Frequent HPV-independent p16/INK4A overexpression in head and neck cancer

Abstract

RPPA data suggest high p16 protein expression in many HPV (-) non-oropharyngeal HNSCCs, limiting its potential utility as an HPV biomarker outside of the oropharynx. HPV-independent overexpression of wild-type p16 in non-oropharyngeal HNSCC may be linked to global deregulation of chromatin state by inactivating mutations in NSD1.

Periodontal pathogens are a risk factor of oral cavity squamous cell carcinoma, independent of tobacco and alcohol and human papillomavirus

“…This was recently suggested in a report by Lechner et al 47 We also used 16S rRNA gene sequencing of the V3 and V4 regions of bacterial16S rRNA genes to identify the bacteria in the oral rinse specimens. Our sample size of the oral cavity and premalignant cohorts is small and therefore our results should be corroborated on a larger group of patients.…”

“…However, it is possible that in oral cancer, p16 immunohistochemistry may not be quite as reliable as in oropharyngeal cancer as a marker for HPV positivity. This was recently suggested in a report by Lechner et al 47 We also used 16S rRNA gene sequencing of the V3 and V4 regions of bacterial16S rRNA genes to identify the bacteria in the oral rinse specimens. New next generation sequencing techniques using metagenomics analysis of WGS sequences generated by lllumina HiSeq are now available which can give a more comprehensive identification of bacteria in oral rinse specimens.…”

Review of emerging biomarkers in head and neck squamous cell carcinoma in the era of immunotherapy and targeted therapy

“…Approximately one third of HNSCCs express p16. p16 is a widely used clinical biomarker for HPV—a well‐known cause of HNSCC—and HPV status, in turn, is an important prognostic marker used for patient stratification in HNSCC . Notably, in the most recent American Joint Committee on Cancer (AJCC) cancer staging book (2017), a distinct staging system for HNSCC patients with positive p16 expression was recommended.…”

“…p16 is a widely used clinical biomarker for HPV-a well-known cause of HNSCC-and HPV status, in turn, is an important prognostic marker used for patient stratification in HNSCC. [95][96][97] Notably, in the most recent American Joint Committee on Cancer (AJCC) cancer staging book (2017), 75 a distinct staging system for HNSCC patients with positive p16 expression was recommended. The p16 protein is generally evaluated by immunohistochemistry (IHC), and HPV infection by DNA/mRNA polymerase chain reaction (PCR).…”

Developing Classifications of Laryngeal Dysplasia: The Historical Basis

“…Most molecular/genetic studies have been performed on invasive laryngeal carcinoma, and often on head and neck squamous cell carcinomas in general, as well as in relation to the role of human papillomavirus (HPV) status (e.g., [73][74][75][76][77]). Only a few molecular/genetic studies have been performed on SILs.…”