From 3D Back to 2D Monolayer Stomach Organoids-on-a-Chip

Jantaree, Phatcharida; Bakhchova, Liubov; Steinmann, Ulrike; Naumann, Michael

doi:10.1016/j.tibtech.2020.11.013

Cited by 17 publications

(17 citation statements)

References 13 publications

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“…It is also possible to convert organoids into a monolayer by dissociating and seeding the cells on transwells in order to obtain 2D ALIs . The advantage of such a technique is the rapid generation of multiple homogeneous monolayers, consisting of the same diverse cell types previously present in the 3D cell culture, but with easier access to apical and basal surfaces, and regular exposure to medium . Airway cells maintained under these conditions self-organize in a more natural manner, replicating more consistently the in vivo respiratory epithelium, with the development of ciliated pseudostratified cylindrical epithelium and goblet cells. , A simplified visual description of the main steps to generate an organoid considering the starting point, cultivation methods, and 3D induction is in Figure .…”

Section: Generating Lung Organoidsmentioning

confidence: 99%

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Oliveira

Síbio

Costa

et al. 2021

ACS Biomater. Sci. Eng.

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The coronavirus disease 2019 (COVID-19), caused by the novel coronavirus, SARS-CoV-2, affects tissues from different body systems but mostly the respiratory system, and the damage evoked in the lungs may occasionally result in severe respiratory complications and eventually lead to death. Studies of human respiratory infections have been limited by the scarcity of functional models that mimic in vivo physiology and pathophysiology. In the last decades, organoid models have emerged as potential research tools due to the possibility of reproducing in vivo tissue in culture. Despite being studied for over one year, there is still no effective treatment against COVID-19, and investigations using pulmonary tissue and possible therapeutics are still very limited. Thus, human lung organoids can provide robust support to simulate SARS-CoV-2 infection and replication and aid in a better understanding of their effects in human tissue. The present review describes methodological aspects of different protocols to develop airway and alveoli organoids, which have a promising perspective to further investigate COVID-19.

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Section: Generating Lung Organoidsmentioning

confidence: 99%

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Oliveira

Síbio

Costa

et al. 2021

ACS Biomater. Sci. Eng.

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“…By using two glass layers with electrodes on the top and bottom, with a microfluidic channel cut through an additional sandwich layer in between, it is possible to fabricate sensor electrodes placed on two opposite sides ( Figure 14 ). Both configurations are suitable for protein detection and other biomedical applications [ 389 ].…”

Section: Impedance Spectroscopy Microfluidic Techniques and Methods F...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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Proteins in biological fluids (blood, urine, cerebrospinal fluid) are important biomarkers of various pathological conditions. Protein biomarkers detection and quantification have been proven to be an indispensable diagnostic tool in clinical practice. There is a growing tendency towards using portable diagnostic biosensor devices for point-of-care (POC) analysis based on microfluidic technology as an alternative to conventional laboratory protein assays. In contrast to universally accepted analytical methods involving protein labeling, label-free approaches often allow the development of biosensors with minimal requirements for sample preparation by omitting expensive labelling reagents. The aim of the present work is to review the variety of physical label-free techniques of protein detection and characterization which are suitable for application in micro-fluidic structures and analyze the technological and material aspects of label-free biosensors that implement these methods. The most widely used optical and impedance spectroscopy techniques: absorption, fluorescence, surface plasmon resonance, Raman scattering, and interferometry, as well as new trends in photonics are reviewed. The challenges of materials selection, surfaces tailoring in microfluidic structures, and enhancement of the sensitivity and miniaturization of biosensor systems are discussed. The review provides an overview for current advances and future trends in microfluidics integrated technologies for label-free protein biomarkers detection and discusses existing challenges and a way towards novel solutions.

