Frequency of antibodies to Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia burdgorferi in cattle from the northeastern region of the state of Pará, Brazil

Abstract

Babesiosis, anaplasmosis, and trypanosomosis are relevant diseases, potentially causing morbidity in cattle, leading to economic losses. Borreliosis is import as a potential zoonosis. The objective of this study was to determine, by indirect enzyme-linked immunosorbent assay (ELISA), the frequency of seropositive cattle to Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia burgdorferi in cattle from the Northeastern region of Pará, Brazil. Sera samples from 246 female adult cattle from municipalities of Castanhal and São Miguel do Guamá were used. Crude antigens ELISAs were used to detect antibodies to all agents, except to A. marginale, to which an indirect ELISA with recombinant major surface 1a protein (MSP1a) antigen was used. Overall frequencies of seropositive animals were: B. bigemina -99.2%; B. bovis -98.8%; A. marginale -68.3%; T. vivax -93.1% and B. burgdorferi -54.9%. The frequencies of seropositive cattle to B. bovis and B. bigemina suggest a high rate of transmission of these organisms by tick in the studied region, which can be classified as enzootically stable to these hemoprotozoans. The low frequency of seropositive cattle to A. marginale may be attributed to a lower sensitivity of the recombinant antigen ELISA utilized or a distinct rate of inoculation of this rickettsia by ticks, as compared with Babesia sp. transmission. The high frequency of seropositive cattle to T. vivax indicates that this hemoprotozoan is prevalent in herds from the Northeastern region of Pará. The rate of animal that showed homologues antibodies to B. burgdorferi indicates the presence of the tickborne spirochaetal agent in the cattle population in the studied region.

Serological detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central-western regions of Brazil

“…The frequency of positive tests for B. bovis from the three states addressed in this study is consistent among the states as well as with studies performed in different regions of Brazil (BARROS et al, 2005;D'ANDREA et al, 2006;GUEDES-JUNIOR et al, 2008;TRINDADE et al, 2010). The prevalence of B. bigemina and A. marginale observed at the state level in Tocantins was lower than the prevalence observed in the Araguaína region of Tocantins according to Trindade et al (2010) (99.2%) and Trindade et al (2011) (89.9%).…”

Ocorrência sorológica de Babesia bovis, Babesia bigemina e Anaplasma marginale em bovinos e bubalinos no estado do Pará, Brasil

“…A ocorrência sorológica para os três agentes em bovinos observada no presente estudo corrobora com os resultados encontrados na região nordeste do estado do Pará por Guedes Junior et al (2008). Em um estudo realizado no semi-árido da Bahia a ocorrência de anticorpos anti-B.…”

Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

“…A prevalência sorológica e molecular de Babesia bovis e Babesia bigemina observadas neste estudo foi inferior aos resultados encontrados por Guedes et al (2008) no Brasil e superiores aos encontrados por Terkawi et al (2011) na Thailândia e por He et al (2012) He et al (2012).…”