Frequency Distribution of IL-17A G197A (rs2275913) and IL-17F A7488G (rs763780) Polymorphisms Among Healthy Sudanese Population

Mohamed, Nouh Saad; Siddig, Emmanuel Edwar; Ahmed, Abdallah E.; Albsheer, Musab MA.; Abdelbagi, Hanadi; Ali, Eman; Alsubki, Anadel A.; Abdalaziz, Sabah A.; Mustafa, Madinna; Muneer, Mohamed S.; Osman, Hussam A.; Osman, Maha M.; Ali, Mohamed S.; Edris, Ali MM.; Ahmed, Ayman; Omer, Rihab Ali

doi:10.21203/rs.3.rs-31741/v1

Cited by 2 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20 Although the sample size was not determined before conducting the study, the significant distribution of IL-17A genotypes among those with a family history of RA reported in this study could be attributed to the role of IL-17A SNPs inheritance to the descendants. 6,20…”

Section: Discussionmentioning

confidence: 78%

“…26 Also, as previously, the distribution of IL-17A genotype AA was significant among the Arab ethnic group. 20 Although the sample size was not determined before conducting the study, the significant distribution of IL-17A genotypes among those with a family history of RA reported in this study could be attributed to the role of IL-17A SNPs inheritance to the descendants. 6,20 The abnormal values of the clinical examinations including RF, CRP, anti-CCP, and ESR are the most features to determine RA.…”

Section: Discussionmentioning

confidence: 78%

“… 24 Previously, investigation of IL-17A polymorphisms among the Sudanese population to determine the frequency distribution of the different IL-17A genotypes was conducted among healthy individuals. 20 In this study, we investigated the association of IL-17 rs2275913 polymorphism with RA susceptibility and outcome in the Sudanese population. The most frequent affected gender among the Sudanese population was found to be the female gender.…”

Section: Discussionmentioning

confidence: 99%

“…IL-17A genotyping made using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technique. The primers used for amplifying the IL-17A gene previously published by Mohamed et al, 20 PCR conditions were applied in a PCR thermocycler (MJ Research, USA). PCR conditions were consisted of a pre-denaturation step of 4 min at 94°C, followed by 40 cycles of 30 s denaturation at 94°C, 30 s annealing at 55°C, and 30 s elongation at 72°C.…”

Section: Methodsmentioning

confidence: 99%

“… 19 In the IL-17 gene, there were several polymorphisms detected that might influence the expression of IL-17 . 20 , 21 Not only the IL-17A - 197G/A (rs2275913) was associated with the risk to RA, but also the severity of the disease and the response to treatment. 22 , 23 Therefore, this study aimed to investigate the association between IL-17A-197G/A (rs2275913) single nucleotide polymorphism and RA among Sudanese RA patients.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Association of interleukin-17A rs2275913 polymorphism with rheumatoid arthritis susceptibility in Sudanese population

et al. 2021

Self Cite

View full text Add to dashboard Cite

Objectives: Rheumatoid arthritis is a chronic inflammatory autoimmune disease. This study aimed to determine the association of interleukin-17A-197G/A polymorphism with rheumatoid arthritis in Sudanese patients. Methods: A case–control study was conducted between March and December 2018. Clinical and demographic data of the study participants were collected and analyzed. Polymerase chain reaction restriction fragment length polymorphism molecular technique was done to investigate interleukin-17A-197G/A polymorphisms. All statistical tests were considered statistically significant when p < 0.05. Results: The study population included 266 participants aged between 1 and 85 years, with an average of 40 years, classified into 85 (31.2%) cases (mean age 48.5 ± 11.3 years), and 181 (68.8%) controls (mean age 35.3 ± 15.9 years). The interleukin-17A homozygote AA genotype was more frequent among the control group compared to the case group; 95 (52.5%) and 7 (8.2%), respectively. The homozygote GG and the heterozygote AG genotypes were proportionally not different among the cases and control groups; 13 (54.2%) and 11 (45.8%), and 65 (46.4%) and 75 (53.6%), respectively. According to the distribution of interleukin-17A genotypes, a statistically significant difference was observed among cases with the interleukin-17A AA and AG genotypes, p values 0.001 and 0.004, respectively. For the association interleukin-17A genotypes and family history a negatively significant association was reported (95% confidence interval, –0.219, p value = 0.001). There was also a negatively significant association of interleukin-17A genotypes and anti-cyclic citrullinated peptide (95% confidence interval, −0.141, p value = 0.002). Conclusion: This study is the first study in Sudan established the association between interleukin-17A-197G/A (rs2275913) polymorphisms and susceptibly to rheumatoid arthritis. These findings appeal for further research in Sudan to investigate the exact role of IL-17A in immunopathology and disease severity among Sudanese rheumatoid arthritis

show abstract

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Association of interleukin-17A rs2275913 polymorphism with rheumatoid arthritis susceptibility in Sudanese population

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Knowledge and attitudes towards cervical cancer prevention among women in Khartoum state, Sudan

Siddig

Ahmed

et al. 2023

Womens Health (Lond Engl)

Self Cite

View full text Add to dashboard Cite

Background: Cervical cancer is considered the third leading cause of death among women worldwide, and human papillomavirus was identified as a major causative agent for developing cervical cancer. Objectives: This study aimed to assess the knowledge and attitudes towards cervical cancer prevention among women in Khartoum state, Sudan. Design: A community-based cross-sectional study implemented in Khartoum state, Sudan, from 1 August 2020 to 1 September 2020. Methods: We conducted a descriptive cross-sectional community-based study using an electronic questionnaire for data collection. Descriptive statistics, frequency, mean, and percentage were computed. Results: The study included 716 female participants with a mean age of 27.6 + 8.7 years. 580 (81.0%) and 229 (32.0%) had heard about cervical cancer and Pap test, respectively. cervical cancer was assumed related to alcohol consumption 109 (15.2%), giving birth to many children 51 (7.1%), ageing 118 (16.5%), and having many sexual partners 335 (46.8%). In addition, 300 (41.9%) attributed cervical cancer to having human papillomavirus infection, 256 (35.6%) to the prolonged use of contraceptives, and 162 (22.6%) to smoking. Knowledge about the best time to be vaccinated against human papillomavirus, 110 (15.4%) stated it is better after marriage. Regression models to predict the effectors on participants’ knowledge and attitudes showed a low standard deviation of the estimates with higher values of the adjusted R2 [R: 0.041, 0.017, and 0.006; std: 1.527, 0.417, and 0.426]. This indicates the combined influence of occupation, educational level, family income, and marital status on the participant’s knowledge and attitude levels. Conclusion: This study revealed that the participant’s knowledge and attitudes levels are mainly driven by their occupation, educational level, family income, and marital status altogether. This underscores the need for a countrywide community engagement campaign through health education and raising awareness sessions, and massive social media to sensitize the community and healthcare providers about the risk of cervical cancer and the available prevention and control measures.

show abstract

Frequency Distribution of IL-17A G197A (rs2275913) and IL-17F A7488G (rs763780) Polymorphisms Among Healthy Sudanese Population

Cited by 2 publications

References 20 publications

Association of interleukin-17A rs2275913 polymorphism with rheumatoid arthritis susceptibility in Sudanese population

Association of interleukin-17A rs2275913 polymorphism with rheumatoid arthritis susceptibility in Sudanese population

Knowledge and attitudes towards cervical cancer prevention among women in Khartoum state, Sudan

Contact Info

Product

Resources

About