FRET‐Based Cellular Sensing with Genetically Encoded Fluorescent Indicators

Claussen, Jonathan C.; Hildebrandt, Niko; Medintz, Igor L.

doi:10.1002/9783527656028.ch10

Cited by 2 publications

(3 citation statements)

References 104 publications

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“…The nonradiative energy transfer depends on the overlap between the wave functions of the excited states of the donor and the acceptor, and therefore, it is directly related to the value of R DA . From the absorption, PL, PLQY measurements, and information about the measurement parameters, it is possible to calculate R 0 using the following equation: ,,, R 0 = 0.2108 true[ κ 2 normalΦ normalD 0 n − 4 ∫ 0 ∞ F normalD ( λ ) ε normalA ( λ ) λ 4 nobreak0em0.25em⁡ normald λ true] 1 / 6 where κ 2 is the orientation factor, Φ D 0 is the donor PLQY, n is the refraction index of the solvent, F D is the normalized spectral distribution of the donor emission, λ is the photon wavelength and ε A is the molar extinction coefficient of the acceptor. The constants used to calculate the Förster radius were: κ 2 = 2/3 , and n = 1.40496. , The PLQY was measured in solution using an integrating sphere, and the result was Φ D 0 = (61.9 ± 6.2)%; ε A (λ) was calculated from the absorption spectrum of L54, and the emission spectrum of L75 was used for F D (λ) and I D .…”

Section: Resultsmentioning

confidence: 99%

“…After calculating R 0 it is possible to calculate R DA by using the equation: ,,, R DA 6 = R 0 6 − true( 1 − I DA I normalD true) R 0 6 1 − I DA I D where I D is the emission of the donor and I DA is the emission of the donor in the presence of the acceptor. I D was obtained from the emission spectrum of L75, and I DA was obtained from the emission spectrum of the respective terpolymer or blend.…”

Section: Resultsmentioning

confidence: 99%

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Intramolecular Energy Transfer in the Terpolymer LaPPS76

dos S. Moraes,

Uhdre,

de J. Santana

et al. 2024

J. Phys. Chem. C

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Due to the advancements in the OLED technology, research on new materials as active layers for the production of OLED devices has grown steadily. The synthesis of new polymers has been investigated to obtain characteristics that optimize the intensity, durability, and emission of the spectra of the OLEDs in specific spectral regions. The terpolymer LaPPS76 (L76) has a structure that combines segments of copolymers fluorenebenzothiadiazole (L54) and fluorene-terpyridine (L75). This study characterized the emission of terpolymer L76 and quantified the energy transfer (ET) processes between these chromophores. Solutions of L76 diluted in tetrahydrofuran (THF) were prepared with two relative percentages of chromophores. Additionally, mixtures of copolymers L75 and L54 were prepared, in the same proportions as they appear in the terpolymer, to compare the emission properties of the blends with those of the terpolymers. The optical characterization of L76 revealed that internal ET occurs within the molecule, from fluorene-terpyridine (FT) to fluorene-benzothiadiazole (FB), and ET saturation is observed for higher percentages of FT. The comparison between the emission from the blend and the one from the terpolymer, as a function of the solution concentration, highlights the difference in transfer processes. The efficiency of ET was quantified through calculations based on the lifetime and Forster radius, both showing higher ET efficiency in the terpolymers. The material with the highest donor concentration achieved an ET efficiency of nearly 100%, as calculated using the Forster radius.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Intramolecular Energy Transfer in the Terpolymer LaPPS76

dos S. Moraes,

Uhdre,

de J. Santana

et al. 2024

J. Phys. Chem. C

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“…Excited electronic states play an important role in many chemical and physical processes. For multiple chromophore molecules found in the liquid, solid state, and sometimes gas phase, electronic excitations are affected by the interaction between excited states, known as the excitonic coupling. − It can be described in Förster resonance energy-transfer (FRET) models …”

Section: Introductionmentioning

confidence: 99%

Analysis of Site Energies and Excitonic Couplings: The Role of Symmetry and Polarization

Fedorov

2024

J. Phys. Chem. A

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An excitonic coupling model is developed based on an equation-of-motion coupled cluster combined with the fragment molecular orbital method. The effects of polarization and excitonic coupling on the splitting of quasi-degenerate levels in systems containing multiple chromophores are elucidated on dimers of formaldehyde, water, formic acid, hydrogen fluoride, and carbon monoxide. It is shown that the level structure is mainly determined by the mutual polarization of chromophores and to a lesser extent by the excitonic coupling. The role of symmetry in excitonic coupling in dimers is discussed. The excitonic coupling between all residues in the photoactive yellow protein (PDB: 2PHY) is analyzed.

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FRET‐Based Cellular Sensing with Genetically Encoded Fluorescent Indicators

Cited by 2 publications

References 104 publications

Intramolecular Energy Transfer in the Terpolymer LaPPS76

Intramolecular Energy Transfer in the Terpolymer LaPPS76

Analysis of Site Energies and Excitonic Couplings: The Role of Symmetry and Polarization

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