Frequency of hepatitis B virus DNA in anti-HBc positive, HBsAg negative blood donors in Rasht, northern Iran

Khamesipour, Ali; Mohtasham-Amiri, Zahra; Amini-Kafiabad, Sedigheh; Saadat, Farshid; Ghanaei, F Mansour; Esteghamati, Abdoulreza; Shakib, Reza Jafari

doi:10.1016/j.transci.2011.08.005

Cited by 16 publications

(11 citation statements)

References 15 publications

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“…The real prevalence of occult HBV infection is still unknown (Khamesipour et al 2011). The data generated by various studies are not comparable mainly due to differences in the methodologies used; however, detection of HBV-DNA using a NAT method is the most sensitive test currently used, but there is no valid standard assay to detect occult HBV infection (Raimondo et al 2007;Raimondo et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Vaccine Reduced the Prevalence of Antibodies to Hepatitis B Core Antigen in Blood Donors in Aseer Region,Saudi Arabia

Ibrahim

Dajem

Heijan³

et al. 2014

Egyptian Academic Journal of Biological Sciences. C, Physiology

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Viral hepatitis is one of the major problem worldwide. The detection of hepatitis B virus (HBV) surface antigen (HBsAg), antibodies to HBV core antigen (HBc-Ab), and HBV-DNA in donated blood in Aseer Region (Southern part of Kingdom of Saudi Arabia) to detect prevention of the residual risk of transfusion transmitted HBV, donors in window period of HBV infection and the prevalence of HBV infection was attempted. The study was conducted on random blood samples collected from healthy blood donor volunteers, who were referred to blood transfusion centers found at Aseer region, during the period from March 2012 to January 2013. All the collected blood units were screened for hepatitis B surface antigen (HBsAg), anti-HBc, hepatitis C virus (HCV), human immunodeficiency virus (HIV) 1 and 2, human T-cell lymphotropic virus (HTLV) I/II, venereal disease research laboratory (VDRL), and malaria. All donated blood samples were checked for HBV-DNA, HCV-RNA, and HIV-RNA by nucleic acid test (NAT) technology. Of 6698 (19 females (0.28%) and 6679 males (99.72)) blood donors screened, with median age of 28 (female) and 30 years (males), 69 (1.03%) were HBsAg positive of them 64 (0.96) were positive to HBV-DNA, 411 (6.14%) were anti-HBc positive of them 73 (1.09%) were positive to HBV-DNA. Cases positive to both HBsAg and HBc-Ab were 68 (1.02%) of them 63 (0.94%) were positive to HBV-DNA. All HBsAg and HBc-Ab positive cases were shown to be among male volunteers. In conclusion, prevalence of HBsAg in Asser region is very low. The rate of HBc-Ab in units of blood donation is relatively high. The presence of HBV-DNA in HBc-Ab positive donations makes it risky for use. Vaccination program against HBV decreased the rate of HBV transmission.

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Section: Discussionmentioning

confidence: 99%

Hepatitis B Vaccine Reduced the Prevalence of Antibodies to Hepatitis B Core Antigen in Blood Donors in Aseer Region,Saudi Arabia

Ibrahim

Dajem

Heijan³

et al. 2014

Egyptian Academic Journal of Biological Sciences. C, Physiology

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“…HBV DNA was detected in 29.7% of HBcAb positive samples by Real Time PCR (18). In another study done in Rasht, the prevalence of HBcAb positive samples was 3.8% and only one case was positive for viral DNA (19). In Arak, Sofian et al .…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

