Frequency signature of water activity by biospeckle laser

Cardoso, Rafael; Costa, Anderson Gomide; Nobre, C.M.B.; Braga, Roberto A.

doi:10.1016/j.optcom.2011.01.003

Cited by 50 publications

(31 citation statements)

References 22 publications

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“…Thus, the complexity of biological tissues or cells represents great challenge to identify or isolate the major phenomenon responsible for the boiling effect in speckle changes (Cardoso et al, 2011;Braga et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

When noise became information: State-of-the-art in biospeckle laser

Braga

Alves²

2017

Ciênc. agrotec.

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Laser was presented to science and industry in the 1960s and shortly became a useful tool in many areas, with applications based on its multiple characteristics such as coherence of light, which presents a phenomenon known as interference pattern, or speckle, when beam returns from an illuminated surface. Despite great application of speckle pattern, its residual presence, for example, in interferometric approaches was considered as a noise, demanding filtering. However, grains themselves became information as their dynamic changes in time started to be linked to biological sample activity. Dynamic laser speckle has been since then a phenomenon widely used to monitor biological activities in many areas from agriculture to medicine. It is known as biospeckle laser (BSL) when adopted in biological material, with high sensitivity to follow very tiny movements in biological tissues, linked to changes in speckle provided by scatterer activities inside and outside cells. Since the 1970s, biospeckle laser usage follows a crescent technologic spiral where technological developments opened room for new applications, while new demands regarding biological monitoring forced the development of new methodologies. Therefore, potential adoption of the phenomenon as a sensor, for instance, in agricultural and medical processes, as well as constant offer of new devices provided new turns in the BSL technologic spiral and opened room for technique improvement. In this study, I present a short history of biospeckle laser (BSL) with applications and development associated with challenges regarding its usage in portable and accessible devices or even in commercial equipment. And the history was packed in a temporal diagram identifying the breakpoints responsible for improvements in the use of the technique.Index terms: Optical metrology; biosystems; challenges. RESUMOO laser foi apresentado à ciência e à indústria em 1960, e rapidamente tornou-se uma ferramenta útil à muitas áreas do conhecimento, que fazem uso de suas múltiplas características, como é o caso da coerência da luz, que é responsável pelo fenômeno conhecido como padrão de interferência, ou speckle; este padrão de interferência ocorre quando a luz retorna do material iluminado formando uma figura de interferência para o observador. Apesar da grande aplicação, a presença residual do padrão de speckle em abordagens interferométricas apresentavase como um ruído que demanda filtragem. Apesar da dificuldade em alguns casos, os grãos de speckle tornaram-se fonte de informação em situações dinâmicas, quando suas mudanças começaram a ser relacionadas à atividade do material biológico iluminado. Speckle laser dinâmico tornou-se o termo usado para denominar o fenômeno que passou a ser usado para monitorar a atividade biológica e não biológica em aplicações desde a agricultura até aquelas relacionadas à medicina. Este fenômeno também é conhecido como biospeckle laser (BSL), quando aplicado em material biológico, e com grande sensibilidade para seguir os menores movimentos ...

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Section: Introductionmentioning

confidence: 99%

When noise became information: State-of-the-art in biospeckle laser

Braga

Alves²

2017

Ciênc. agrotec.

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“…When live objects are illuminated by laser the speckle pattern changes due to temporal changes of scattered wave phases and amplitudes. Speckle dynamics was used to assess the biological activity of seeds [2], fruits [3] and other objects. In [4] speckle contrast imaging was used to assess patients' blood flow in retinae and skin-supplying capillaries of their extremities.…”

Section: Introductionmentioning

confidence: 99%

Application of speckle image correlation for real-time assessment of metabolic activity in herpes virus-infected cells

Vladimirov

Malygin

Mikhailova

et al. 2014

J. Phys.: Conf. Ser.

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Abstract. Earlier we reported developing a speckle interferometry technique and a device designed to assess the metabolic activity of a cell monolayer cultivated on a glass substrate. This paper aimed at upgrading the technique and studying its potential for real-time assessment of herpes virus development process. . Speckle dynamics was recorded in the image plane of intact and virus-infected cell monolayer. HLE-3, L-41 and Vero cells were chosen as research targets. Herpes simplex virus-1-(HSV-1)-infected cell cultures were studied. For 24 h we recorded the digital value of optical signal I in one pixel and parameter  characterizing change in the distribution of the optical signal on 1010-pixel areas. The coefficient of multiple determination calculated by time dependences for three intact cell cultures equals 0.94. It was demonstrated that the activity parameters are significantly different for intact and virus-infected cells. The difference of value for intact and HSV-1-infected cells is detectable 10 minutes from the experiment start.

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“…The biospeckle has been used as a technique to measure detailed extensions of pine roots [3] or even the biological activity of roots in tissue culture [4], in assessing the water activity in maize and beans seeds [5], to studies of the relationship between chlorophyll pigments present in apples and their respective biological activity [6], and several other papers.…”

Section: Introductionmentioning

confidence: 99%

“…An alternative of the graphical and numerical classifications is a signal analysis in the time domain or in the frequency domain [5].…”

Section: Introductionmentioning

confidence: 99%

Comparison between Fourier and Wavelets Transforms in Biospeckle Signals

Mariano¹,

Braga²,

Sáfadi³

et al. 2013

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The dynamic speckle is a non-destructive optical technique that has been used as a tool for the characterization of the biological activity and several studies are conducted to obtain for more information about the correspondence of the observed phenomena and their expressions in the interference images. Analysis in the frequency domain has been considered as powerful alternative, and although there are works using Fourier transform in the frequency analysis of the biospeckle signals, the majority presents the wavelet transform as tool for spectral analysis. In turn, there are still doubts if the Fourier transform is not enough for the analysis of the biospeckle, which would enable the reduction of processing time since an operation is computationally simpler. In this context, the present study aims to compare the constituents' parts of the speckle signal according to Fourier and wavelet transforms for numerical analysis. The comparative analysis based on the absolute values of the differences technique (AVD) was carried out for performance evaluation of the Fourier and wavelet transforms, in which the speckle signals were decomposed spectrally and subsequently reconstructed with the elimination of specific frequency bands. Results showed that the wavelet transform allowed more information about signals constituents of the dynamic speckle, emphasizing its use instead of the Fourier transform, which in turn was restricted the situations in which the only interest is to know the spectral content of the data.

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Frequency signature of water activity by biospeckle laser

Cited by 50 publications

References 22 publications

When noise became information: State-of-the-art in biospeckle laser

When noise became information: State-of-the-art in biospeckle laser

Application of speckle image correlation for real-time assessment of metabolic activity in herpes virus-infected cells

Comparison between Fourier and Wavelets Transforms in Biospeckle Signals

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