FRET-based ratiometric detection of Hg2+ and biothiols using naphthalimide–rhodamine dyads

Abstract

A new naphthalimide-rhodamine-based dyad in CH(3)CN-HEPES (1 : 1) buffer solution exhibits fluorescence resonance energy transfer (FRET) from naphthalimide to the rhodamine moiety on addition of only Hg(2+) ions and allows ratiometric absorption and fluorimetric estimation of Hg(2+) ions between 50 nM (10 ppb) to 2 μM (0.4 ppm). FRET-induced fluorescence changes were recovered again by the subsequent addition of thiol amino acids via reverse FRET. The interconversion of probe and via the complexation/decomplexation by the modulation of Hg(2+)/Cys exhibited a selective probe for biothiols in real samples.

Resonance energy transfer-based fluorescent probes for Hg2+, Cu2+and Fe2+/Fe3+ions

“…10). 29 Further, FRET-induced uorescence changes in 10 were recovered again by the subsequent addition of thiol amino acids. The similar FRET pair as chemosensor 11 was reported by Guo et al for detecting Hg 2+ and works over a broad pH range of 5.7-11.0 (Fig.…”

Synthesis of Fluorogenic Chemosensors for Hg2+Detection Using Naphthalimide Derivatives

“…FRET signal mechanism also can be employed for the design of naphthalimide-rhodamine-based chemosensor, in which the fluorescence detection errors due to backscattering effects from the excitation source can be efficiently avoided (30). Therefore, strong red emission (approximately at 585 nm) of rhodamine due to FRET from excitation of the 1,8-naphthalimide could be observed (42)(43)(44).…”

A new FRET ratiometric fluorescent chemosensor for Hg2+ and its application in living EC 109 cells

“…Because the pseudoStokes shifts of FRET based probes are larger than the Stokes shifts of either the donor or acceptor dyes, the possible self-quenching as well as fluorescence detection errors due to backscattering effects of the excitation source will be efficiently avoided [26]. However, despite many advantages, examples of the ratiometric fluorescence probes for Hg 2+ based on FRET are considerably limited [27][28][29][30][31][32].…”