Every year, over 30,000 international students study in regional Australia, 1 in urban centres that lack the intercultural resources and cross-cultural literacies of metropolitan cities. The University of Southern Queensland (USQ) supports and brings together over 26,000 students studying both on campus and online, including a diverse international student population of 7,000 students. The university's enrolment of international on-campus students is the second highest in the Regional Universities Network, of which USQ is a member. 2 This article analyses the experiences of international students as they encounter an unfamiliar Australian culture in the context of studying in Toowoomba, the regional city where USQ has its main campus. These students' experiences of engaging, becoming familiar with and mastering new and unfamiliar cultural practices and academic literacies provides insight into the processes of acculturation that students undergo as they make their transition to life in regional Australia, both at university and in Queensland communities.The research reported here draws on evidence collected as part of a larger research study conducted in a first-year undergraduate nursing course at USQ. The course is one of two designed to assist students to develop the literacies and practices they need to succeed as learners in their higher education studies and as nursing professionals. The study was conducted from semester 1, 2006 to semester 2, 2013. Its aim was to investigate the experiences of students over the first semester of their study at university.This article documents the qualitative findings collected over two semesters of the larger study, which focused on international students' experiences as they made their transition to the regional community. The data were obtained through forum discussion posts as well as assignments that included students' reflections about their learning strengths and weaknesses; the strategies they used to enhance their study; their communication, cross-communication and online skills development; and their end-of-semester reflections.The thematic analysis of the qualitative data generated findings that outline the discomfort experienced by students as they confronted the regional culture. Not only did they find the new regional culture different, but they were unprepared for the diversity they discovered there. However, the data also reveal that students' use of reflective, communicative and critical practice assisted them to engage more confidently with the new culture and community.