FRET probe for detecting two mutations in one EGFR mRNA

Abstract

Technologies for visualizing and tracking RNA are essential in molecular biology, including in disease-related fields. In this study, we propose a novel probe set (DAt-probe and T-probe) that simultaneously detects...

An endoplasmic reticulum-targeted fluorescent probe for ratiometric tracking of endogenous SO2 derivatives

“…16–19 Recently, the design mechanisms for functional fluorescent probes have been mainly based on intramolecular charge transfer (ICT), 20–23 through-bond energy transfer (TBET), 24,25 Förster resonance energy transfer (FRET), 26,27 photo-induced electron transfer (PET) 28,29 and excited-state intramolecular proton transfer (ESIPT). 30,31 Although FRET-based ratiometric probes with various superiorities such as large Stokes shift, and facile design and synthesis were developed, 32–36 ER-targeted FRET-based probes for the monitoring of HSO 3 − /SO 3 2− are rarely developed (Table S1, ESI†).…”