Fresh layers of RNA-mediated regulation in Gram-positive bacteria

Bouloc, Philippe; Repoila, Francis

doi:10.1016/j.mib.2015.12.008

Cited by 43 publications

(31 citation statements)

References 59 publications

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“…On the one hand, heterologous expression of diverse Hfq proteins in Salmonella showed that members of this family have an intrinsic propensity to bind sRNAs 96 . On the other hand, the collective results of Hfq studies in Gram-positive bacteria so far argue against the prominent sRNA-mRNA matchmaking function that is a hallmark in Gram-negative bacteria 97 .…”

Section: Hfqmentioning

confidence: 99%

RNA-binding proteins in bacteria

2018

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RNA-binding proteins (RBPs) are central to most if not all cellular processes, dictating the fate of virtually all RNA molecules in the cell. Starting with pioneering work on ribosomal proteins, studies of bacterial RBPs have paved the way for molecular studies of RNA-protein interactions. Work over the years has identified major RBPs that act on cellular transcripts at the various stages of bacterial gene expression and that enable their integration into post-transcriptional networks that also comprise small non-coding RNAs. Bacterial RBP research has now entered a new era in which RNA sequencing-based methods permit mapping of RBP activity in a truly global manner in vivo. Moreover, the soaring interest in understudied members of host-associated microbiota and environmental communities is likely to unveil new RBPs and to greatly expand our knowledge of RNA-protein interactions in bacteria.

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Section: Hfqmentioning

confidence: 99%

RNA-binding proteins in bacteria

2018

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“…Cis-acting sRNAs form fully complementary interactions with their target mRNAs, whereas trans-acting sRNAs are only partly complementary to their partner mRNAs. In Gram-negative bacteria, the interaction between a trans-acting sRNA and its targets often relies on an RNA chaperone, such as Hfq, which promotes sRNA-mRNA duplex formation, whereas in Gram-positive bacteria, a role for Hfq in sRNA-mediated control is less clear [3].…”

Section: Hfq-binding Srnas -Recent Discoveriesmentioning

confidence: 99%

Small RNAs in major foodborne pathogens: from novel regulatory activities to future applications

Thorsing

Santos

Kallipolitis

2018

Current Opinion in Biotechnology

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Small regulatory RNAs (sRNAs) are involved in post-transcriptional control of important cellular processes and contribute to the success of a pathogen. Here, we use studies primarily selected from Salmonella enterica and Listeria monocytogenes to illustrate the current status of sRNA biology in important foodborne pathogens. We discuss how the regulatory activities of sRNAs can be affected by base pairing RNAs known as 'sponge RNAs', or by RNA-binding proteins, such as the newly discovered sRNA chaperone ProQ. Furthermore, we highlight recent findings for sRNAs with regulatory roles during infection, some of which are present in multiple copies, designated 'sibling sRNAs'. Importantly, knowledge on sRNA-mediated regulation can be exploited for biotechnological applications, such as in generating gene knockdowns to promote desired traits.

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“…Limiting accessibility of the RBS is therefore a common mechanism for gene regulation that bacteria have exploited in many different ways. There are several other recent reviews of focusing on diverse aspects of this area . RNA structure can be quite dynamic, and RNA structures have evolved to interpret changing conditions within the cell.…”

Section: Regulation Of Translation In Response To Stimulimentioning

confidence: 99%

The role of mRNA structure in bacterial translational regulation

Meyer

2016

WIREs RNA

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The characteristics of bacterial messenger RNAs (mRNAs) that influence translation efficiency provide many convenient handles for regulation of gene expression, especially when coupled with the processes of transcription termination and mRNA degradation. An mRNA's structure, especially near the site of initiation, has profound consequences for how readily it is translated. This property allows bacterial gene expression to be altered by changes to mRNA structure induced by temperature, or interactions with a wide variety of cellular components including small molecules, other RNAs (such as sRNAs and tRNAs), and RNA-binding proteins. This review discusses the links between mRNA structure and translation efficiency, and how mRNA structure is manipulated by conditions and signals within the cell to regulate gene expression. The range of RNA regulators discussed follows a continuum from very complex tertiary structures such as riboswitch aptamers and ribosomal protein-binding sites to thermosensors and mRNA:sRNA interactions that involve only base-pairing interactions. Furthermore, the high degrees of diversity observed for both mRNA structures and the mechanisms by which inhibition of translation occur have significant consequences for understanding the evolution of bacterial translational regulation. WIREs RNA 2017, 8:e1370. doi: 10.1002/wrna.1370 For further resources related to this article, please visit the WIREs website.

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Fresh layers of RNA-mediated regulation in Gram-positive bacteria

Cited by 43 publications

References 59 publications

RNA-binding proteins in bacteria

RNA-binding proteins in bacteria

Small RNAs in major foodborne pathogens: from novel regulatory activities to future applications

The role of mRNA structure in bacterial translational regulation

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