FRESH bioprinting of biodegradable chitosan thermosensitive hydrogels

Rahimnejad, Maedeh; Adoungotchodo, Atma; Demarquette, Nicole R.; Lerouge, Sophie

doi:10.1016/j.bprint.2022.e00209

Cited by 14 publications

(18 citation statements)

References 51 publications

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“…The support bath must maintain a shear‐thinning behavior in order not to hinder the movement of the bioprinting needle. [ 22 ] The viscosity of the support bath was studied as a function of applied shear rate. All albumin foam groups showed a general shear‐thinning behavior, as demonstrated in Figure 2C,D.…”

Section: Resultsmentioning

confidence: 99%

“…during long gelation processes, e.g., thermal gelation. [22,23] Furthermore, some bath removal mechanisms can be detrimental to cell viability and structural fidelity, especially when lowering the temperature or mechanical agitation is the only way to remove the bath. [24] Moreover, removing gel support from cavities and confined parts of the printed structure can be cumbersome.…”

Section: Introductionmentioning

confidence: 99%

“…Primarily, the limited availability of dissolved oxygen and nutrient diffusion in the bioprinted cell‐laden constructs before the bath removal may cause necrosis or hypoxia‐induced apoptosis, which is a major obstacle to keeping the cells viable during long gelation processes, e.g., thermal gelation. [ 22,23 ] Furthermore, some bath removal mechanisms can be detrimental to cell viability and structural fidelity, especially when lowering the temperature or mechanical agitation is the only way to remove the bath. [ 24 ] Moreover, removing gel support from cavities and confined parts of the printed structure can be cumbersome.…”

Section: Introductionmentioning

confidence: 99%

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In‐Foam Bioprinting: An Embedded Bioprinting Technique with Self‐Removable Support Bath

Madadian,

Ravanbakhsh,

Touani Kameni

et al. 2024

Small Science

Self Cite

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The emergence of embedded three‐dimensional (3D) bioprinting has revolutionized the biofabrication of free‐form constructs out of low‐viscosity and slow‐crosslinking hydrogels. Using gel‐based support baths has limitations including lack of proper oxygenation and nutrition and complications with bath removal. Herein, a novel‐embedded 3D bioprinting technique is developed with an albumin foam support bath as a promising substitute. The proposed technique, in‐foam bioprinting, offers excellent printability and convenience in bath removal while providing cells with easy access to oxygen and nutrients. The foam‐based support bath is characterized through foam stability and rheological tests. The bubble size in the foam is measured to study the change in the structure of the bath due to the coalescence of the bubbles over time. Free‐form structures are successfully 3D printed with thermoresponsive chitosan‐based bioinks to demonstrate the capability of the in‐foam bioprinting technique. The viability of bioprinted fibroblast L929 cells is studied over a seven‐day period, showing high cell viability of over 97%, which is attributed to the abundance of oxygen and nutrition in the foam support bath. Importantly, in‐foam bioprinting is beneficial for biofabricating large samples with a long printing time without jeopardizing cell viability.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In‐Foam Bioprinting: An Embedded Bioprinting Technique with Self‐Removable Support Bath

Madadian,

Ravanbakhsh,

Touani Kameni

et al. 2024

Small Science

Self Cite

View full text Add to dashboard Cite

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“…For instance, Rahimnejad et al applied the FRESH approach with a warm sacrificial bath made of Pluronic to successfully enhance the bioprintability of thermosensitive chitosan hydrogels, which could become mechanically solid at body temperature but not instantaneous. 129 On the other hand, instead of directly printing hydrogel structures in a sacrificial bath, hydrogel structures printed in the gel bath could serve as sacrificial templates too. For example, sacrificial agarose fibers can be printed as vascular templates in GelMA, and then removed after the surrounding hydrogel were crosslinked to leave the hollow vascular structure inside for cell seeding purpose.…”

Section: Bioprintingmentioning

confidence: 99%

“…Besides, hydrogel‐based materials without quick crosslinking capability can also be printed with sacrificial baths. For instance, Rahimnejad et al applied the FRESH approach with a warm sacrificial bath made of Pluronic to successfully enhance the bioprintability of thermosensitive chitosan hydrogels, which could become mechanically solid at body temperature but not instantaneous 129 . On the other hand, instead of directly printing hydrogel structures in a sacrificial bath, hydrogel structures printed in the gel bath could serve as sacrificial templates too.…”

Section: Sacrificial Manufacturing Processesmentioning

confidence: 99%

Sacrificial biomaterials in 3D fabrication of scaffolds for tissue engineering applications

Wang,

Zhou

2023

J Biomed Mater Res

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Three‐dimensional (3D) printing technology has progressed exceedingly in the area of tissue engineering. Despite the tremendous potential of 3D printing, building scaffolds with complex 3D structure, especially with soft materials, still exist as a challenge due to the low mechanical strength of the materials. Recently, sacrificial materials have emerged as a possible solution to address this issue, as they could serve as temporary support or templates to fabricate scaffolds with intricate geometries, porous structures, and interconnected channels without deformation or collapse. Here, we outline the various types of scaffold biomaterials with sacrificial materials, their pros and cons, and mechanisms behind the sacrificial material removal, compare the manufacturing methods such as salt leaching, electrospinning, injection‐molding, bioprinting with advantages and disadvantages, and discuss how sacrificial materials could be applied in tissue‐specific applications to achieve desired structures. We finally conclude with future challenges and potential research directions.

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Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

Wu,

Yang,

Gupta

et al. 2024

Adv Funct Materials

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Embedded bioprinting overcomes the barriers associated with the conventional extrusion‐based bioprinting process as it enables the direct deposition of bioinks in 3D inside a support bath by providing in situ self‐support for deposited bioinks during bioprinting to prevent their collapse and deformation. Embedded bioprinting improves the shape quality of bioprinted constructs made up of soft materials and low‐viscosity bioinks, leading to a promising strategy for better anatomical mimicry of tissues or organs. Herein, the interplay mechanism among the printing process parameters toward improved shape quality is critically reviewed. The impact of material properties of the support bath and bioink, printing conditions, cross–linking mechanisms, and post‐printing treatment methods, on the printing fidelity, stability, and resolution of the structures is meticulously dissected and thoroughly discussed. Further, the potential scope and applications of this technology in the fields of bioprinting and regenerative medicine are presented. Finally, outstanding challenges and opportunities of embedded bioprinting as well as its promise for fabricating functional solid organs in the future are discussed.

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FRESH bioprinting of biodegradable chitosan thermosensitive hydrogels

Cited by 14 publications

References 51 publications

In‐Foam Bioprinting: An Embedded Bioprinting Technique with Self‐Removable Support Bath

In‐Foam Bioprinting: An Embedded Bioprinting Technique with Self‐Removable Support Bath

Sacrificial biomaterials in 3D fabrication of scaffolds for tissue engineering applications

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

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