Frequency of sister chromatid exchange and hematological effects in pesticide-exposed greenhouse sprayers

Cytogenetic damage in female Pakistani agricultural workers exposed to pesticides

“…These differences may be due to the fact that these studies were conducted on different populations, with different types and levels of exposure, and that the use of protective measures varied between studies. Published data indicate that individuals not using proper safety measures are at high risk of genetic damage and increased MN frequency [Rupa et al, 1989;Kourakis et al, 1992;Lander and Ronne, 1995]. Only a slight increase in genotoxicity was observed where protective equipment was used [Pastor et al, 2002b].…”

Increased Sister Chromatid Exchange in Peripheral Blood Lymphocytes from Humans Exposed to Pesticide: Evidence Based on a Meta-analysis

“…We conducted the sensitivity analysis and found that the study including Bauchinger et al (1982), Carbonell et al (1993), Costa et al (2006), Gomez-Arroyo et al (1992), Hoyos et al (1996), Joksic et al (1997), Kourakis et al (1996), Lander et al (1995), Linnaimaa (1983), Pasquini et al (1996), Scarpato et al (1996), Steenland et al (1997) and Suarez et al (2003) was homogenous for SCE among total population, and the Q value for test of heterogeneity was 18.67 (df=12, p=0.097) (Figure 3). …”

“…Data were combined using either a fixed-effects model or a random-effects model Carbonell (Carbonell et al, 1990) 1990 Agricultural workers More than 10 years Spain Smoking Carbonell (Carbonell et al, 1993) 1993 Agricultural workers 170.7 ± 179.1 hours per year Spain Agricultural activity and duration Costa (Costa et al, 2006) 2006 Agricultural explorations and Range from 0.5 to 48 years Portugal Sex and smoking greenhouses c Ergene (Ergene et al, 2007) 2007 Subjects living in region 34.56±10.47 years Turkey Smoking contaminated with pesticides De Ferrari (De Ferrari et al, 1991) 1991 Floriculturists Unknown Italy Age and smoking Gomez-Arroyo 1992 Agricultural workers Range from 1 to 35 years Mexico Smoking, drinking (Gomez-Arroyo et al, 1992) and duration Gomez-Arroyo (Gomez-Arroyo et al, 2000) 2000 Floriculturists in greenhouses 7.7±3.8 years Mexico Sex c Hatjian (Hatjian et al, 2000) 2000 Agricultural college Short exposure Unite Kingdom students dipping sheep Hoyos (Hoyos et al, 1996) 1996 Farm workers 16.5±8.8 years Colombia Age, cigarette smoking and drinking Jablonicka (Jablonicka et al, 1989) 1989 Pesticides production Unknown Czechoslovakia Unknown Joksic (Joksic et al, 1997) 1997 Agricultural workers 12.1± 6.02 years Yugoslavia Unknown Kourakis (Kourakis et al, 1996) 1996 Pesticides sprayers Unknown Greece Unknown Lander (Lander et al, 1995) 1995 Greenhouse sprayers An average of 17 (range 1-50) years Denmark Smoking and age Linnaimaa (Linnainmaa, 1983) 1983 (Padmavathi et al, 2000) 2000 Pesticide industry…”

“…Sister chromatid exchange (SCE) in human peripheral blood lymphocytes is one of the most extensively studied biomarkers of cytogenetic damage. However, the results from epidemiological studies remained inconsistent and controversial (Bauchinger et al, 1982;Linnainmaa, 1983;Rupa et al, 1988;Jablonicka et al, 1989;De Ferrari et al, 1991;Rupa et al, 1991;Gomez-Arroyo et al, 1992;Carbonell et al, 1993;Lander et al, 1995;Hoyos et al, 1996;Kourakis et al, 1996;Pasquini et al, 1996;Scarpato et al, 1996;Joksic et al, 1997;Steenland et al, 1997;Gomez-Arroyo et al, 2000;Hatjian et al, 2000;Padmavathi et al, 2000;Shaham et al, 2001;Zeljezic et al, 2002;Suarez et al, 2003;Costa et al, 2006;Ergene et al, 2007;Rowland et al, 2007;Martinez-Valenzuela et al, 2009). In order to obtain a precise estimate of the association of SCE frequency with pesticide exposure, we collected published data to evaluate the validation of SCE in human peripheral blood lymphocytes as a cytogenetic biomarker of pesticideexposed population.…”

Pesticides: Genotoxic Risk of Occupational Exposure

“…In addition to SCE analysis, the BrdU differential staining technique can be used to assess the effects of pesticides in cell replication through the cell proliferation kinetics (CPK) (Gómez-Arroyo et al, 2000). In studies in which SCE has been used to detect exposure risk to pesticides, results have varied: they were positive with correlation between the frequency and the exposure time (Rupa et al, 1991b;Padmavathi et al, 2000;Shaham et al, 2001;Martínez-Valenzuela et al, 2009); they were positive without correlation (Rupa et al, 1988(Rupa et al, , 1991aLander & Rønne, 1995;Scarpato et al, 1996); they were not determined (Jabloniká et al, 1989;De Ferrari et al, 1991;Dulout et al, 1992;Ascarrunz et al, 2006), and they were negative (Steenland et al, 1986;Carbonell et al, 1990;1993;El-Ghazali et al, 1990;Gómez-Arroyo et al, 1992;Hoyos et al, 1996;Kourakis et al, 1996;Pasquini et al, 1996;Joksić et al, 1997).…”