Frequency and Antimicrobial Resistance of Salmonella Serotypes on Beef Carcasses at Small Abattoirs in Jalisco State, Mexico

Abstract

<jats:p>The prevalence and antimicrobial resistance of Salmonella serotypes on beef carcasses from four small abattoirs in Jalisco State, Mexico, were investigated during a 10-month period. Following U.S. Department of Agriculture Food Safety and Inspection Service protocols, Salmonella was isolated from 78 (15.4%) beef carcasses (n = 505) after the final carcass water wash. Isolation frequency differed by establishment (P &amp;lt; 0.05) and was higher (P &amp;lt; 0.05) during the wet season (May through September) for all establishments. Thirteen Salmonella serotypes and four serogroups (partially serotyped isolates) were identified. The most prevalent were Salmonella enterica Give (24.4%), Salmonella Typhimurium (17.9%), and Salmonella Group B (14.1%). Antimicrobial susceptibility was tested against 11 drugs, and results indicated that 46.2% of the isolates were resistant to tetracycline, 42.3% were resistant to streptomycin, 23.1% were resistant to chloramphenicol, 21.8% were resistant to trimethoprim-sulfamethoxazole, and 19.2% were resistant to gentamicin. No resistance to ceftriaxone or ciprofloxacin was observed, and 33% of the isolates were resistant to three or more antimicrobials. Although Salmonella Give was the most prevalent serotype, 95% of the isolates of this serotype were susceptible to all antimicrobials tested. Antimicrobial resistance was more common in Salmonella Typhimurium, and 93% (13 of 14) of the isolates of this serotype were resistant to at least five antimicrobials. The frequency of multidrug-resistant Salmonella isolates differed among establishments (P &amp;lt; 0.05) and may be related to the origin of the cattle presented for harvesting. These findings highlight the need for control measures to reduce Salmonella prevalence on beef carcasses in small abattoirs in Mexico and for strategies to ensure the cautious use of antimicrobials in animal production to prevent and control the spread of antimicrobial-resistant foodborne pathogens.</jats:p>

Quantitative distribution of Salmonella spp. and Escherichia coli on beef carcasses and raw beef at retail establishments

“…The pathogen was detected in 18 ± 6% (26/142) of beef carcasses, 39 ± 10% (33/84) of beef chunks, and 71 ± 11% (46/65) of ground beef samples (Table 1). These results are in accordance with those previously reported for the same area of Mexico, where Salmonella was isolated from 15.4% (78/505) of beef carcass samples collected at non-federally inspected small abattoirs (Perez-Montano et al, 2012), and from 56.7% (135/238) of ground beef samples collected from 238 butcher's shops (Cabrera-Diaz et al, 2013). Likewise, none of the three abattoirs included in the present investigation were federally inspected and lacked the implementation of a food safety system.…”

Genetic diversity and antimicrobial resistance ofSalmonellaserotypes recovered throughout the beef production chain and from patients with salmonellosis

“…However, in countries like Turkey and Senegal, the pathogen was isolated from 21.3 and 87.4% of ground beef samples analyzed ( Arslan & Eyi, 2010 ; Stevens et al, 2006 ). Similarly, high frequencies of Salmonella in raw meats has been reported in Mexico; the pathogen was isolated from 6.4 to 28.6% of beef carcass samples collected at small non-federally inspected abattoirs ( Perez-Montano et al, 2012 ), from 29.9% of raw beef samples collected at retail, and from 56.7 to 71% of ground beef samples obtained from butcher’s shops ( Cabrera-Diaz et al, 2013 ; Martinez-Chavez et al, 2015 ).…”

Food Consumer Behavior and Salmonella Exposure Self‐Perception in the Central Region of Mexico

“…The consumer perception of high-risk food categories is in agreement with the high Salmonella spp. prevalence observed in raw meat products such as pork (13.3% to 58.1%), chicken (4.5% to 63.2%), and beef (6.1% to 54%) in Mexico (Cabrera-Diaz et al, 2013;Charles-Hernández et al, 2007;Miranda et al, 2009;Nayarit-Ballesteros et al, 2016;Perez-Montaño et al, 2012;Zaidi et al, 2006;Rodríguez-Ceniceros, Gómez-Hernández, & Vázquez-Sandoval, 2016;Villalpando-Guzmán et al, 2017).…”