From DNA barcoding to nanoparticle-based colorimetric testing: a new frontier in cephalopod authentication

Maggioni, Davide; Tatulli, Giuseppina; Montalbetti, Enrico; Tommasi, Nicola; Galli, Paolo; Labra, Massimo; Pompa, Pier Paolo; Galimberti, Andrea

doi:10.1007/s13204-020-01249-6

Cited by 9 publications

(7 citation statements)

References 54 publications

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“…Nevertheless, their requirements for long procedures and complex instrumentation make them unsuitable for rapid and portable testing. In an attempt to simplify the overall procedures, nanotechnologies have offered new perspectives in developing DNA barcoding-based hybrid techniques with naked-eye colorimetric readout, taking advantage of the plasmonic properties of gold nanoparticles [13,14]. A possible advancement toward the development of faster and portable technologies for on-site testing is represented by isothermal amplification techniques.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Colorimetric Assay for On-Site Authentication of Cephalopod Species

et al. 2020

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A colorimetric assay, exploiting the combination of loop-mediated isothermal amplification (LAMP) with DNA barcoding, was developed to address the authentication of some cephalopod species, a relevant group in the context of seafood traceability, due to the intensive processing from the fishing sites to the shelf. The discriminating strategy relies on accurate design of species-specific LAMP primers within the conventional 5’ end of the mitochondrial COI DNA barcode region and allows for the identification of Loligo vulgaris among two closely related and less valuable species. The assay, coupled to rapid genomic DNA extraction, is suitable for large-scale screenings and on-site applications due to its easy procedures, with fast (30 min) and visual readout.

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Section: Introductionmentioning

confidence: 99%

A Rapid Colorimetric Assay for On-Site Authentication of Cephalopod Species

et al. 2020

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“…Oleg N. Katugin and Anna O. Zolotova (e.g. Carlini and Graves, 1999;Takumiya et al, 2005;Dai et al, 2012;Wen et al, 2017;Maggioni et al, 2020;Afiati et al, 2022). It was shown earlier that partial sequencing of the CO1 gene marker (658 bp) can be successfully used for species identification in the family Gonatidae (Katugin et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…DNA barcoding based on the CO1 sequencing has proved to be an effective tool in distinguishing between different species in most of the main animal groups, including the phylum Mollusca (Folmer et al ., 1994; Anderson, 2000; Giribet et al ., 2006; Chen et al ., 2011), in particular, the class Cephalopoda (e.g. Carlini and Graves, 1999; Takumiya et al ., 2005; Dai et al ., 2012; Wen et al ., 2017; Maggioni et al ., 2020; Afiati et al ., 2022). It was shown earlier that partial sequencing of the CO1 gene marker (658 bp) can be successfully used for species identification in the family Gonatidae (Katugin et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

Species identification and genetic relationships in the squid family Gonatidae (Teuthida, Cephalopoda) based on partial sequencing of mitochondrial and nuclear genes

Katugin,

Zolotova

2023

J. Mar. Biol. Ass.

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Squids of the family Gonatidae are key components in oceanic communities. However, issues related to correct species identification, number of species, and their genetic relatedness remain. To address these issues, sequences from three mitochondrial (cytochrome c oxidase subunit I [CO1], 16S rRNA, and 12S rRNA) and two nuclear (18S and 28S) genes were analysed in the Gonatidae. Four of the five sequences (12S rRNA, 16S rRNA, 28S, and CO1) yielded rather similar patterns of genetic relationships among the species. Molecular evidence suggested intra-familial subdivision into two major groups of species having either five or seven longitudinal rows of teeth in the radula. The former group included all species of the genus Gonatus and two sister-species of Gonatopsis s. str. suggesting that all gonatid species with five rows of radular teeth represent a single taxonomic unit of a genus or subfamily level. Species with seven rows of radular teeth formed several ‘species’ clusters. Sequence analysis also addressed species identification issues in the Gonatidae. Two genetically divergent groups were found among squid which conformed to the description of Gonatus berryi. Molecular evidence suggested sister-species relationships between ‘large’ and ‘small’ forms of Boreoteuthis borealis with size-at-maturity as the only reported difference between these two cohorts. Sequence variation was observed within Gonatus pyros. Inclusion of gonatid sequences from the GenBank into the analysis suggested probable species misidentification in several cases. Combined use of several mitochondrial and nuclear sequences served as a valuable tool for species identification and provided a solid background for unravelling molecular genetic and taxonomic relationships in the Gonatidae.

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“…Similarly, 16 S rDNA-based methodologies elucidated clear success in identifying different cephalopod species included in seafoods. For example, it could be used to detect the flying squid Dosidicus gigas from cephalopod mixtures ( Ye et al, 2017 ) authenticate the common cuttlefish ( Sepia officinalis ) in seafood products in the Italian markets ( Maggioni et al, 2020 ), and many others. Hence, sequencing of the 16 S rDNA of frozen shellfishes was expected to provide success in accurate species identification.…”

Section: Discussionmentioning

confidence: 99%