From a BODIPY–rhodamine scaffold to a ratiometric fluorescent probe for nitric oxide

Water-soluble Hantzsch ester as switch-on fluorescent probe for efficiently detecting nitric oxide

“…In addition, a linear relationship between the fluorescent intensity and the concentrations of NO was deduced with a correction coefficient of 0.998. Meanwhile, the calculated lowest limit detection (LOD, 3 σ/slope) was determined to be about 18 nM, which is similar with the values in literatures [37,42,45,46], suggesting that probe DHPS is a highly sensitive agent for detecting NO.…”

“…After addition of NO into the solution of DHPS, the fluorescence emission increased rapidly, and it became plateaued in 30 min. Although the response time is a bit longer in comparison with some reported NO probes (the response time for Cou-Rho-NO [45] and BRP-NO [46] was about 30 and 5 min, respectively), it is still an eminent reactive probe. For the further application in the biological samples, the influence of pH on the fluorescence of probe DHPS and compound PYS had to be taken into account.…”

Using photochemistry to understand and control the production of reactive oxygen species in biological environments

“…[70] Likewise, other probes based on the same concept were engineered for trapping NO  and H 2 O 2 . [62,69] …”

“…Such probes could be achieved by linking a donor molecule to either a fluorescein in its lactone form or a spirolactam rodhamine. [62,69,70] Upon interaction with ROS, ring opening of the latter molecules will occur, enabling them to act as acceptor. Using this strategy, a probe for the detection of HOCl was achieved by attaching a coumarin moiety (donor) to a rhodamine-thiosemicarbazide molecule.…”

“…7). [62][63][64][65][66][67][68][69][70] In the overall turn on, the probes are made of a donor molecule linked to a suitable acceptor molecule, which does not exhibit fluorescence (Fig. 7a).…”