As an important part of many mechanical equipment, the mechanical transmission system is very important to carry out efficient and accurate fault monitoring and diagnosis. Compared with traditional fault diagnosis techniques, such as spectrum analysis, deep learning has been widely used in the field of mechanical system fault diagnosis due to its powerful data expression ability, and has achieved certain research results. One-dimensional convolutional neural network is a widely used model for deep learning, so in this paper, the one-dimensional convolutional neural network (1D-CNN) in the deep learning theory and the vibration signal analysis method are integrated and applied to the fault identification of mechanical transmission system to achieve accurate diagnosis and classification of faults. The experiment is mainly to collect the vibration signal data of different fault states such as broken teeth, cracking, shaft unbalance, bearing wear, and excessive friction of the driven wheel of the mechanical transmission system, it was divided into training set and testing set according to an appropriate proportion, and 1D-CNN was built using Python. The deep learning model deeply analyzed the influence of different data sample sizes and different model parameters on the recognition accuracy, and obtained an ideal diagnostic model based on variable learning rate through parameter adjustment and comparative analysis. This experimental results show that the recognition method based on one-dimensional convolutional neural network can be effectively applied to the fault diagnosis of related mechanical transmission, and has a high diagnosis accuracy.