Frequency of<i>BCL2</i>and<i>BCL6</i>translocations in follicular lymphoma: Relation with histological and clinical features

Díaz-Alderete, Andrea; Doval, Angela Alvárez; Camacho, Francisca I.; Verde, L. Sanchez; Sabı́n, Pilar; Arranz-Sáez, Reyes; Bellas, Carmen; Corbacho, Carlos; Gil, Juana; Perez-Martín, María; Ruiz-Marcellán, María C.; Gonzalez, Leocricia; Montalbán, Carlos; Piris, Miguel Á.; Menárguez, Javier

doi:10.1080/10428190701742472

Cited by 42 publications

(30 citation statements)

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“…39 It is also suggested that BCL6 rearrangements could be related to adverse clinical outcomes. 33,40 In our series, we observed that rearrangement in 21 cases (20%; n ¼ 105). DiazAlderete et al, 40 studying follicular lymphoma cases for BCL2 and BCL6 rearrangements by FISH on paraffin-embedded tissue sections, found positivity in 64 (101 þ /158) and 14% (22 þ /156) of the cases, respectively.…”

Section: Discussionsupporting

confidence: 48%

“…33,40 In our series, we observed that rearrangement in 21 cases (20%; n ¼ 105). DiazAlderete et al, 40 studying follicular lymphoma cases for BCL2 and BCL6 rearrangements by FISH on paraffin-embedded tissue sections, found positivity in 64 (101 þ /158) and 14% (22 þ /156) of the cases, respectively. They also distributed their cases into four groups: BCL2 À /BCL6 À (32%), BCL2 þ / BCL6 À (56%), BCL2 À /BCL6 þ (5%), and BCL2 þ / BCL6 þ (9%).…”

Section: Discussionsupporting

confidence: 48%

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Follicular lymphoma in young adults: a clinicopathological and molecular study of 200 patients

et al. 2013

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Follicular lymphoma is clinically heterogenous, and therefore necessitates the identification of prognostic markers to stratify risk groups and optimize clinical management. It is relatively rare in patients younger than 40 years, and the clinicopathologic characteristics and biological behavior in this age group are poorly understood. In the current study, samples from a cohort of 200 patients between 19 and 40 years were evaluated retrospectively with respect to clinical, histologic, and genetic features. These were then correlated with clinical outcome. The median age at presentation was 35 years with a slight female prepoderance (56%). Most of the cases are presented with nodal disease (90%). Concomitant follicular lymphoma and diffuse large B-cell lymphoma were observed in 7 (4%) patients. Immunohistologic studies showed the expression of CD10 (91%), BCL6 (97%), BCL2 (95%), MUM1/IRF4 (12%), MDM2 (17%), and CD23 (25%). BCL2 rearrangement was present in 74%, and BCL6 in 20%. The estimated overall survival of patients was 13 years (mean). The presence of anemia, elevated lactose dehydrogenase, bone marrow involvement, and high-risk follicular lymphoma international prognostic index correlated with adverse overall survival. Our findings revealed that follicular lymphoma in young adults demonstrate similarities with that of older adults, including the frequency of presentation at various anatomic sites, grade, and adverse prognostic factors.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionsupporting

confidence: 48%

Follicular lymphoma in young adults: a clinicopathological and molecular study of 200 patients

et al. 2013

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“…It has been reported that 88.5% of bcl-2/IgH rearrangements occur at the MBR breakpoint (7). The results suggest a higher rate of BM infiltration than other studies (8)(9)(10).…”

Section: Discussioncontrasting

confidence: 55%

Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells

et al. 2011

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Abstract. The purpose of this study was to investigate the roles of bcl-2 chromosomal translocation and Bcl-2 protein expression in follicular lymphoma (FL) minimal bone marrow (BM) infiltration. We identified the same bcl-2/ IgH fusion gene in paraffin-embedded lymph node (LN) samples and BM samples using immunohistochemistry (IHC), immunocytochemistry (ICC), cytologic morphology and fluorescence in situ hybridization (FISH). The presence of the Bcl-2/IgH fusion gene in the BM samples and paraffin-embedded LN samples from 56 patients with follicular lymphomas was detected using FISH. The Bcl-2 protein levels in BM and paraffin-embedded tissues were quantified using ICC and IHC, respectively. Approximately 78.6% (44/56) of the paraffin-embedded LN tissue sections that underwent FISH analysis had a bcl-2/IgH translocation. The primary lesion was also positive for the bcl-2/IgH fusion gene, as were the BM minimal infiltrates. The bcl-2/ IgH rearrangement occurred in 88.6% (39/44) of the BM specimens. The bcl-2/IgH recombination rate in stage III/IV cancers was significantly different to that observed in stage I/ II cancers (p=0.041). In 59% (23/39) of the cases with t(14;18), Bcl-2 was found to be present as assessed by ICC. Positive Bcl-2 ICC staining and the t(14;18) translocation (as detected using FISH) were positively correlated (p=0.028). We then applied the FISH method to slides that had previously been morphologically evaluated using Wright-Giemsa staining; any slides with at least one abnormal cell were subjected to FISH analysis following staining. The assessment of bcl-2/ IgH translocation status may contribute to the better detection of minimal BM infiltration by FL cells. Utilizing FISH and cytologic morphology techniques allows for earlier and more accessible BM examination.

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“…These data have been previously published. 12 Treatment protocols varied considerably within the series, over time, and among institutions. The protocols included 'watch and wait' approach, different regimens of chemotherapy, radiotherapy, and bone marrow transplantation as first-line therapy.…”

Section: Patientsmentioning

confidence: 99%

Improved demonstration of immunohistochemical prognostic markers for survival in follicular lymphoma cells

et al. 2011

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Follicular lymphoma (FL) is one of the most common forms of the low-grade non-Hodgkin's lymphoma in adults, with a characteristic translocation, t(14;18)(q32;q21) that deregulates the expression of the BCL2 gene. The clinical course of FL patients is variable, whereby a subset of patients survive for long periods even without relapses, whereas the majority have frequent relapses with shorter survival. We have analyzed a series of 186 FLs, studying the correlation between clinical outcome and the tumor cell expression of a set of immunohistochemical markers, using an automated procedure for tissue microarrays to reduce the subjectivity of scoring. The results identified several markers associated with differences in overall survival (OS) in univariate analyses, such as Cyclin E, Mdm2, CD10, p21, IgD, Bcl-xL, CD30, and E2F6. Cases with a higher level of expression of Cyclin E, Mdm2, p21, IgD, Bcl-xL, CD30, and E2F6 were associated with a significantly shorter OS. On the other hand, strong CD10 expression was linked to a significantly better outcome. A Cox model was then constructed, integrating the Follicular Lymphoma International Prognostic Index (FLIPI) score and a restricted selection of three immunohistochemical markers: Cyclin E, Mdm2, and CD10 expression. A potentially useful finding is that the integrated FLIPI plus immunohistochemical model can be used to identify a subset of 26 patients (almost 20% of the total series), with a survival probability of 100% at 5 years. This not only confirms that a group of FL cases may have a very good clinical course, but also indicates that this group can be identified using this integrated clinical and immunohistochemical approach.

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Frequency ofBCL2andBCL6translocations in follicular lymphoma: Relation with histological and clinical features

Cited by 42 publications

References 26 publications

Follicular lymphoma in young adults: a clinicopathological and molecular study of 200 patients

Follicular lymphoma in young adults: a clinicopathological and molecular study of 200 patients

Bcl-2/IgH expression in minimal bone marrow infiltration by follicular lymphoma cells

Improved demonstration of immunohistochemical prognostic markers for survival in follicular lymphoma cells

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