Frequency of micronuclei in population of Bhopal exposed to methyl isocyanate in 1984

Ganguly, Bani Bandana; Mandal, Shouvik; Kadam, Nitin N.

doi:10.1007/s13237-019-00300-4

Cited by 6 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, to conclude, though the cytogenetic changes are reported similar to earlier studies, it cannot be solely attributed to the exposure to toxic gas as many confounding factors may also contribute genetic damage but such studies are signi cant for assessing the risk (Ganguly and Mandal, 2017;Ganguly et al, 2019). Further, because of the complex interactions between environment, disease susceptibility and genetic susceptibility, the exploration of epigenetic mechanisms to meet challenges of CKD through novel ideas of molecular mechanisms is warranted.…”

Section: Discussionmentioning

confidence: 54%

“…The persistence of genomic instability in terms of higher chromosomal aberrations and atypical lymphocytes was also noticed in toxic gas exposed population of Bhopal (Malla et al, 2011;Senthilkumar et al, 2013). A pilot follow up study after 30 years of the tragedy by Ganguly et al,(2019) reported stable or clonal rearrangements even after 30 yearsin the increased SCE and decreased replicative index seen immediately after toxic gas exposure.It also demonstrated a correlation between age, exposure status and cytogenetic alterations in toxic gas exposed individuals.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of cytogenetic alterations of toxic gas exposed population of Bhopal having chronic kidney disease

Samarth

Tiwari²,

Modi³

et al. 2021

Preprint

View full text Add to dashboard Cite

The industrial disaster of Bhopal in 1984 resulted into widespread morbidity and mortality in the vicinity of the industry and required long term surveillance for chronic health effects in those affected by the leakage of gas. Although few cytogenetic studies were undertaken to assess genetic damage in survivors of the disaster, no studies are available on cytogenetic damage of toxic gas exposed population having chronic kidney disease (CKD).Thus, the present study aimed to evaluate cytogenetic alterations in chronic kidney disease patients who wereexposed to leaked gas and to compare it with those who were not exposed to the leaked gas. The cytogenetic alterations were evaluated through chromosomal aberration analysis and micronuclei assay. The study included 608 study participants divided into four groups on the basis of history of exposure to the leaked gas and presence or absence of CKD. The results of the study showed no statistically significant difference in cytogenetic damagebetween gas exposed and non-exposed patients of CKD. However, significantly higher cytogenetic damage was observed among gas exposed participants having CKD as compared to gas exposed participants free from CKD.Thus, to conclude though the cytogenetic alterations were observed in exposed group it cannot be solely attributed to the gas exposure and the role of other confounders must also be studied.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Evaluation of cytogenetic alterations of toxic gas exposed population of Bhopal having chronic kidney disease

Samarth

Tiwari²,

Modi³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Thirdly sequential measurement of cytogenetic parameters along with environmental measurement of various toxic pollutants would have given a better causal-effect relationship of toxic gas exposure with cytogenetic changes. Thus, to conclude, though the cytogenetic changes are reported similar to earlier studies, it cannot be solely attributed to the exposure to toxic gas as many confounding factors may also contribute to genetic damage but such studies are signi cant for assessing the risk [49]. Further, because of the complex interactions between environment, disease susceptibility, and genetic susceptibility, the exploration of epigenetic mechanisms to meet the challenges of CKD through novel ideas of molecular mechanisms is warranted.…”

Section: Discussionmentioning

confidence: 55%

Evaluation of Cytogenetic Alterations of Toxic Gas Exposed Population of Bhopal Having Chronic Kidney Disease

Samarth

Tiwari

Modi³

et al. 2021

Preprint

View full text Add to dashboard Cite

The industrial disaster of Bhopal in 1984 resulted in widespread morbidity and mortality in the vicinity of the industry and required long-term surveillance for chronic health effects in those affected by the leakage of gas. Although few cytogenetic studies were undertaken to assess genetic damage in survivors of the disaster, no studies are available on cytogenetic damage of toxic gas-exposed populations having chronic kidney disease (CKD). Thus, the present study aimed to evaluate cytogenetic alterations in chronic kidney disease patients who were exposed to leaked gas and to compare it with those who were not exposed to the leaked gas. The cytogenetic alterations were evaluated through chromosomal aberration analysis and micronuclei assay. The study included 608 study participants divided into four groups based on the history of exposure to the leaked gas and the presence or absence of CKD. The results of the study showed no statistically significant difference in cytogenetic damage between gas-exposed and non-exposed patients of CKD. However, significantly higher cytogenetic damage was observed among gas-exposed participants having CKD as compared to gas-exposed participants free from CKD. Thus, to conclude though the cytogenetic alterations were observed in an exposed group it cannot be solely attributed to the gas exposure and the role of other confounders must also be studied.

show abstract

“…However, immediate genetic effects reported by individual and small studies as part of the multi-center genetic screening conducted by ICMR has not been reported. Therefore, a genetic screening was required to extract information on the present genetic status of the exposed subjects and their progeny (Ganguly and Mandal 2017; Ganguly et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Spectrum of stable and unstable rearrangements in lymphocytic chromosomes investigated in Bhopal population thirty year post MIC-disaster

Ganguly

Mandal

Kadam

2022

Preprint

Self Cite

View full text Add to dashboard Cite

Immediate assessment of genetic damage in methyl isocyanate (MIC) gas exposed population in small and heterogeneous samples using diversified study designs and solid-stained metaphases could not depict the actual genetic impact of MIC on accidentally exposed individuals. The outcome of the then large multi-center genetic screening program was not available to the public and scientific community. Also the routine of the regular epidemiological health-survey does not capture the genetic and long-term effect of MIC. Therefore, genetic screening was carried out 30-years post disaster during 2015–2017 with a view to screen the present status of chromosomal consequences in lymphocytic cells. Participants were recruited from moderate (34) and severely (78) exposed, and unexposed (35) cohorts with their informed consent. Analysis of ~ 100 mitotic cells and karyotyping of at least 10–15 and all abnormal metaphases detected structural and numerical alterations, including stable and replicable ones. Clonal abnormalities were detected with monosomal and complex karyotype, trisomy 8, del5q/20q, loss of Y, etc. Among all, X-chromosome was frequently involved in numerical alterations. Structural aberrations appeared higher in the then exposed populations, though abnormalities cannot be linked directly to MIC-exposure 30-year post disaster. Collectively, all rearrangements were markedly higher in the severely exposed population. Altogether, the detected abnormalities appeared random and indicated genomic instability, suggesting follow-up at shorter intervals for the individuals detected with clonal aberrations. G-banding has facilitated recognition of chromosomal involvement and their breakpoints, and classification of structural rearrangements. The present data has been derived from the 30-years post-disaster genetic screening.

show abstract

Frequency of micronuclei in population of Bhopal exposed to methyl isocyanate in 1984

Cited by 6 publications

References 26 publications

Evaluation of cytogenetic alterations of toxic gas exposed population of Bhopal having chronic kidney disease

Evaluation of cytogenetic alterations of toxic gas exposed population of Bhopal having chronic kidney disease

Evaluation of Cytogenetic Alterations of Toxic Gas Exposed Population of Bhopal Having Chronic Kidney Disease

Spectrum of stable and unstable rearrangements in lymphocytic chromosomes investigated in Bhopal population thirty year post MIC-disaster

Contact Info

Product

Resources

About