Frequency of BCL-2/JH translocation in healthy males exposed to low-level radiation in comparison to age-matched healthy controls

Dölken, Lars; Schüler, Frank; Dölken, Gottfried

doi:10.1182/blood-2002-03-0887

Cited by 13 publications

(9 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The correlation between low levels of ionizing radiation and the new malignant event in the B-rearranged cells remains undefined (Dölken et al, 2002). Our study showed that radio-exposed individuals presented low levels of cells bearing the BCL2/JH rearrangement; the lower incidence of this rearrangement in healthy individuals compared with that observed in other studies (Schüler et al, 2003;Mahfouz et al, 2007) likely reflects the smaller amount of DNA analyzed for each individual.…”

Section: Discussioncontrasting

confidence: 46%

“…Although low levels of radiation as described in other studies (Dölken et al, 2002) have no significant relation to the increase of the t(14;18) cells and do not display evidence of being the causative event of other genetic mutations that culminate in neoplastic development, age, heavy smoking and exposure to pesticides were described as factors increasing the frequency of t(14;18) cells. However, for almost all subjects in this study, no significant relationship between the presence of t(14;18) and alcohol consumption or/and heavy smoking was expected.…”

Section: Discussionmentioning

confidence: 90%

“…In Japanese individuals, the frequency of the BCL2/J(H) translocation increased dramatically at around 20 years of age (Yasukawa et al, 2001). In addition, previous reports demonstrated that the frequency of the BCL2/J(H) translocation rises with age in white individuals (Dölken et al, 2002). This study is part of the constant genetic biomonitoring of individuals accidentally exposed to 137Cs in Goiânia and their offspring.…”

Section: Discussionmentioning

confidence: 99%

“…Based on this observation, studies were initiated to investigate whether exposure to low-level radiation could influence the frequency as well as the total number of circulating t(14;18)-positive cells in healthy individuals (Dölken et al, 2002), that is, the clonal expansion or progression as well the generation of the translocation.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Short Communication Assessment of BCL2/J(H) translocation in healthy individuals exposed to low-level radiation of 137CsCl in Goiânia, Goiás, Brazil

Nunes¹,

Laranjeira²,

Yunes³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Healthy radio-exposed individuals who received low levels of Cesium-137 radiation during the accident that occurred in Goiânia in 1987, their families and controls were tested for the detection of t(14;18)-rearranged B cells in peripheral blood by using a highly sensitive, real-time quantitative PCR method. The chromosomal translocation t(14;18)(q32;q21) is characteristic of follicular lymphoma and is a frequent abnormality observed in other types of non-Hodgkin's BCL2/J(H) translocation in exposed individualslymphoma. This translocation leads to constitutive activation of the BCL2 oncogene by the enhancers of the immunoglobulin heavy-chain locus. In healthy individuals, the same translocation may also be found in a small fraction of peripheral blood lymphocytes, and positive cells might serve as an indicator for environmental exposure to carcinogens and possibly correlate with the cumulative risk of developing t(14;18)-positive non-Hodgkin's lymphoma. Twenty healthy radio-exposed individuals, 10 relatives and 10 non-exposed healthy individuals were tested for the detection of this translocation. Only 1 non-exposed individual was positive for the chromosomal translocation, and healthy radio-exposed individuals presented lower levels of cells bearing the BCL2/J(H) rearrangement when compared to the levels of the patients with follicular lymphoma before treatment. However, evaluation of more cells would be required to confirm the total absence of circulating cells bearing BCL2/J(H) rearrangement.

show abstract

Section: Discussioncontrasting

confidence: 46%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Short Communication Assessment of BCL2/J(H) translocation in healthy individuals exposed to low-level radiation of 137CsCl in Goiânia, Goiás, Brazil

Nunes¹,

Laranjeira²,

Yunes³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Thus, we cannot exclude that part of the positive results reported here are derived from t(14;18) in non-malignant lymphocytes unrelated to follicular lymphoma. The majority of healthy individuals being positive for t (14;18) carry the translocation at a frequency below 10 −4 [18]. In another study with 25% of healthy volunteers being positive for t (14;18), only 3% were positive at frequencies of more than one in 10 4 cells [17].…”

