Frequency of Bacetrial Content Finding in Persistant Periapical Lesions

Abstract

ObjectivesTo determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and diabetes mellitus.Materials and methodsThe samples of persistent apical lesions were collected during apicoectomy. Bacterial ubiquitous primer 16S rRNA was used to detect 16S ribosomal RNA in 36 samples. A species–specific PCR was performed with primers targeted to the bacterial 16S rRNA genes of Prevotella Nigrescens, Pseudoramibacter alactolyticus, and Propionobacterium propionicum.ResultsSix samples (16.67%) were positive for bacterial ribosomal RNA. Pseudoramibacter alactolyticus was detected in three samples. Propionibacterium propionicum and Prevotella nigrescens were detected in one sample each. The prevalence of infection of such lesions with P. intermedia, P. propionicum and P. alactolyticus is low.ConslusionThe study we conducted gave insufficient data about extraradicular infection and its connection with diabetes mellitus and clinical symptoms.ConclusionsApical lesions persisting after endodontic treatment could harbor microorganisms other than Actinomyces and Propionicum species.

Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment

“…Actinomyces naeslundii, S. sobrinus, and G. morbillorum were not detected in the present study and have not been investigated by several researches (Geibel et al 2005, Zhang et al 2010, Grgurevi c et al 2017, Gomes et al 2017. However, Actinomyces species are often found in periapical lesions in the format of aggregates that look like 'starbursts' when examined histologically (Siqueira, 2003).…”

“…A marginal incision was chosen because it does not provoke scars compared to submarginal incision (Kim & Kratchman 2006). Indeed, it was previously noted that the risk of sample contamination using marginal incision was not significantly different from a submarginal incision (Sunde et al 2000b, Grgurevi c et al 2017. Nonetheless, when the microbiota of periapical lesion was compared to the mucosa and the exposed bone surface, it was apparent that most of the bacteria detected in periapical lesions were different from other sites (Sunde et al 2000a).…”

Inspection of the Microbiota in Endodontic Lesions

“…In cases where chronic apical periodontitis persists even after re-treatment, even if performed correctly, one might not consider persistent intra-radicular infection [70], but rather, persistent apical extra-radicular infection [71,72]. Such infections are no longer etiologically sustained by bacteria, but by Actinomycetes such as Actinomyces israelii (the most frequent) [73], and in some cases, Propionibacterium propionicum [74].…”

Historical and Contemporary Perspectives on the Microbiological Aspects of Endodontics

“…Presently, with the understanding of biofilms and their development in the apical regions and expansion on to the apical cementum, essentially the presence of apical periodontitis is the extension of pulpal disease and root canal infections with vast array of organisms, many of which are well-known and fastidious, and many of which are yet to be identified. The predominant organisms present have been identified as obligate anaerobes [121,143,144,145,146,147,148,149,150,151], such as;…”