Friends in low places: Soil derived microbial inoculants for biostimulation and biocontrol in crop production

Johnston‐Monje, David; Arévalo, Ashly; Rojas, Ana Cristina Bolanõs

doi:10.1016/b978-0-12-822122-8.00020-0

Cited by 7 publications

(6 citation statements)

References 169 publications

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“…Furthermore, microbial inoculation significantly increased the grape yield (kg/vine) by 2- to 3-fold as compared to the untreated control under pathogenic stress of E. necator across the locations. These results are in agreement with the other researchers who reported that microbial inoculants have a positive impact on the yield of grapes grown under the pathogenic stress of E. necator (El-Mogy, 2017 ; Johnston-Monje et al, 2021 ; Laurent et al, 2021 ). Dario et al ( 2008 ) stated that commercial formulations of Bacillus subtilis , namely, Serenade and Milastin K, showed effective and consistent suppression of E. necator under greenhouse and field conditions.…”

Section: Discussionsupporting

confidence: 93%

A Comparative Analysis of Microbe-Based Technologies Developed at ICAR-NBAIM Against Erysiphe necator Causing Powdery Mildew Disease in Grapes (Vitis vinifera L.)

et al. 2022

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Globally, Erysiphe necator causing powdery mildew disease in grapevines (Vitis vinifera L.) is the second most important endemic disease, causing huge economic losses every year. At present, the management of powdery mildew in grapes is largely dependent upon the use of chemical fungicides. Grapes are being considered as one of the high pesticide-demanding crops. Looking at the residual impact of toxic chemical pesticides on the environment, animal, and human health, microbe-based strategies for control of powdery mildew is an emerging technique. It offers an environment-friendly, residue-free, and effective yet safer approach to control powdery mildew disease in grapes. The mode of action is relatively diverse as well as specific to different pathosystems. Hence, the aim of this study was to evaluate the microbe-based technologies, i.e., Eco-pesticide®, Bio-Pulse®, and Bio-Care 24® developed at the Plant-Microbe Interaction and Rhizosphere Biology Lab, ICAR-NBAIM, Kushmaur, against grape powdery mildew and to integrate these technologies with a safer fungicide (sulfur) to achieve better disease control under organic systems of viticulture. The experiments were conducted at four different locations, namely, the vineyards of ICAR-NRCG, Rajya Draksha Bagayatdar Sangh (MRDBS), and two farmers' fields at Narayangaon and Junnar in the Pune district of Maharashtra. A significantly lower percent disease index (PDI) was recorded on the leaves of grape plants treated with Eco-Pesticide®/sulfur (22.37) followed by Bio-Pulse®/sulfur (22.62) and Bio-Care 24®/sulfur (24.62) at NRCG. A similar trend was observed with the lowest PDI on bunches of Eco-pesticide®/sulfur-treated plants (24.71) followed by Bio-Pulse®/sulfur (24.94) and Bio-Care®/sulfur (26.77). The application of microbial inoculants singly or in combination with sulfur has a significant positive impact on the qualitative parameters such as pH, total soluble solids (TSS), acidity, berry diameter, and berry length of the grapes at different locations. Among all the treatments, the Bio-Pulse®/sulfur treatment showed the highest yield per vine (15.02 kg), which was on par with the treatment Eco-Pesticide®/sulfur (14.94). When compared with the yield obtained from the untreated control, 2.5 to 3 times more yield was recorded in the plants treated with either of the biopesticides used in combination with sulfur. Even in the case of individual inoculation, the yield per vine was approximately two times higher than the untreated control and water-treated plants across the test locations. Results suggested that microbial technologies not only protect grapevines from powdery mildew but also enhance the quality parameters with increased yield across the test locations.

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Section: Discussionsupporting

confidence: 93%

A Comparative Analysis of Microbe-Based Technologies Developed at ICAR-NBAIM Against Erysiphe necator Causing Powdery Mildew Disease in Grapes (Vitis vinifera L.)

et al. 2022

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“…Because tissue culturing attempts to create sterile plantlets, while vegetatively propagated plants are denied sexual reproduction, the resulting propagules may be nearly devoid of their normal vertically transmitted microbiome and likewise nearly devoid of the accompanying phenotypic benefits that seed endophytes would provide. Because many endophytes are thought to be horizontally transmitted (Johnston-Monje et al, 2021a), environmental variation can also influence microbial diversity in the endosphere. This has been shown in grapes, where the majority of bacterial endophytes in roots were observed to originate and vary in relation to the soil microbiome, which in turn was influenced by soil moisture, carbon, temperature, and geographic location (Zarraonaindia et al, 2015).…”

Section: Endosphere Microbiome Contribution To Terroirmentioning

confidence: 99%

Plant microbiomes as contributors to agricultural terroir

Johnston-Monje,

Vergara,

Lopez-Mejia

et al. 2023

Front. Agron.