Section: Discussionmentioning

confidence: 94%

Commercial LightCycler®-based quantitative real-time PCR compared to nested PCR for monitoring of Bcl-2/IgH rearrangement in patients with follicular lymphoma

Kornacker

Schmitt

et al. 2008

Ann Hematol

View full text Add to dashboard Cite

No evidence for simian virus 40 DNA sequences in malignant non-Hodgkin lymphomas

et al. 2006

View full text Add to dashboard Cite

DNA sequences coding for simian virus 40 (SV40) large T antigen have been detected at different frequencies in human non-Hodgkin's lymphomas (NHL) by PCR techniques as well as immunohistochemistry. A highly sensitive quantitative real-time PCR specific for a sequence of SV40 large T antigen was established to test whether SV40 DNA is present in malignant lymphomas of German patients. Thirty-three lymph node samples obtained from 27 patients with NHL and 6 patients with Hodgkin's disease (HD) were tested in addition to 48 samples of peripheral blood mononuclear cells (PBMNC) from patients with NHL containing between 0.1% and >90% circulating lymphoma cells determined by PCR. Fourteen lymph nodes obtained from patients with other diseases than malignant lymphomas and 47 PBMNC samples from healthy volunteers served as controls. All samples from patients with malignant lymphomas and all controls were negative for SV40 DNA by quantitative real-time. In contrast, EBV-DNA could be detected in 29 of 46 DNA preparations isolated from lymph nodes (63%) and in 20 of 47 DNA preparations from PBMNC. EBV-positive samples contained between 5 and 80,000 EBV copies per 100,000 cells. Our results do not support the hypothesis that SV40 plays a major role in the etiology of malignant lymphomas and, in addition, they exclude a clonal SV 40 infection of malignant lymphoma cells in all samples investigated. ' 2005 Wiley-Liss, Inc.Key words: SV40; malignant NHL; quantitative real-time PCR; EBV Simian virus 40 (SV40) is a small double stranded DNA virus of 5243 bp belonging to the polyomaviruses. The primary viral gene product is the SV40 large T antigen, a nuclear phosphoprotein with multiple functions essential for virus replication and transformation of rodent and human cells. [1][2][3] The large T antigen is encoded by 2 exons corresponding to nucleotides 2691-4571 and 4918-5163. In SV40 infected cells large T antigen forms a complex with nuclear proteins, such as p53 and pRb, thereby inactivating their function. 4 The oncogenicity of SV40 has been shown in experiments when the virus was injected into hamsters. Depending on the route of inoculation a variety of tumors were found including ependymomas, choroid plexus papillomas, brain tumors, osteosarcomas, soft tissue sarcomas mesotheliomas and lymphomas. 5 The natural hosts of SV40 are Asian macaques and infections of humans seem to occur only upon close contact with infected monkeys or their tissues. 6-8 Between 1955 and 1963 in the United States, Canada and Europe millions of children and adults below 40 years of age were accidentally exposed to the virus by SV40-contaminated poliovirus and adenovirus vaccines prepared from monkey kidney cells by receiving one or more doses of a vaccine contaminated with live virus. 6,9 During the following 20 years laboratories all over the world have reported the isolation of SV40 from cultured human tumor cells by fusion with permissive cells 10,11 or the presence of nucleic acid sequences homologous to SV40, in particular to the SV40 lar...

show abstract

Frequency of BCL-2/JH translocation in healthy males exposed to low-level radiation in comparison to age-matched healthy controls

Cited by 13 publications

References 7 publications

Short Communication Assessment of BCL2/J(H) translocation in healthy individuals exposed to low-level radiation of 137CsCl in Goiânia, Goiás, Brazil

Short Communication Assessment of BCL2/J(H) translocation in healthy individuals exposed to low-level radiation of 137CsCl in Goiânia, Goiás, Brazil

Commercial LightCycler®-based quantitative real-time PCR compared to nested PCR for monitoring of Bcl-2/IgH rearrangement in patients with follicular lymphoma

No evidence for simian virus 40 DNA sequences in malignant non-Hodgkin lymphomas

Contact Info

Product

Resources

About