Friction-Controlled Traction Force in Cell Adhesion

Pompe, Tilo; Kaufmann, Martín; Kasimir, Maria; Johne, Stephanie; Glorius, Stefan; Renner, Lars D.; Bobeth, M.; Pompe, W.; Werner, Carsten

doi:10.1016/j.bpj.2011.08.027

Cited by 42 publications

(43 citation statements)

References 34 publications

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“…13,14 Our results agree with recent experimental and theoretical work 31,32 . 14 Within the first 2 h of cell adhesion of human umbilical cord vein endothelial cells (HUVEC), different regimes of spreading and traction force generation could be revealed.…”

Section: Introductionsupporting

confidence: 91%

Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Müller

Pompe

2016

Soft Matter

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Cell adhesion is regulated by the mechanical characteristics of the cell environment. The influences of different parameters of the adhesive substrates are convoluted in the cell response leading to questions on the underlying mechanisms, like biochemical signaling on the level of adhesion molecules, or viscoelastic properties of substrates and cell. By a time-resolved analysis of traction force generation during early cell adhesion, we wanted to elucidate the contributions of substrate mechanics to the adhesion process, in particular the impact of substrate elasticity and the molecular friction of adhesion ligands on the substrate surface. Both parameters were independently adjusted by (i) an elastic polyacrylamide hydrogel of variable crosslinking degree and (ii) a thin polymer coating of the hydrogel surface controlling the affinity (and the correlated substrate-ligand friction) of the adhesion ligand fibronectin. Our analysis showed two sequential regimes of considerable force generation, whose occurrence was found to be independent of substrate properties. The first regime is characterized by spreading of the cell and a succeeding force increase. After spreading cells enter the second regime with saturated forces. Substrate elasticity and viscosity, namely hydrogel elasticity and ligand affinity, were both found to affect the kinetics and absolute levels of traction force quantities. A faster increase and a higher saturation level of traction forces were observed for a higher substrate stiffness and a higher ligand affinity. The results complement recent modeling approaches on the evolution of forces in cell spreading and contribute to a better understanding of the dynamics of cell adhesion on viscoelastic substrates.

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Section: Introductionsupporting

confidence: 91%

Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Müller

Pompe

2016

Soft Matter

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“…al. propose a model of friction-controlled traction forces, in which focal adhesions are motile, and the friction of adhesion movement generates traction forces [154]. …”

Section: Mechanobiology Of Adhesion Revealed Using Supported Lipidmentioning

confidence: 99%

Supported lipid bilayer platforms to probe cell mechanobiology

Glazier¹,

Salaita²

2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Mammalian and bacterial cells sense and exert mechanical forces through the process of mechanotransduction, which interconverts biochemical and physical signals. This is especially important in contact-dependent signaling, where ligand-receptor binding occurs at cell-cell or cell-ECM junctions. By virtue of occurring within these specialized junctions, receptors engaged in contact-dependent signaling undergo oligomerization and coupling with the cytoskeleton as part of their signaling mechanisms. While our ability to measure and map biochemical signaling within cell junctions has advanced over the past decades, physical cues remain difficult to map in space and time. Recently, supported lipid bilayer (SLB) technologies have emerged as a flexible platform to measure and manipulate membrane receptor mechanotransduction, allowing one to mimic cell-cell junctions. Changing the lipid composition and underlying substrate tunes bilayer fluidity, and lipid and ligand micro- and nano-patterning spatially control positioning and clustering of receptors. Patterning metal gridlines within SLBs introduces corrals that confine lipid mobility and introduce mechanical resistance. Here we review fundamental SLB mechanics and how SLBs can be engineered as tunable cell substrates for mechanotransduction studies. Finally, we highlight the impact of this work in understanding the biophysical mechanisms of cell adhesion.

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“…In contrast to elastic substrates where deformations come to a halt when cell tractions reach a steady state, cell adhesion ligands anchored to viscoelastic or plastic substrates remain mobile and thus provide a different mechanical stimulus. It has been shown that cellular traction forces decrease with increasing mobility of adhesion ligands anchored non-covalently to different polymeric substrates [21], although the bulk mechanical properties of the polymeric substrates were not characterized in that report.…”

Section: Introductionmentioning

confidence: 99%

Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

et al. 2014

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Cell behavior such as cell adhesion, spreading, and contraction critically depends on the elastic properties of the extracellular matrix. It is not known, however, how cells respond to viscoelastic or plastic material properties that more closely resemble the mechanical environment that cells encounter in the body. In this report, we employ viscoelastic and plastic biomembrane-mimicking cell substrates. The compliance of the substrates can be tuned by increasing the number of polymer-tethered bilayers. This leaves the density and conformation of adhesive ligands on the top bilayer unaltered. We then observe the response of fibroblasts to these property changes. For comparison, we also study the cells on soft polyacrylamide and hard glass surfaces. Cell morphology, motility, cell stiffness, contractile forces and adhesive contact size all decrease on more compliant matrices but are less sensitive to changes in matrix dissipative properties. These data suggest that cells are able to feel and respond predominantly to the effective matrix compliance, which arises as a combination of substrate and adhesive ligand mechanical properties.

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Friction-Controlled Traction Force in Cell Adhesion

Cited by 42 publications

References 34 publications

Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Supported lipid bilayer platforms to probe cell mechanobiology

Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

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