et al. 2013

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BackgroundPresence of occult hepatitis B infection (OBI) renders HBs antigen (HBsAg) undetectable by ELISA. Therefore it is valuable to evaluate the frequency of OBI among healthy blood donors to improve and perhaps change the strategies of blood screening to reduce the risk of HBV transmission.ObjectivesThe aim of this study was to determine the presence of HBcAb and HBV DNA among Iranian HBsAg negative healthy blood donors who donated their blood to the Tehran Blood Transfusion Center during 2011.Patients and Methods1000 serum specimens negative for HBsAg, HCV antibody and HIV antibody were collected from healthy blood donors and tested for HBcAb. Presence of hepatitis B viral DNA was checked in HBcAb positive samples by nested PCR with two sets of primers to amplify part of HBV S gene.ResultsThere were 64 women and 936 men in the population under study. The mean ± SD age of the donors was 38 ± 11 years. 80 out of 1000 samples (8%) were found to be positive for HBcAb. HBV DNA was detected in 50% of HBcAb positive specimens. The mean ± SD age of donors without HBV DNA was 37.7 ± 10.5 years and for donors with HBV DNA was 40.9 ± 11.2 years (P = 0.05).ConclusionsOBI was prevalent among 50% of HBcAb positive healthy blood donors. The frequency of positive HBcAb among healthy HBsAg negative blood donors was comparable to previous studies reported from Iran. On the other hand, the frequency of HBV DNA in HBsAg negative blood donors was higher than previous reports.

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“…[1][2][3] Another possibility is that anti-HBc findings represented false positive results or resulted from interactive antibody, and that such individuals had never been exposed to HBV. 4 The prevalence of these cases can range from 0.1% to 32% in various surveys, [5][6][7][8][9][10][11][12] depending upon the prevalence of HBV infection and patients investigated.…”

Section: Introductionmentioning

confidence: 99%

Responses to hepatitis B vaccine in isolated anti-HBc positive adults

Yao

Ren

Chen

et al. 2016

Human Vaccines & Immunotherapeutics

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Immune responses of isolated anti-HBc subjects are not well characterized in populations in China. This study aimed to evaluate immune responses to hepatitis B vaccination in isolated anti-HBc positive subjects. A cohort of 608 subjects were selected and separated into isolated anti-HBc (negative for HBsAg and anti-HBs, positive for anti-HBc) and control (negative for HBsAg, anti-HBs, and anti-HBc) groups, who were matched by age and sex. All subjects received 3 doses of hepatitis B vaccine (20mg) at months 0, 1, and 3, followed by testing for serological responses 1 month after the third vaccination. The positive seroprotection rate and geometric mean titer (GMT) for hepatitis B surface antibody (anti-HBs) of isolated anti-HBc subjects were significantly lower than those in the control group(86.2% vs.92.1%, P D 0.02; 47.26 vs.97.81 mIU/mL, P < 0.001). When stratified by age, positive seroprotection rate in the isolated antiHBc group were 92%, 88.5% and 79.4% in the 20-34, 35-49, and 50-60 y old subgroups, respectively (x2 D 5.919, P D 0.04). Additionally, the GMT level for anti-HBs in the isolated anti-HBc group for different age subgroups were 104.43, 47.87 and 31.79 mIU/mL respectively (x2 D 19.44, P < 0.001). The GMT level for anti-HBc before vaccination were negatively correlated with GMT for anti-HBs after 3 doses of hepatitis B vaccine (r D ¡0.165, P < 0.001). In conclusion, isolated anti-HBc positive subjects can achieve good immune responses after hepatitis B vaccination, and the positive seroprotection rate and GMT level for anti-HBs were lower than the control group. Better responses could be observed in young adults, and significant negative correlations were found between GMT of anti-HBc before vaccination and GMT of anti-HBs after vaccination.

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Frequency of hepatitis B virus DNA in anti-HBc positive, HBsAg negative blood donors in Rasht, northern Iran

Cited by 16 publications

References 15 publications

Hepatitis B Vaccine Reduced the Prevalence of Antibodies to Hepatitis B Core Antigen in Blood Donors in Aseer Region,Saudi Arabia

Hepatitis B Vaccine Reduced the Prevalence of Antibodies to Hepatitis B Core Antigen in Blood Donors in Aseer Region,Saudi Arabia

Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

Responses to hepatitis B vaccine in isolated anti-HBc positive adults

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