From dipeptides to oligopeptides: Analysis by capillary electrophoresis/electrospray mass spectrometry

Wycherley, Darren; Rose, Malcolm E.; Giles, Kevin; Jarvis, S.; McDowall, Mark

doi:10.1002/bmc.1130090618

Biomedical Chromatography

1995

DOI: 10.1002/bmc.1130090618

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From dipeptides to oligopeptides: Analysis by capillary electrophoresis/electrospray mass spectrometry

Darren Wycherley

Malcolm E. Rose

Kevin Giles³

et al.

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1998

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Cited by 3 publications

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“…demonstrated that MS can be the most powerful detector not only for HPLC but for CE (generally capillary zone electrophoresis, CZE) as well; many technical improvements , and applications (e.g. refs and ) have been published (for a review, see ref ). But from our own experiences, it became clear that some technical details have to be carefully optimized to make this interface routine or a part of an automated analytical tool.…”

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confidence: 99%

Characterization of a Capillary Zone Electrophoresis/Electrospray-Mass Spectrometry Interface

Siethoff¹,

Nigge²,

Linscheid³

1998

Anal. Chem.

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A dependable and stable CZE/ESI-MS interface has been constructed. To avoid instabilities in both, the capillary electrophoretic separation and the electrospray, the second of the three concentric capillaries in the three-layered sprayer has been replaced by an aluminum-coated fused-silica capillary with an inner diameter only slightly greater than the outer diameter of the separation capillary. By this means, the otherwise often observed destruction of the separation capillary ("electrodrilling") can be avoided completely due to the suppression of electrochemical processes leading to gas bubble formation at the tip of the sprayer. With some examples taken from different biochemical areas and by separation of natural compounds, the capability and the reliability of the modified sprayer as the central part of the interface are demonstrated.

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mentioning

confidence: 99%

Characterization of a Capillary Zone Electrophoresis/Electrospray-Mass Spectrometry Interface

Siethoff¹,

Nigge²,

Linscheid³

1998

Anal. Chem.

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High sensitivity analysis of proteins and peptides by capillary electrophoresis‐tandem mass spectrometry: Recent developments in technology and applications

1998

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Analytical biochemistry, in particular the analysis of regulatory proteins that control biological systems and pathways, is dependent on methods of ever-increasing sensitivity. Capillary electrophoresis (CE) has long been recognized as an ultrasensitive analytical technique. In spite of the high sensitivity, CE has not penetrated protein discovery research as a standard analytical method. In this review article we summarize recent technical developments which have significantly enhanced CE as a tool for the analysis of trace amounts of proteins. Specifically, we review recent advances in the development and application of capillary electrophoresis-mass spectrometry (CE-MS) and on-line analyte concentration techniques, and introduce the emerging field of microfluidics as a front end to mass spectrometry (MS).

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Liquid Chromatography-Mass Spectrometry: Bioanalytical Applications in PHarmacokinetics and Toxicokinetics.

Cheng¹,

Chasseaud²

1998

Drug Metabolism and Pharmacokinetics

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Summary: With the increasing need for reliable and comprehensive pharmacokinetic data from diverse non clinical as well as clinical studies, a sensitive and specific bioanalytical technique is essential . Such a tech nique is liquid chromatography-mass spectrometry (LC-MS). This article provides a brief overview of the key developments in LC-MS and focuses on the more recent LC-MS ionisation/interfacing technique , that of atmospheric pressure ionisation (API). The advantages of LC-API-MS/MS for routine bioanalysis , in terms of sensitivity, specificity and reproducibility are discussed. Recent scientific literature is cited that exemplifies the capabilities, applications and usefulness of this powerful analytical technique in pharmacoki netics/toxicokinetics studies.

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From dipeptides to oligopeptides: Analysis by capillary electrophoresis/electrospray mass spectrometry

Cited by 3 publications

References 0 publications

Characterization of a Capillary Zone Electrophoresis/Electrospray-Mass Spectrometry Interface

Characterization of a Capillary Zone Electrophoresis/Electrospray-Mass Spectrometry Interface

High sensitivity analysis of proteins and peptides by capillary electrophoresis‐tandem mass spectrometry: Recent developments in technology and applications

Liquid Chromatography-Mass Spectrometry: Bioanalytical Applications in PHarmacokinetics and Toxicokinetics.

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