The Eocene-Oligocene transition marks a fundamental step in the evolution of the modern climate. This climate change and the consequent major oceanic reorganisation affected the global carbon cycle, whose dynamics across this crucial interval are far from being clearly understood. In this work, the upper Eocene to lower Oligocene δ 13 C Carb and δ 13 C TOC records of a shallow-water and a hemipelagic carbonate settings within the Central Mediterranean area have been studied and discussed. The shallow-water carbon isotope signal has been analysed in the northern portion of the Apula Platform, cropping out in the Majella Mountain, Central Apennines (Santo Spirito Formation). A coeval Umbria-Marche basinal succession has been investigated in the Massignano section (Conero area, Central Italy). The purposes of this work are: to discriminate between the global and the local (Mediterranean) signature of C-isotope record during the Oi-1 event, to correlate the regional C-isotope signal with the global record, and to evaluate the carbon cycle dynamics across the greenhouse-icehouse transition through the integration of complementary records (shallow-water vs pelagic settings, δ 13 C Carb vs δ 13 C TOC). The upper Eocene carbon isotope record of the analysed successions matches with the global signal. The overall trend shows a decrease of the δ 13 C Carb and a contemporary increase of the δ 13 C TOC. The decoupling of the two curves is consistent with a reduced fractionation effect by primary producers that characterised the interval between the Middle Eocene Climatic Optimum and the onset of the Oi-1 event. However, regional factors superimposed the global signal. In fact, the upper Eocene basinal δ 13 C TOC record is marked by short-term negative spikes, which possibly represent times of higher productivity triggered by the westward subtropical Eocene Neotethys current entering from the Arabian-Eurasian gateway. On the contrary, the shallow-water record does not display these short-term productivity pulses. A change in the carbonate factory is only recorded at the Eocene-Oligocene transition, marked by a reduction of the larger benthic foraminifera and the spread of seagrass and corals. Moreover, in the shallow-water record of the Santo Spirito Formation, no major carbon isotope shift related to the Oi-1 event is recorded due to the presence of extensive slumps that disrupt the bedding. These slumps are the main evidence of the sea-level drop that occurred concomitantly with the onset of the Antarctica ice-sheet, which caused the deepening of the storm wave base and increased the instability over the entire ramp.