp-galactose: (Glumentioning
confidence: 99%