Frog Embryo Teratogenesis Assay: Xenopus (FETAX) — A Short-Term Assay Applicable to Complex Environmental Mixtures

Dumont, James N.; Schultz, T.W.; Buchanan, Michelle V.; Kao, Glenn L.

doi:10.1007/978-1-4613-3611-2_27

Cited by 160 publications

(89 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite these noteworthy changes in the cellular components of the blood in various species exposed to TCDD, information on hematological toxicity of TCDD including histopathologic changes is relatively sparse. Xenopus embryos have been widely used to evaluate the teratogenic and toxic effects of chemicals (6,17 (18). At day 12 after fertilization (7 days after the end of exposure), 3 (14) and observed in a Hitachi H-800 electron microscope.…”

Section: Introductionmentioning

confidence: 99%

Apoptotic Cell Death of Erythrocytes in Xenopus Larvae Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin

et al. 1997

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Apoptotic Cell Death of Erythrocytes in Xenopus Larvae Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin

et al. 1997

View full text Add to dashboard Cite

show abstract

“…The standardized 96h assay Frog Embryo Teratogenesis Assay -Xenopus (FETAX) is often used as a screening test to determine embryotoxicity and the teratogenic risk of chemical agents (Dumont et al 1983). The aim of this study was to provide first experimental data on sublethal toxic effects of SCCPs (CP-12 mixture, 56% of chlorine) to frog embryos.…”

Section: (Phoca Hispida) Beluga Whales (Delphinapterus Leucas) and mentioning

confidence: 99%

Sublethal Toxic Effects and Induction of gGutathione S-transferase by Short-Chain Chlorinated Paraffins (SCCPs) and C-12 alkane (dodecane) in Xenopus laevis Frog Embryos

et al. 2006

View full text Add to dashboard Cite

Short chain chlorinated paraffins (SCCPs) are important industrial chemicals with high persistence in the environment but poorly characterized ecotoxicological effects. We studied embryotoxic effects of commercial mixture of SCCP (carbon length C-12, 56% of chlorine; CP56-12) and non-chlorinated n-alkane (dodecane, C-12) in the 96h Frog Embryo Teratogenesis Assay -Xenopus (FETAX). Only weak lethal effects were observed for both substances (the highest tested concentration 500 mg/L of both chemicals caused up to 11% mortality). On the other hand, we observed developmental malformations and reduced embryo growth at 5 mg/l and higher concentrations. However, the effects were not related to chlorination pattern as both SCCPs and dodecane induced qualitatively similar effects. SCCPs also significantly induced phase II detoxification enzyme glutathione S-transferase (GST) in Xenopus laevis embryos even at 0.5 mg/L, and this biomarker might be used as another early warning of chronic toxic effects. Our results newly indicate significant developmental toxicity of both SCCPs and n-dodecane to aquatic organisms along with inductions of specific biochemical toxicity mechanisms.

show abstract

“…Thus, X. laevis also serves as an excellent model to evaluate early embryonic development (Dumont et al, 1983;Fort et al, 1988;Fort et al, 1989;Fort and Bantle, 1990). Collection of simultaneous information on the effects on gametogenesis and reproductive outcome not only provides hazard assessment information, but also provides mechanistic clues concerning the modes of action of reproductive toxicants.…”

Section: Significance Of the Current Researchmentioning

confidence: 99%

Development of a Reproductive Toxicity Test Using Xenopus

Bantle¹

1998

View full text Add to dashboard Cite

Frog Embryo Teratogenesis Assay: Xenopus (FETAX) — A Short-Term Assay Applicable to Complex Environmental Mixtures

Cited by 160 publications

References 8 publications

Apoptotic Cell Death of Erythrocytes in Xenopus Larvae Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin

Apoptotic Cell Death of Erythrocytes in Xenopus Larvae Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin

Sublethal Toxic Effects and Induction of gGutathione S-transferase by Short-Chain Chlorinated Paraffins (SCCPs) and C-12 alkane (dodecane) in Xenopus laevis Frog Embryos

Development of a Reproductive Toxicity Test Using Xenopus

Contact Info

Product

Resources

About