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Agricultural products such as tea, chocolate, coffee and wine are valued for their sensorial and nutritional qualities. Variation in the growing conditions of a crop can influence the plant’s phenotype, thus it behooves agriculturalists to optimize the conditions on their farms to grow the highest quality product. The set of growing conditions associated with a certain geographic location and its influence on the product’s chemistry is known as terroir. Although terroir plays a significant role in marketing and consumer appreciation as well as product identity and valorization, rarely are the biochemical differences or the factors creating them very well understood. The word derives from the Latin for “land”, suggesting terroir is simply a function of the geographical location where a plant grew, while in its modern usage, terroir is understood to be the result of soil type, climate, landscape, topography, biotic interactions and agricultural practice. Except for fermented food products like wine and chocolate, plant associated microbiomes have been little studied for their contribution to a crop’s terroir; however, modern metagenomics and metabolomics technologies have given scientists the tools to better observe how microbial diversity can impact the chemical variation in plant products. Differences in the microbiomes inhabiting plant organs can change phytochemistry by altering host metabolism, for example increasing the nutrients absorbed by roots that then are deposited in leaves, seeds and fruits. Plant associated microbes can consume plant molecules, removing them from the metabolome, or they can contribute smells and flavors of their own. This review aims to synthesize research into rhizosphere, endosphere, phyllosphere, spermosphere, carposphere, and anthosphere microbiome influences on plant biochemistry and crop derived products, while helping to increase the appreciation that beneficial microbes are able to contribute to agriculture by improving phytochemical quality.

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“…Modern plants are considered holobionts; an amalgam of different microbes that have coevolved with the host to better survive and cope with numerous biotic and abiotic stresses [1]. Amongst the numerous beneficial plant-associated microbes, the most famous are arbuscular mycorrhizal fungi, which extend through the soil to increase the absorptive Life 2022, 12, 1372. https://doi.org/10.3390/life12091372 https://www.mdpi.com/journal/life area of the root (in 90% of plant species); and nitrogen fixing bacteria colonizing the roots of leguminous plants [2]. Other classical examples of beneficial plant-microbe interactions include the stress resistance conferred to grasses by seed-transmitted Epichloë fungi [3] and biocontrol of take-all disease in wheat rhizospheres by antibiotic-producing strains of Pseudomonas bacteria [4].…”

Section: Introductionmentioning

confidence: 99%

Stochastic Inoculum, Biotic Filtering and Species-Specific Seed Transmission Shape the Rare Microbiome of Plants

Johnston‐Monje

Gutiérrez

López-Lavalle

2022

Life

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A plant’s health and productivity is influenced by its associated microbes. Although the common/core microbiome is often thought to be the most influential, significant numbers of rare or uncommon microbes (e.g., specialized endosymbionts) may also play an important role in the health and productivity of certain plants in certain environments. To help identify rare/specialized bacteria and fungi in the most important angiosperm plants, we contrasted microbiomes of the seeds, spermospheres, shoots, roots and rhizospheres of Arabidopsis, Brachypodium, maize, wheat, sugarcane, rice, tomato, coffee, common bean, cassava, soybean, switchgrass, sunflower, Brachiaria, barley, sorghum and pea. Plants were grown inside sealed jars on sterile sand or farm soil. Seeds and spermospheres contained some uncommon bacteria and many fungi, suggesting at least some of the rare microbiome is vertically transmitted. About 95% and 86% of fungal and bacterial diversity inside plants was uncommon; however, judging by read abundance, uncommon fungal cells are about half of the mycobiome, while uncommon bacterial cells make up less than 11% of the microbiome. Uncommon-seed-transmitted microbiomes consisted mostly of Proteobacteria, Firmicutes, Bacteriodetes, Ascomycetes and Basidiomycetes, which most heavily colonized shoots, to a lesser extent roots, and least of all, rhizospheres. Soil served as a more diverse source of rare microbes than seeds, replacing or excluding the majority of the uncommon-seed-transmitted microbiome. With the rarest microbes, their colonization pattern could either be the result of stringent biotic filtering by most plants, or uneven/stochastic inoculum distribution in seeds or soil. Several strong plant–microbe associations were observed, such as seed transmission to shoots, roots and/or rhizospheres of Sarocladium zeae (maize), Penicillium (pea and Phaseolus), and Curvularia (sugarcane), while robust bacterial colonization from cassava field soil occurred with the cyanobacteria Leptolyngbya into Arabidopsis and Panicum roots, and Streptomyces into cassava roots. Some abundant microbes such as Sakaguchia in rice shoots or Vermispora in Arabidopsis roots appeared in no other samples, suggesting that they were infrequent, stochastically deposited propagules from either soil or seed (impossible to know based on the available data). Future experiments with culturing and cross-inoculation of these microbes between plants may help us better understand host preferences and their role in plant productivity, perhaps leading to their use in crop microbiome engineering and enhancement of agricultural production.

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Friends in low places: Soil derived microbial inoculants for biostimulation and biocontrol in crop production

Cited by 7 publications

References 169 publications

A Comparative Analysis of Microbe-Based Technologies Developed at ICAR-NBAIM Against Erysiphe necator Causing Powdery Mildew Disease in Grapes (Vitis vinifera L.)

A Comparative Analysis of Microbe-Based Technologies Developed at ICAR-NBAIM Against Erysiphe necator Causing Powdery Mildew Disease in Grapes (Vitis vinifera L.)

Plant microbiomes as contributors to agricultural terroir

Stochastic Inoculum, Biotic Filtering and Species-Specific Seed Transmission Shape the Rare Microbiome of Plants

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