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“…To learn more about the involvement of NHE1 in gastrointestinal growth and differentiation, absorption and secretion, in barrier function and microbiome regulation would be of importance, given the fact that NHE1 inhibition is discussed as a strategy for curbing tumor growth and/or its invasive properties in a large variety of organs and tumor cell types ( Stock et al, 2012 ; Harguindey et al, 2013 ; Meehan et al, 2017 ; Guan et al, 2018 ; Hyun et al, 2019 ; Iorio et al, 2020 ; Tamtaji et al, 2020 ; Greco et al, 2021 ; Mo et al, 2021 ), as well as for organ protection during ischemia ( Jung et al, 2006 ; Lee et al, 2009 ; Doods and Wu, 2013 ; Karmazyn, 2013 ; Wu et al, 2013 ; Sasamori et al, 2014 ), and as an anti-inflammatory and barrier-protective strategy ( Khan et al, 2005 ; Farkas et al, 2010 ; Wu and Qi, 2012 ; Yang et al, 2013 ; Monet et al, 2016 ; Zhang et al, 2018 ; Dubaniewicz et al, 2021 ). However, the recent advancement in the generation and maintenance of epithelium-derived organoids from all gastrointestinal organs with preservation of their organ- and site specific function ( Co et al, 2021 ; Gómez and Boudreau, 2021 ; Jantaree et al, 2021 ; Puschhof et al, 2021 ; Shiota et al, 2021 ), now opens an avenue to learn more about the role of NHE1 in the development and differentiation pattern of gastrointestinal epithelial cells, about its involvement in transepithelial transport of nutrients and electrolytes, and about its importance in microbial resistance and barrier function. First studies are published that demonstrate a differential expression of acid/base transporters along with absorptive or secretory ion channels or exchangers during the differentiation of human ( Yin et al, 2018 ; Zomer-van Ommen et al, 2018 ; Zhou et al, 2021 ) or mouse ( Nikolovska et al, 2022 ) intestinal organoids.…”

Section: Nhe1 In the Gastrointestinal Tractmentioning

confidence: 99%

The Role of Plasma Membrane Sodium/Hydrogen Exchangers in Gastrointestinal Functions: Proliferation and Differentiation, Fluid/Electrolyte Transport and Barrier Integrity

2022

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The five plasma membrane Na+/H+ exchanger (NHE) isoforms in the gastrointestinal tract are characterized by distinct cellular localization, tissue distribution, inhibitor sensitivities, and physiological regulation. NHE1 (Slc9a1) is ubiquitously expressed along the gastrointestinal tract in the basolateral membrane of enterocytes, but so far, an exclusive role for NHE1 in enterocyte physiology has remained elusive. NHE2 (Slc9a2) and NHE8 (Slc9a8) are apically expressed isoforms with ubiquitous distribution along the colonic crypt axis. They are involved in pHi regulation of intestinal epithelial cells. Combined use of a knockout mouse model, intestinal organoid technology, and specific inhibitors revealed previously unrecognized actions of NHE2 and NHE8 in enterocyte proliferation and differentiation. NHE3 (Slc9a3), expressed in the apical membrane of differentiated intestinal epithelial cells, functions as the predominant nutrient-independent Na+ absorptive mechanism in the gut. The new selective NHE3 inhibitor (Tenapanor) allowed discovery of novel pathophysiological and drug-targetable NHE3 functions in cystic-fibrosis associated intestinal obstructions. NHE4, expressed in the basolateral membrane of parietal cells, is essential for parietal cell integrity and acid secretory function, through its role in cell volume regulation. This review focuses on the expression, regulation and activity of the five plasma membrane Na+/H+ exchangers in the gastrointestinal tract, emphasizing their role in maintaining intestinal homeostasis, or their impact on disease pathogenesis. We point to major open questions in identifying NHE interacting partners in central cellular pathways and processes and the necessity of determining their physiological role in a system where their endogenous expression/activity is maintained, such as organoids derived from different parts of the gastrointestinal tract.

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From 3D Back to 2D Monolayer Stomach Organoids-on-a-Chip

Cited by 17 publications

References 13 publications

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Airway and Alveoli Organoids as Valuable Research Tools in COVID-19

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

The Role of Plasma Membrane Sodium/Hydrogen Exchangers in Gastrointestinal Functions: Proliferation and Differentiation, Fluid/Electrolyte Transport and Barrier Integrity

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