Frequent DNA Polymorphisms Exist in Inbred CBA/J and C3H/HeN Mice

Yuan, Bo; Shum-Siu, Alice; Lentsch, Eric M.; Hu, Ling Hong; Hendler, F. J.

doi:10.1006/geno.1996.0592

Cited by 3 publications

(8 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymorphisms also are maintained in some inbred strains in spite of many generations of inbreeding (Yuan et al 1996). We looked for ratio variations within strains for the clones that we initially identified by their variation among strains.…”

Section: Genome Research 305mentioning

confidence: 99%

Mapping segmental and sequence variations among laboratory mice using BAC array CGH

Snijders¹,

Nowak²,

Huey³

et al. 2005

Genome Res.

View full text Add to dashboard Cite

We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation (|log2ratio| ≥ 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes (n = 16) or multiple sites on one chromosome (n = 1). We also show that small ratio changes (Δ log2ratio ∼ 0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.

show abstract

Section: Genome Research 305mentioning

confidence: 99%

Mapping segmental and sequence variations among laboratory mice using BAC array CGH

Snijders¹,

Nowak²,

Huey³

et al. 2005

Genome Res.

View full text Add to dashboard Cite

show abstract

“…Microsatellite markers at the Tyr, D7Mit98, Hbb, tyrosine hydroxylase (Th), and mammary tumor integration site 2 (Int2) A loci on Chr 7; the alcohol dehydrogenase class 1 (Adh1) locus on Chr 3; the minisatellite-6 hypermutable (Ms6hm) locus on Chr 4; the neuronal cell adhesion molecule (Ncam) locus on Chr 9; and the glutamine synthetase (Glns) locus on Chr 11 (Table 1) were informative in the liver DNA of individual CBA/J mice [23]. Microsatellite markers at the parathyroid hormone (Pth), O 6 -methyltransferase (Mmet), and Int2B loci were used as controls.…”

Section: Microsatellite Markersmentioning

confidence: 99%

“…The D7Mit98 primer sequences are from the Massachusetts Institute of Technology mouse genome database. All other primer sequences were designed in our laboratory [23]. In each pair, the upper sequence is the GenBank or reported sequence primer, and the lower sequence is a complementary primer sequence 3' to the upper sequence.…”

Section: Development Of Tumorsmentioning

confidence: 99%

“…Nineteen tumors were identified and segregated into those with no Hras1 mutations (n = 6), those heterozygous for Hras1 (n = 6), and those with LOH of Hras1 (n = 7) [3]. *Twelve previously identified microsatellite markers on five chromosomes were used to evaluate allelic loss during 4NQO-induced turmorigenesis [23]. † The primer sequences for Int2 and GIns were published previously [45].…”

Section: Development Of Tumorsmentioning

confidence: 99%

“…The identification of stable microsatellite polymorphisms distributed throughout the genome in CBA/ J mice provided informative markers for establishing boundaries of LOH [23]. Here, we report the use of these markers to identify allelic loss of the distal portion of Chr 7 in tumors with LOH at Hras1.…”

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Consistent allelic loss on mouse chromosome 7 distal to tyrosinase in 4-nitroquinoline-1-oxide-induced oral cavity tumors with loss of heterozygosity at Ha-ras-1

Yuan¹,

Hu²,

Lentsch³

et al. 1997

Mol. Carcinog.

Self Cite

View full text Add to dashboard Cite

We have previously shown that all CBA/J mice exposed to 4-nitroquinoline-1-oxide (4NQO) eventually develop oral cavity squamous cell carcinomas, and two-thirds of these tumors have Ha-ras-1 (Hras1) point mutations at codon 12. Half of the tumors with Hras1 mutations have loss of heterozygosity (LOH) at Hras1. In the study reported here, seven tumors with LOH at Hras1, six heterozygous for Hras1, and six without Hras1 mutations were analyzed to define the extent of LOH on chromosome (Chr) 7. Microsatellite polymorphisms present in CBA/J mice were used as informative allelic markers. Tumors with LOH at Hras1 showed consistent allelic loss at the distal portion of Chr 7. The boundary of allelic loss lay between the tyrosinase and hemoglobin beta chain loci, which are 6 cM apart. None of the tumors that remained heterozygous for Hras1 or had no Hras1 mutations had evidence of chromosomal loss involving Chr 7. Because LOH was only detected in advanced lesions long after exposure to 4NQO had ceased, we presume that the chromosomal alterations by which LOH occurred were independent of the carcinogen exposure. The development of LOH in only half of the tumors with Hras1 point mutations suggests that LOH was not caused by the initial Hras1 point mutation but was a highly selected event during tumorigenesis.

show abstract

PCR-detected genome polymorphism in malignant cell growth

Shvemberger¹,

Alexandrova²

2000

International Review of Cytology

View full text Add to dashboard Cite

Frequent DNA Polymorphisms Exist in Inbred CBA/J and C3H/HeN Mice

Cited by 3 publications

References 1 publication

Mapping segmental and sequence variations among laboratory mice using BAC array CGH

Mapping segmental and sequence variations among laboratory mice using BAC array CGH

Consistent allelic loss on mouse chromosome 7 distal to tyrosinase in 4-nitroquinoline-1-oxide-induced oral cavity tumors with loss of heterozygosity at Ha-ras-1

PCR-detected genome polymorphism in malignant cell growth

Contact Info

Product

Resources

About