Frequency detection of imidacloprid resistance allele in Aphis gossypii field populations by real-time PCR amplification of specific-allele (rtPASA)

Zhang, Jing; Cui, Li; Xu, Xin; Rui, Changhui

doi:10.1016/j.pestbp.2015.06.001

Cited by 19 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The R81T mutation was reported previously in the cotton aphids sampled from different provinces of China (Chen et al, 2017;Zhang et al, 2015). This mutation was not observed in samples from Shawan of Xinjiang collected in 2014 (Chen et al, 2017), but detected in our Xinjiang samples collected in 2019.…”

Section: Discussionsupporting

confidence: 48%

“…The emergency of the R81T mutation in Xinjiang may be a result of continued application of neonicotinoids in this region. Although the exact effect of R81T mutation in the cotton aphid remains unclear, previous studies indicated that R81T mutation may occur in the strains with an imidacloprid resistance ratio >10-fold (Chen et al, 2017;Koo et al, 2014;Zhang et al, 2015). The role of R81T in neonicotinoid resistance was recently validated by using CRISPR/Cas9 introduced R81T mutation in the genome of Drosophila melanogaster (Homem et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…imidacloprid) resistance has been attributed to mutations in nAChR, reduced expression of nAChR or increased rates of insecticide detoxification (Chen et al, 2017). A point mutation (R81T) in the loop D region of the nAChR β1 subunit has been identified to be associated with imidacloprid resistance in both Myzus persicae (Bass et al, 2011) and A. gossypii (Chen et al, 2017;Hirata et al, 2015Hirata et al, , 2017Koo et al, 2014;Shi et al, 2012;Zhang et al, 2015), and this mutation has been detected in laboratory-selected A. gossypii strains and in field-resistant populations (Chen et al, 2017;Hirata et al, 2015;Koo et al, 2014;Shi et al, 2012;Zhang et al, 2015). The causal link between neonicotinoid resistance and the R81T mutation has been supported by the fact that R81 within loop D is a key determinant of neonicotinoid binding to nAChRs (Ihara et al, 2008;Shimomura et al, 2006), and by electrophysiological analysis (Hirata et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

First report of voltage‐gated sodium channel M918V and molecular diagnostics of nicotinic acetylcholine receptor R81T in the cotton aphid

Munkhbayar

Liu

et al. 2020

J Applied Entomology

View full text Add to dashboard Cite

The cotton aphid, Aphis gossypii Glover, is one of the most important agricultural insect pests. Pyrethroid and neonicotinoid insecticides have generally shown excellent control of A. gossypii, but many populations of this pest have developed resistance against these classes of insecticides. The success of insecticide resistance management strategies requires detailed knowledge of both phenotype and genotype of the target insect pest. In this study, we attempted to understand the molecular status of insecticide resistance in cotton aphid populations in Xinjiang Uygur Autonomous Region of China, the major cotton planting region of China. In addition to the previously reported M918L mutation, we discovered another substitution (M918V) in the voltage-gated sodium channel (VGSC). Moreover, we developed a molecular assay that could be used to detect precisely the R81T mutation in the nicotinic acetylcholine receptor (nAChR). This survey revealed that 918L was the predominant VGSC allele with a frequency ranging from 50.0% to 56.7%. Notably, appreciable frequencies (between 10% and 40%) of the resistance 81T allele of the nAChR gene were detected in three investigated populations. The prevalent co-occurrence of both VGSC 918L/V and nAchR 81T indicates a worrisome situation of multiple resistance to both pyrethroids and neonicotinoids.

show abstract

Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First report of voltage‐gated sodium channel M918V and molecular diagnostics of nicotinic acetylcholine receptor R81T in the cotton aphid

Munkhbayar

Liu

et al. 2020

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…In China, A . gossypii was also reported to be resistant to imidacloprid 6 , 7 . Therefore, new insecticides should be introduced to control resistant A .…”

Section: Introductionmentioning

confidence: 99%

Sublethal effects of the novel cis-nitromethylene neonicotinoid cycloxaprid on the cotton aphid Aphis gossypii Glover (Hemiptera: Aphididae)

Cui

Yuan

Wang

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Cycloxaprid is a novel cis-configuration neonicotinoid insecticide that is effective against a wide range of insect pests, including those that are resistant to conventional neonicotinoids. In this study, life table parameters were applied to estimate the cycloxaprid-induced sublethal effects on Aphis gossypii. The results indicated that the LC20 (0.81 mg a.i. L−1) of cycloxaprid significantly decreased the pre-oviposition period in first-progeny adults. Additionally, the life expectancy of F1 generation adults was reduced. However, no significant differences were observed for the intrinsic rate of increase (ri), finite rate of increase (λ), net reproductive rate (R0), or mean generation time (T) of F1 individuals. Therefore, resurgence in the A. gossypii population induced by a low concentration of cycloxaprid might not occur. Additionally, the response of the detoxification enzymes showed that cycloxaprid at the LC20 inhibited cytochrome P450 monooxygenase (P450) and glutathione S-transferase (GST) activities at 6 h after exposure. Such inhibition of P450 and GST activities could lead to a decrease in the metabolism of cycloxaprid, which would increase the efficacy of cycloxaprid. Therefore, our results contribute to the assessment of the overall effects of cycloxaprid on A. gossypii.

show abstract

“…However, extensive application of these insecticides over the past decades has led to the development of pesticide resistance in these cotton aphids. 9) The increased insecticide applications in attempts to control these resistant strains pose substantial environmental risks, including toxicity to nontarget organisms such as natural predators. [10][11][12] Predatory natural enemies play important roles in integrated pest management (IPM).…”

Section: Introductionmentioning

confidence: 99%

Effects of exposure to imidacloprid direct and poisoned cotton aphids <i>Aphis gossypii</i> on ladybird <i>Hippodamia variegata</i> feeding behavior

Wumuerhan

YunTao

2020

J. Pestic. Sci.

View full text Add to dashboard Cite

Imidacloprid is a pesticide used to control aphid infestations of cotton plants. However, poisoned aphids also serve as food for the ladybird natural predator Hippodamia variegata. We investigated whether imidacloprid-treated eggs, pupae, and adults of H. variegata and poisoned aphids altered ladybird predatory behavior. Laboratory bioassay results demonstrated that 0.72 g/L imidacloprid was lethal to ladybirds. Imidacloprid significantly reduced the hatching and emergence rates of H. variegata, and these effects were time and dose dependent. Predation was most adversely affected when the ladybirds directly consumed poisoned aphids and less so when directly exposed to the insecticide at sublethal concentrations. Imidacloprid use in cotton fields should be restricted to the initial stages of aphid infestation to avoid the period when adult ladybirds are present.

show abstract

Frequency detection of imidacloprid resistance allele in Aphis gossypii field populations by real-time PCR amplification of specific-allele (rtPASA)

Cited by 19 publications

References 25 publications

First report of voltage‐gated sodium channel M918V and molecular diagnostics of nicotinic acetylcholine receptor R81T in the cotton aphid

First report of voltage‐gated sodium channel M918V and molecular diagnostics of nicotinic acetylcholine receptor R81T in the cotton aphid

Sublethal effects of the novel cis-nitromethylene neonicotinoid cycloxaprid on the cotton aphid Aphis gossypii Glover (Hemiptera: Aphididae)

Effects of exposure to imidacloprid direct and poisoned cotton aphids <i>Aphis gossypii</i> on ladybird <i>Hippodamia variegata</i> feeding behavior

Contact Info

Product

Resources

About