Frequency and spontaneous cytotoxicity of natural killer cells in healthy children: preliminary results

Popko, Katarzyna; Malinowska, Iwona; Rychta, Marta; Osińska, Iwona; Górska, Elżbieta; Kucharská, Anna; Demkow, Urszula

doi:10.5114/ceji.2013.39776

Cited by 2 publications

(1 citation statement)

References 46 publications

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“…We did not notice such an influence in our study, but an impact of these substances on the number of NK cells after isolation in the total population of PBMC cannot be excluded. A correlation between the percentage of NK cells in circulation and results of the cytotoxicity test was previously found [ 19 ]. The separation technique used in our study (with Ficoll Sigma-Aldrich) can remove not only granulocytes to acquire PBMC but also dead lymphoid cells from the specimen (including NK cells), which may have contributed to decreased cytotoxicity after 24 hours [ 15 ].…”

Section: Discussionmentioning

confidence: 82%

Influence of blood sample storage and different types of anticoagulants on results of NK cytotoxicity tests

Popko,

Paskudzka,

Pieśniewska

et al. 2023

cejoi

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Introduction Natural killer (NK) cells are important players in the human immune response. Impaired NK function may lead to serious, life-threatening conditions. Defects may be consequences of genetic mutations or results of secondary factors such as infections, malignancies and autoimmune diseases. The cytotoxicity test is very useful, but its accessibility is limited to special immunological laboratories. Blood samples are often transported to remote centers, which takes time and requires special conditions. The aim of this study was to compare cytotoxicity assay results between samples preserved with three different anticoagulants to standardize the diagnostic procedure. Material and methods Peripheral blood from healthy donors was taken with three anticoagulants: heparin, K 2 EDTA and citrate. Peripheral blood mononuclear cells (PBMC) were isolated and tested directly after blood drawing and after 24-hour storage. Cytotoxic abilities of NK cells were tested in 4 h co-culture with K562. NK cytotoxicity was measured by flow cytometry. Results In most cases of analyzed healthy donors, cytotoxicity results were similar regardless of type of anticoagulant. However, the highest mean values were obtained in samples with citrate. There was a significant decrease in cytotoxicity after 24 hours of storage of the whole blood at ambient temperature. The mean drop in cytotoxicity results was substantial for all anticoagulants: 76% for heparin, 67% for citrate and 70% for EDTA. Conclusions Results of spontaneous NK cytotoxicity seem to be affected by the anticoagulants used for blood protection. Commercial instant cytotoxicity testing and delayed analysis after blood storage gave the highest results in blood with sodium citrate.

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Section: Discussionmentioning

confidence: 82%

Influence of blood sample storage and different types of anticoagulants on results of NK cytotoxicity tests

Popko,

Paskudzka,

Pieśniewska

et al. 2023

cejoi

View full text Add to dashboard Cite

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Disturbances in NK Cells in Various Types of Hemophagocytic Lymphohistiocytosis in a Population of Polish Children

Popko¹,

Górska²,

Wołowiec

et al. 2019

Journal of Pediatric Hematology/Oncology

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Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening disease associated with immune system hyperactivation and the appearance of serious systemic disturbances. The purpose of this study was an assessment of natural killer (NK) cell disturbances in a group of children with clinical signs of HLH. A total of 43 children with HLH and 17 healthy children were enrolled in the study. NK phenotyping, intracellular perforin staining, and cytotoxicity tests were performed by using the flow cytometry method. HLH patients were divided into 6 HLH types: 9% infection-related HLH; 7% malignancy-related HLH; 21% macrophage activating syndrome; 12% familial hemophagocytic lymphohistiocytosis; 2% X-linked lymphoproliferative syndrome; and 49% as HLH of unknown background. A positive correlation was observed between cytotoxicity and NK cells in children with HLH (P=0.01). In all HLH groups, the percentage of NK cells was significantly lower than in the control population. The spontaneous cytotoxicity was significantly lower in HLH patients. The results presented in this study indicate the importance of impaired function and the number of NK cells in the pathogenesis of HLH. Nonetheless, the background of disturbances seems to be different in various cases.

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Frequency and spontaneous cytotoxicity of natural killer cells in healthy children: preliminary results

Cited by 2 publications

References 46 publications

Influence of blood sample storage and different types of anticoagulants on results of NK cytotoxicity tests

Influence of blood sample storage and different types of anticoagulants on results of NK cytotoxicity tests

Disturbances in NK Cells in Various Types of Hemophagocytic Lymphohistiocytosis in a Population of Polish Children

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